With the eddy covariance (EC) technique, net fluxes of carbon dioxide (CO₂) and other trace gases as well as water and energy fluxes can be measured at the ecosystem level. These flux measurements are a main source for understanding biosphere–atmosphere interactions and feedbacks through cross-site analysis, model–data integration, and upscaling. The raw fluxes measured with the EC technique require extensive and laborious data processing. While there are standard tools¹ available in an open-source environment for processing high-frequency (10 or 20 Hz) data into half-hourly quality-checked fluxes, there is a need for more usable and extensible tools for the subsequent post-processing steps. We tackled this need by developing the REddyProc package in the cross-platform language R that provides standard CO₂-focused post-processing routines for reading (half-)hourly data from different formats, estimating the u_* threshold, as well as gap-filling, flux-partitioning, and visualizing the results. In addition to basic processing, the functions are extensible and allow easier integration in extended analysis than current tools. New features include cross-year processing and a better treatment of uncertainties. A comparison of REddyProc routines with other state-of-the-art tools resulted in no significant differences in monthly and annual fluxes across sites. Lower uncertainty estimates of both u_* and resulting gap-filled fluxes by 50 % with the presented tool were achieved by an improved treatment of seasons during the bootstrap analysis. Higher estimates of uncertainty in daytime partitioning (about twice as high) resulted from a better accounting for the uncertainty in estimates of temperature sensitivity of respiration. The provided routines can be easily installed, configured, and used. Hence, the eddy covariance community will benefit from the REddyProc package, allowing easier integration of standard post-processing with extended analysis. ¹http://fluxnet.fluxdata.org/2017/10/10/toolbox-a-rolling-list-of-softwarepackages-for-flux-related-data-processing/, last access: 17 August 2018

Extreme climatic events, such as droughts and heat stress, induce anomalies in ecosystem–atmosphere CO₂ fluxes, such as gross primary production (GPP) and ecosystem respiration (R_eco), and, hence, can change the net ecosystem carbon balance. However, despite our increasing understanding of the underlying mechanisms, the magnitudes of the impacts of different types of extremes on GPP and R_eco within and between ecosystems remain poorly predicted. Here we aim to identify the major factors controlling the amplitude of extreme-event impacts on GPP, R_eco, and the resulting net ecosystem production (NEP). We focus on the impacts of heat and drought and their combination. We identified hydrometeorological extreme events in consistently downscaled water availability and temperature measurements over a 30-year time period. We then used FLUXNET eddy covariance flux measurements to estimate the CO₂ flux anomalies during these extreme events across dominant vegetation types and climate zones. Overall, our results indicate that short-term heat extremes increased respiration more strongly than they downregulated GPP, resulting in a moderate reduction in the ecosystem's carbon sink potential. In the absence of heat stress, droughts tended to have smaller and similarly dampening effects on both GPP and R_eco and, hence, often resulted in neutral NEP responses. The combination of drought and heat typically led to a strong decrease in GPP, whereas heat and drought impacts on respiration partially offset each other. Taken together, compound heat and drought events led to the strongest C sink reduction compared to any single-factor extreme. A key insight of this paper, however, is that duration matters most: for heat stress during droughts, the magnitude of impacts systematically increased with duration, whereas under heat stress without drought, the response of R_eco over time turned from an initial increase to a downregulation after about 2 weeks. This confirms earlier theories that not only the magnitude but also the duration of an extreme event determines its impact. Our study corroborates the results of several local site-level case studies but as a novelty generalizes these findings on the global scale. Specifically, we find that the different response functions of the two antipodal land–atmosphere fluxes GPP and R_eco can also result in increasing NEP during certain extreme conditions. Apparently counterintuitive findings of this kind bear great potential for scrutinizing the mechanisms implemented in state-of-the-art terrestrial biosphere models and provide a benchmark for future model development and testing.

Grasslands play important roles in ecosystem production and support a large farming and grazing industry. An accurate and efficient way is needed to estimate grassland health and production for monitoring and adjusting management to get sustainable products and other ecosystem services. Previous studies of grasslands have shown varying relationships between productivity and biodiversity, with most showing either a positive or a hump-shaped relationship where productivity peaks at intermediate diversity. In this study, we used airborne imaging spectrometry combined with ground sampling and eddy covariance measurements to estimate the spatial pattern of production and biodiversity for two sites of contrasting productivity in a southern Alberta prairie ecosystem. Resulting patterns revealed that more diverse sites generally had greater productivity, supporting the hypothesis of a positive relationship between production and biodiversity for this site. We showed that the addition of evenness to richness (using the Shannon Index of dominant species instead of the number of dominant species alone) improved the correlation with optical diversity, an optically derived metric of biodiversity based on the coefficient of variation in spectral reflectance across space. Similarly, the Shannon Index was better correlated with productivity (estimated via NDVI (Normalized Difference Vegetation Index)) than the number of dominant species alone. Optical diversity provided a potent proxy for other more traditional biodiversity metrics (richness and Shannon index). Coupling field measurements and imaging spectrometry provides a method for assessing grassland productivity and biodiversity at a larger scale than can be sampled from the ground, and allows the integrated analysis of the productivity–biodiversity relationship over large areas.

Advances in Machine Learning and availability of state-of-the-art computational resources, along with digitized healthcare data, have set the stage for extensive application of artificial intelligence in the realm of diagnosis, prognosis, clinical decision support, personalized treatment options, drug development, and the field of biomedicine. Here, we discuss the application of Machine Learning algorithms in patient healthcare and dermatological domains along with the ethical complexities that are involved. In scientific studies, ethical challenges were initially not addressed proportionally (as assessed by keyword counts in PubMed) and just more recently (since 2016) this has started to improve. Few pioneering countries have created regulatory guidelines around how to respect matters of (1) privacy, (2) fairness, (3) accountability, (4) transparency and (5) conflict of interest when developing novel medical Machine Learning applications. While there is a strong promise of emerging medical applications to ultimately benefit both the patients and the medical practitioners, it is important to raise awareness on the five key ethical issues and incorporate them into medical practice in the near future.