The adaptive potential of plants in urban environments, responding to factors like air pollution, electromagnetic radiation, and specific microclimates, remains insufficiently understood. Our study focused on two evergreen Cupressaceae family species, Thuja occidentalis L. and Platycladus orientalis L. Franco, which are commonly found in Kazakhstan’s urban landscapes. Conducted in Almaty, one of Kazakhstan’s most polluted cities, our comparative analysis examined the anatomical features, photosynthetic activity, and secondary metabolite composition of these conifers. Both species exhibited xeromorphic traits, such as submerged stomata, resin passages, and a prominent leaf cuticle. T. occidentalis displayed higher photosynthetic activity values (quantum yield of photosystem II (YII), electron transport rate (ETR), and quantum yield of non-photochemical quenching (Y(NPQ))) than P. orientalis, while P. orientalis exhibited a higher quantum yield of non-regulated energy dissipation in PSII (Y(NO)) values. Chemical analysis revealed 31 components in T. occidentalis and 33 in P. orientalis, with T. occidentalis containing three times more thujone (16.42% and 5.18%, respectively) and a higher monosaccharide content (17.33% and 6.98%, respectively). T. occidentalis also contained 14.53% steroids, whereas P. orientalis showed no steroid presence. The cytotoxic activity of essential oils was determined by the survival of Artemia salina aquatic crustaceans, whereas tested essential oils from both species exhibited acute lethal toxicity to A. salina aquatic crustaceans across all tested concentrations. The connection between physiological traits, adaptation strategies, and cytotoxic effects offers a comprehensive view of the ecological and pharmacological importance of these two observed conifer species, highlighting their diverse roles in urban environments, as well as their potential medical uses.

Perennial wheat yields for several years after sowing, and develops a deep and dense root system, thereby improving soil structure and preserving soil fertility. It is also drought resistant, which increases its future potential under the ongoing climate change. Moreover, perennial wheat offers and effective strategy for carbon sequestration through increasing soil carbon stocks. However, the effect of perennial wheat on soil properties remains uncertain in the conditions of south-eastern Kazakhstan due to the lack of data. To fill this gap, we conducted a study in which five varieties of perennial wheat were grown in the Almaty region in soils of different fertility for two years. As a result, in the plot where topsoil was removed to a depth of 25 cm, total carbon increased by nearly three times, from 0.54% to 1.42%, and the number of microorganisms increased from 715.1x103±0.2x103 to 48.0x103±0.6x103 colony forming units per ml of soil. The photosynthetic performance of perennial wheat varieties in the experimental plot was not negatively affected by the removal of top soil suggesting that perennial wheat can successfully restore soil fertility and replenish soil with chemical elements involved in photosynthesis. Our results confirm that in the conditions of the south-eastern Kazakhstan perennial wheat makes a significant contribution to the soil carbon stock accumulation and development of soil biota; in our study, these effects were particularly strong in the case of degraded soil.