The occurrence of Listeria monocytogenes in marine fish and fish market areas was investigated. Two hundred and eighty-eight samples (123 environmental samples—siphons, knives, cutting boards, floor, sinks, water, and ice—and 165 marine fish samples) were examined. Twenty-four isolates were characterized as Listeria monocytogenes (five from environmental samples (4.0%) and 19 from fish samples (11.5%)). The strains were further characterized according to their antibiotic resistance, pathogenicity, and biofilm formation ability. They were molecularly serotyped as IIc (n = 22) and IVb (n = 2) and possessed all the virulence genes tested (inlA, inlB, inlC, inlJ, actA, hlyA, iap, plcA, and prfA), except for two strains lacking the hlyA and iap genes, respectively. All strains showed strong (41.7%) or moderate biofilm-producing ability (58.3%) and almost all showed resistance to at least one antibiotic, with the highest rates being observed against clindamycin and vancomycin. The proteomic analysis by MALDI-TOF revealed two distinct clusters that involved strains from fish only and those from both fish and the environment. The presence of Listeria monocytogenes in the fish-market environment and marine fish, along with the pathogenicity and persistence characteristics of the seafood-related strains, emphasize the need for vigilance concerning the spread of this notorious foodborne pathogen.

Listeria monocytogenes, the leading cause of fatalities among foodborne pathogens, exhibits remarkable resilience in food industry environments. This study aims to isolate and characterize L. monocytogenes strains along the beef production chain of a vertically integrated industry. Sampling included carcass, hide, and environmental samples collected from a cattle unit and partner slaughterhouse. The samples were examined for the presence of Listeria monocytogenes. The isolates were characterized via molecular serotyping and MALDI-TOF proteomic analysis. Further analyses included antimicrobial susceptibility testing, biofilm formation assessment, and virulence gene investigation. A total of 24 L. monocytogenes strains were isolated. The prevalence in carcasses, hide and slaughterhouse environment was 21.7%, 6.7% and 8.8% respectively. Molecular serogroup IIc predominated, followed by serogroups IIa, IIb, and IVb. Almost all strains showed a moderate or strong ability to produce biofilms and resistance to at least one antimicrobial, with clindamycin, vancomycin, and tetracycline demonstrating the highest rates of resistance. Three strains were also characterized as multidrug-resistant, while all strains possessed at least six virulence genes. The proteomic analysis organized the isolated strains in three clusters, whereas the transmission pathways primarily implicated carcass washing water and hides. Understanding L.monocytogenes dissemination along the beef production chain is vital for preventing transmission to humans and emergence of a listeriosis outbreak.