The second ExoMars mission will be launched in 2020 to target an ancient location interpreted to have strong potential for past habitability and for preserving physical and chemical biosignatures (as well as abiotic/prebiotic organics). The mission will deliver a lander with instruments for atmospheric and geophysical investigations and a rover tasked with searching for signs of extinct life. The ExoMars rover will be equipped with a drill to collect material from outcrops and at depth down to 2 m. This subsurface sampling capability will provide the best chance yet to gain access to chemical biosignatures. Using the powerful Pasteur payload instruments, the ExoMars science team will conduct a holistic search for traces of life and seek corroborating geological context information. Key Words: Biosignatures—ExoMars—Landing sites—Mars rover—Search for life. Astrobiology 17, 471–510. 1. Article Organization 2. Introduction 2.1. ExoMars origin 2.2. A difficult adolescence 2.3. Joint program 3. Early Mars as an Exobiology Target 3.1. A first window of opportunity for life 3.2. Separate ways 3.2.1. Young Earth 3.2.2. Young Mars 3.2.3. Young Venus 3.3. Lessons for ExoMars: when and where? 4. Biosignatures: Which and How Reliable? 4.1. Morphological biosignatures 4.2. Chemical biosignatures 4.2.1. Isomerism selectivity 4.2.2. Molecular weight fingerprints 4.2.3. Bulk isotopic fractionation 4.3. Importance of geological context for boosting biosignature confidence 4.4. Life's decision points 4.5. Examples using the ExoMars biosignature score 4.5.1. Kitty's Gap, N.W. Australia 4.5.2. Josefsdal Chert, Barberton, South Africa 4.5.3. Martian Meteorite ALH84001 4.5.4. Yellowknife Bay, Mars 5. The Martian Environment and the Need for Subsurface Exploration 5.1. Results from previous missions 5.2. Degradation of organic matter 5.3. Access to molecular biosignatures 6. The ExoMars Rover and Its Pasteur Payload 6.1. From panoramic to molecular scale through nested investigations 6.2. Pasteur payload instruments 6.2.1. Panoramic camera system 6.2.2. IR spectrometer 6.2.3. Shallow ground-penetrating radar 6.2.4. Subsurface neutron detector 6.2.5. Close-up imager 6.2.6. Drill IR spectrometer 6.2.7. Subsurface drill 6.2.8. Sample preparation and distribution system 6.2.9. MicrOmega 6.2.10. Raman laser spectrometer 6.2.11. Mars organic molecule analyzer 6.3. The reference surface mission 7. A Suitable Landing Site 7.1. Scientific constraints 7.2. Engineering constraints 7.3. Planetary protection constraints 7.4. Possible locations for landing 7.4.1. Oxia Planum (18.159°N, 335.666°E; −3 km MOLA) 7.4.2. Mawrth Vallis (22.160°N, 342.050°E; −2 km MOLA) 8. Conclusions Acknowledgments Author Disclosure Statement References Abbreviations Used

We have synthesized inorganic micron-sized filaments, whose microstucture consists of silica-coated nanometer-sized carbonate crystals, arranged with strong orientational order. They exhibit noncrystallographic, curved, helical morphologies, reminiscent of biological forms. The filaments are similar to supposed cyanobacterial microfossils from the Precambrian Warrawoona chert formation in Western Australia, reputed to be the oldest terrestrial microfossils. Simple organic hydrocarbons, whose sources may also be abiotic and indeed inorganic, readily condense onto these filaments and subsequently polymerize under gentle heating to yield kerogenous products. Our results demonstrate that abiotic and morphologically complex microstructures that are identical to currently accepted biogenic materials can be synthesized inorganically.

Roundabout Gypsum: Calcium sulfates are a common but perhaps underappreciated group of minerals used in a number of natural and industrial processes. In many ways, these crystals precipitate from solution in the same way that most other aqueous minerals form; however, mounting evidence suggests that different, unexplored mechanisms may be at work. Van Driessche et al. (p. 69 ; see the cover) performed high-resolution microscopy of the most common calcium sulfate mineral, gypsum, at various points along time-resolved, fast-quenching growth experiments. The images reveal that gypsum particles actually start out as crystalline nanoparticles of another mineral, bassanite, which then self-assemble into well-ordered nanorods. Finally, the nanorods transform into gypsum following a hydration reaction. The observation that the reaction pathway occurs below the solubility limit of the intermediate phase has wide-ranging implications for biomineralization processes and may provide ways to prevent fouling on the surfaces of desalination membranes.

The precipitation of barium or strontium carbonates in alkaline silica-rich environments leads to crystalline aggregates that have been named silica/carbonate biomorphs because their morphology resembles that of primitive organisms. These aggregates are self-assembled materials of purely inorganic origin, with an amorphous phase of silica intimately intertwined with a carbonate nanocrystalline phase. We propose a mechanism that explains all the morphologies described for biomorphs. Chemically coupled coprecipitation of carbonate and silica leads to fibrillation of the growing front and to laminar structures that experience curling at their growing rim. These curls propagate in a surflike way along the rim of the laminae. We show that all observed morphologies with smoothly varying positive or negative Gaussian curvatures can be explained by the combined growth of counterpropagating curls and growing laminae.