Malnutrition is one of the major factors of bone and cartilage disorders. Pacific cod (Gadus macrocephalus) processing waste is a cheap and highly promising source of bioactive substances, including collagen-derived peptides and amino acids, for bone and cartilage structure stabilization. The addition of these substances to a functional drink is one of the ways to achieve their fast intestinal absorption. Collagen hydrolysate was obtained via enzymatic hydrolysis, ultrafiltration, freeze-drying, and grinding to powder. The lyophilized hydrolysate was a light gray powder with high protein content (>90%), including collagen (about 85% of total protein) and a complete set of essential and non-essential amino acids. The hydrolysate had no observed adverse effect on human mesenchymal stem cell morphology, viability, or proliferation. The hydrolysate was applicable as a protein food supply or a structure-forming food component due to the presence of collagen fiber fragments. An isotonic fitness drink (osmolality 298.1 ± 2.1 mOsm/L) containing hydrolysate and vitamin C as a cofactor in collagen biosynthesis was prepared. The addition of the hydrolysate did not adversely affect its organoleptic parameters. The production of such functional foods and drinks is one of the beneficial ways of fish processing waste utilization.

This study is devoted to the investigation of chitinolytic enzymes from the hepatopancreas of two commercially exploited crabs in the North-West region: the Red King crab (Paralitho- des camtschaticus) and the snow crab (Chionoecetes opilio). The results of the study demonstrate the presence of endo- and exochitinase activity in enzyme preparations, allowing for various types of chitin biotransformation. Using these enzymes, it is possible to depolymerize chitin into low-molecular-weight chitin and chitin oligomers, as well as to obtain the monomer N-acetylglucosamine. The authors have shown that crab hepatopancreas can be used as a raw material for obtaining chitinolytic enzymes, which allow for the enzymatic transformation of natural chitin into low-molecular-weight oligosaccharides and N-acetylglucosamine.

It is well known that fish belonging to the Salmonidae family differ in their nutritional value. Anatomical and morphological features of different salmon species have a certain similarity; therefore, representatives of this family are most often falsified. Assortment falsification of products from fish of this family is usually carried out by replacing more valuable species with cheaper ones with a reduced nutritional value. Most often, counterfeiting of Atlantic salmon (salmon) by Far Eastern ones (chum salmon, pink salmon, chinook salmon, coho salmon) is found. Near infrared spectroscopy (NIR) is now increasingly used for identification and authentication of closely related organisms, in some cases being a rapid method replacing genetic analysis. We have obtained diffusion reflectance spectra of NIR radiation for three species of fish from the Northern Basin belonging to the salmon family. The best classification by fish species has been obtained by analyzing the NIR spectra of pre-dried fat-free muscle tissue samples. In case of wet samples, the observed differences are less significant, up to insignificant differences in individual values from neighboring clusters. The possibility of using the method of linear discriminant analysis of the NIR reflection spectra of muscle proteins for the species identification of fish has been shown.