Western Jilin Province is one of the world's three major saline–alkali land distribution areas, and is also an important area of global climate change and carbon cycle research. Rhizosphere soil microorganisms and enzymes are the most active components in soil, which are closely related to soil carbon cycle and can reflect soil organic carbon (SOC) dynamics sensitively. Soil inorganic carbon (SIC) is the main existing form of soil carbon pool in arid saline–alkali land, and its quantity distribution affects the pattern of soil carbon accumulation and storage. Previous studies mostly focus on SOC, and pay little attention to SIC. Illumina Miseq high-throughput sequencing technology was used to reveal the changes of community structure in three maize fields (M1, M2 and M3) and three rice fields (R1, R2 and R3), which were affected by different levels of salinization during soil development. It is a new research topic of soil carbon cycle in saline–alkali soil region to investigate the effects of soil microorganisms and soil enzymes on the transformation of SOC and SIC in the rhizosphere. The results showed that the root—soil—microorganism interaction was changed by saline–alkali stress. The activities of catalase, invertase, amylase and β-glucosidase decreased with increasing salinity. At the phylum level, most bacterial abundance decreases with increasing salinity. However, the relative abundance of Proteobacteria and Firmicutes in maize field and Firmicutes, Proteobacteria and Nitrospirae in rice field increased sharply under saline–alkali stress. The results of redundancy analysis showed that the differences of rhizosphere soil between the three maize and three rice fields were mainly affected by ESP, pH and soil salt content. In saline–alkali soil region, β-glucosidase activity and amylase were significantly positively correlated with SOC content in maize fields, while catalase and β-glucosidase were significantly positively correlated with SOC content in rice fields. Actinobacteria, Bacteroidetes and Verrucomicrobia had significant positive effects on SOC content of maize and rice fields. Proteobacteria, Gemmatimonadetes and Nitrospirae were positively correlated with SIC content. These enzymes and microorganisms are beneficial to soil carbon sequestration in saline–alkali soils.

Western Jilin province has the most serious area of soda salinization in Northeast China, which affects and restricts the sustainable development of agriculture. The effects of physico-chemical properties of rhizosphere and non-rhizosphere soil on soil microbial diversity and enzyme activities (polyphenol oxidase, catalase, invertase, amylase) were evaluated in typical soda saline-alkali paddy field. Community-level physiological profile (CLPP) based on Biolog-ECO plates was used to assess the functional diversity of soil microorganisms. Exchangeable sodium percentage (ESP) and pH were negative correlated with the microbial activity (AWCD), soil enzyme activities (amylase, sucrose, and catalase, except for polyphenol oxidase) in rice rhizosphere and non-rhizosphere soil (P < 0.05). The indexes of microbial diversity in rice rhizosphere soil were significantly higher than that of non-rhizosphere soil. The utilization of amino acids by rice rhizosphere microorganisms was relatively high, while non-rhizosphere soil had relatively high utilization of carboxylic acid, phenolic acid, and amine. Among the selected physico-chemical properties, soil organic carbon (SOC) and soil water content (SWC) had the greatest influence on the variation of microbial diversity indexes and enzyme activities in rhizosphere soil. ESP and pH showed a significant positive correlation with carbon source utilization, especially for amine (AM) and phenolic acid (PA) carbon source utilization (P < 0.05) by means of RDA, and the utilization rate of AM and PA carbon sources by rice rhizosphere and non-root soil microorganisms was P1 < P2 < P3.

Irrigation regime and fertilizer nitrogen (N) are considered as the most effective agricultural management systems to mitigate greenhouse gas (GHG) emissions from crop fields, but few studies have involved saline–alkaline paddy soil. Gas emitted from saline–alkaline paddy fields (1-year-old and 57-year-old) was collected during rice growing seasons by the closed chamber method. Compared to continuous flooding irrigation, lower average CH₄ flux (by 22.81% and 23.62%), but higher CO₂ flux (by 24.84% and 32.39%) was observed from intermittent irrigation fields. No significant differences of N₂O flux were detected. Application rates of N fertilizer were as follows: (1) No N (N0); (2) 60 kg ha⁻¹ (N60); (3) 150 kg ha⁻¹ (N150); and (4) 250 kg ha⁻¹ (N250). The cumulative emissions of GHG and N fertilizer additions have positive correlation, and the largest emission was detected at the rate of 250 kg N ha⁻¹ (N250). Global warming potential (GWP, CH₄ + N₂O + CO₂) of the 57-year-old field under the N250 treatment was up to 4549 ± 296 g CO₂-eq m⁻², approximately 1.5-fold that of N0 (no N application). In summary, the results suggest that intermittent irrigation would be a better regime to weaken the combined GWP of CH₄ and N₂O, but N fertilizer contributed positively to the GWP.

Soil organic carbon (SOC) plays a critical role in carbon cycling and soil quality of agroecosystems. Understanding the factors influencing SOC and the main indicators for soil quality can help in better soil management and sustainable agriculture. In this study, we selected three upland fields (U1, U2 and U3) and three paddy fields (P1, P2 and P3) of saline-alkali agroecosystems to study the impacts of soil physico-chemical properties (soil pH, exchangeable sodium percentage, electrical conductivity and bulk density) and enzyme activities (soil amylase, invertase, catalase and polyphenol oxidase) on SOC dynamics. The soil pH and exchangeable sodium percentage (ESP) had profoundly negative effect on SOC. Soil amylase and invertase activities were significantly positively correlated with SOC in both upland and paddy fields. Catalase promoted the accumulation of paddy SOC and polyphenol oxidase led to the acceleration of decomposition of upland SOC. Additionally, we combined SOC contents, soil physico-chemical properties and soil enzyme activities together to obtain the main indicators of soil quality. The results suggested that, in upland sites, the main factors affecting the soil quality were soil pH, ESP and SOC. As for paddy sites, the main indicators of soil quality were soil pH, amylase and invertase. By comparing the soil quality indicators between upland and paddy fields, it was observed that the inhibiting effect of ESP on paddy soil quality was not as significant as on upland soil quality due to the irrigation practice of rice planting, which could reduce the degree of soil alkalization. Therefore, paddy development has been widely used to improve the saline-alkali land in western Jilin Province of China.

Soil organic carbon (SOC) mineralization (conversion of carbonaceous material to carbon dioxide) plays a central role in global carbon cycle. However, the effects of SOC mineralization under different saline–alkali stress conditions are poorly understood. In order to understand the carbon mineralization processes, four paddy fields with different saline and alkali degrees were chosen as the experimental samples and the soil CO₂ emission fluxes at nine different time steps of the whole simulation experiment were observed. The physical and chemical properties of soils of four field conditions were compared for the dynamic changes of CO₂ flux in the progress of paddy field cultivation simulations. The results showed that the first three fields (P1, P2, and P3) were weakly alkaline soils and the last one (P4) was strongly alkaline soil. The SOC content of each plot was significantly different and there was a near-surface enrichment, which was significantly negatively correlated with the degree of alkalization. The accumulation process of the SOC mineralization during the incubation time was consistent with the first-order kinetic model. In the initial stage of mineralization, the amount of CO₂ released massively, and then the release intensity decreased rapidly. The mineralization rate decreased slowly with time and finally reached a minimum at the end of the incubation period. This study indicates that the SOC mineralization process is affected by a variety of factors. The main factors influencing SOC mineralization in the saline–alkaline soils are the exchangeable sodium percentage (ESP), followed by enzyme activities. Salinization of the soils inhibits the rate of soil carbon cycle, which has a greater impact on the carbon sequestration than on the carbon source process. The intensity and completeness of the SOC mineralization reactions increase with increasing SOC contents and decrease with increasing ESP levels.