A glucose biosensor was utilized as a platform for the time-temperature integrator (TTI), a device for intelligent food packaging. The TTI system is composed of glucose oxidase, glucose, a pH indicator, and a three-electrode potentiostat, which produces an electrical signal as well as color development. The reaction kinetics of these response variables were analyzed under isothermal conditions. The reaction rates of the electrical current and color changes were 0.0360 ± 0.0020 (95% confidence limit), 0.0566 ± 0.0026, 0.0716 ± 0.0024, 0.1073 ± 0.0028 µA/min, and 0.0187 ± 0.0005, 0.0293 ± 0.0018, 0.0363 ± 0.0012, 0.0540 ± 0.0019 1/min, at 5, 15, 25, and 35 °C, respectively. The Arrhenius activation energy of the current reaction (Ea_current) was 25.0 ± 1.6 kJ/mol and the Ea_color of the color reactions was 24.2 ± 0.6 kJ/mol. The similarity of these Ea shows agreement in the prediction of food qualities between the electrical signal and color development. Consequently, the function of the new time-temperature integrator system could be extended to that of a biosensor compatible with any electrical utilization equipment.

Antimicrobials have been used to improve animal welfare, food security, and food safety that promote the emergence, selection, and dissemination of antimicrobial-resistant (AMR) bacteria. In this study, 50 E. coli were isolated from frozen chicken meat samples in Dhaka city. Antibiotic sensitivity patterns were assessed through the disk diffusion method and finally screened for the presence of antimicrobial resistance genes (ARG) using the polymerase chain reaction (PCR). Among the 160 samples, the prevalence of E. coli was observed in fifty samples (31.25%). All of these isolates were found resistant to at least one antimicrobial agent, and 52.0% of the isolates were resistant against 4–7 different antimicrobials. High resistance was shown to tetracycline (66.0%), followed by resistance to erythromycin (42.0%), ampicillin and streptomycin (38.0%), and sulfonamide (28.0%). In addition, the most prevalent ARGs were tet(A) (66.0%), ereA (64.0%), tet(B) (60.0%), aadA1 and sulI (56.0%), blaCITM (48.0%) and blaSHV (40.0%). About 90.0% of isolates were multidrug resistant. This study reveals for the first time the current situation of E. coli AMR in broilers, which is helpful for the clinical control of disease as well as for the development of policies and guidelines to reduce AMR in broilers production in Bangladesh.

This study was conducted to evaluate the synergistic effects of the mixtures of green silver nanoparticles (AgNPs) with ampicillin and metformin separately in obviating the global threats of antimicrobial resistance and diabetes mellitus. AgNPs was synthesized using Azadirachta indica aqueous leaf extract at varying extract concentration and incubation time. The synthesis of AgNPs in aqueous solution was confirmed by the visual color change and by using a UV-Vis spectrophotometer. The biosynthesized AgNPs was further characterized using Scanning Electron Microscope (SEM) analysis. The experimental results revealed that the biosynthesized AgNPs were polydispersed, smaller in size and aggregated. The mean diameter of the formed AgNPs was 50 nm as evident from the SEM analysis. Additionally, the mixtures of AgNPs with ampicillin and metformin exhibited synergistic in vitro antimicrobial and in vivo antidiabetic effects i.e., being more efficacious than AgNPs, ampicillin and metformin alone. J. of Sci. and Tech. Res. 4(1): 91-100, 2022