The roots and rhizomes of Rubia tinctorum L. have been widely used both for industrial and medicinal purposes for centuries. The primary biologically active substances from Rubia tinctorum L. roots and rhizomes are anthraquinone derivatives such as ruberythric acid and lucidin-3-primeveroside. Their identification and quantification are carried out by various analytical methods, requiring a complicated sample preparation as well as special reagents and reference samples. However, NMR spectroscopy has no limitations of this kind. In this work, we have developed and validated a new express and standard-free method for the qualitative and quantitative analysis of ruberythric acid and lucidin-3-primeveroside by ¹H NMR spectroscopy in the extracts from the roots and rhizomes of Rubia tinctorum L. In this work, we have optimized the conditions of the sample preparation and registration of ¹H NMR spectra, determined the optimal solvent and reference compound and confirmed the obtained results by HPLC-UV-MS.

Разработка и валидация методики количественного определения остаточных органических растворителей в препаратах аллергенов методом ГЖХ

To study the influence of ecological growing conditions on the chemical composition of plants, supercritical carbon dioxide extraction is carried out of wild carrot samples, widespread in all regions of the world with a temperate climate (Daucus carota subsp. maritimus). Using the methods of gas-liquid chromatography and mass spectral analysis, volatile organic compounds, fatty acids, and phytosterols in the studied objects are determined in the extracts obtained. The dependence of the chemical composition of wild carrots on its growing conditions (temperature, precipitation, solar radiation, etc.) is shown.

The lipid complex of the aboveground part of Portulaca oleracea L., which constitutes around 2.0% of the plant material, was thoroughly analyzed using a combination of advanced techniques including GC-MS, GC-FID and 1H NMR spectroscopy. The study delved into the qualitative and quantitative composition of various components such as fatty acids, tocopherols, sterols, and triterpene alcohols present in the lipid complex. The primary structural element identified in the complex is wax, with prominent percentages of α-linolenic (23.5%), linoleic (24.7%), and palmitic (25.3%) acids. The tocopherol content in the lipid complex was found to be approximately 0.02%, with β- and γ-tocopherols making up to 40% of the fraction. Additionally, δ-tocopherol and α-tocopherol were present in roughly equal amounts, each constituting about 30% of the tocopherol content. The lipid complex also contains sterols and triterpene alcohols, with a total content of 691.3 mg/100g (0.7%) in the fraction. The main components of this fraction included β-sitosterol (44.0%), Δ7-sitosterol (13.2%), and cycloartenol (7.7%). These detailed analyses highlight the potential of Portulaca oleracea L. as a promising raw material for the extraction of a lipid complex, offering opportunities for further pharmacotherapeutic research and applications.

We studied a possibility of using direct analysis in real time (DART) mass spectrometry in combination with in situ derivatization by nitrogenous heterocyclic compounds for the rapid profiling of sterols in sweetened condensed milk. The data obtained are consistent with the detection of plant sterols by gas chromatography–mass spectrometry (GC–MS). The proposed approach can be used to detect vegetable fats in condensed milk, and the time required for the analysis of one sample, including sample preparation, is several times shorter than that in GC–MS.

Изучена возможность применения масс-спектрометрии с «прямым анализом в реальном времени» (DART) в сочетании с in situ дериватизацией азотистыми гетероциклическими соединениями для экспрессного профилирования стеринов в сгущенном молоке. Показано, что получаемые этим способом данные соответствуют результатам применения методик обнаружения растительных стеринов с помощью газовой хроматографии/масс-спектрометрии (ГХ/МС). Таким образом, предложенный подход может быть использован для установления факта добавки растительных жиров в сгущенное молоко, причем необходимое время на анализ одной пробы с учетом пробоподготовки по сравнению с ГХ/МС сокращается в несколько раз. The possibility of using mass spectrometry with "direct analysis in real time" (DART) in combination with in-situ derivatization by nitrogenous heterocyclic compounds for the rapid profiling of sterols in sweetened condensed milk was studied. The data obtained by the method were shown to correspond to the results of detection of plant sterols using gas chromatography / mass spectrometry (GC/MS). Thus, the proposed approach can be used to reveal adding vegetable fats to curdled milk, and the time required for the analysis of one sample including sample preparation is reduced in several times as compared to GC/MS.