Wheat is a vital crop globally, essential for agriculture, economics, and food security. However, in arid and semi-arid conditions, wheat production faces significant challenges due to low water availability, uneven rainfall distribution, and high soil salinity. The germination and early seedling stages are particularly vulnerable to these stresses. Therefore, this study assessed 15 wheat genotypes for their tolerance to salinity stress during early growth stages, using a hydroponic system with four salt stress levels (0, 50, 100, and 150 mM NaCl). Significant differences were observed for genotype and salinity main effects and their interaction on all investigated traits, indicating considerable variability in the response to salt stress among the investigated wheat cultivars. High NaCl concentrations led to substantial reductions in measured parameters across genotypes, with some showing resilience while others exhibited heightened sensitivity. Stress tolerance indices, such as mean productivity (MP), geometric mean productivity (GMP), harmonic mean (HM), stress tolerance index (STI) and yield index (YI), were identified as reliable indicators for selecting salt-tolerant wheat cultivars. Consequently, Sidi Okba (G11), Ziad (G12), Tamezghida (G13) and Zidane (G14) emerged as the most promising, displaying acceptable performance under both non-stress and salt-stress conditions. These genotypes could serve as valuable genetic resources for breeding programs aimed at enhancing wheat’s salinity tolerance, particularly in arid and semi-arid regions.

Abiotic stresses pose significant challenges to wheat farming, yet exploiting the genetic variability within germplasm collections offers an opportunity to effectively address these challenges. In this study, we investigated the genetic diversity of key agronomic traits among twenty durum wheat cultivars, with the intention to pinpoint those better suited to semi-arid conditions. Field trials were conducted at the ITGC-FDPS Institute, Setif, Algeria, during the winter season of 2021/22. A completely randomized design was used with three replicates. Statistical analyses revealed significant variation among the genotypes for most of the studied traits, with some cultivars exhibiting a superior performance in a stressful environment. Notably, traits like the number of grains per spike (NGS) and the grain yield (GY) displayed high genotypic coefficients of variation (CVg). Except for membrane thermostability (MT) and biological yield (BY), the majority of the assessed traits exhibited moderate-to-high heritability estimates. Genotypic and phenotypic correlation studies have confirmed the importance of many yield-related traits in the expression of GY. The harvest index (HI) underscored the highest genotypic direct effect on GY, followed closely by spike number (SN), serving as consistent pathways through which most of the measured traits indirectly influenced GY. The cluster analysis categorized the durum wheat cultivars into seven distinct clusters. The largest inter-cluster distance was observed between clusters G3 and G4 (D² = 6145.86), reflecting maximum dissimilarity between the individuals of these clusters. Hybridizing divergent clusters may benefit future breeding programs aiming to develop potential durum wheat varieties through cross combinations. This study’s findings contribute to sustainable agriculture efforts by facilitating the selection of genotypes with enhanced resilience and productivity, particularly for cultivation in challenging semi-arid regions.