Drought and salinity are considered to be the major limiting factors for plant growth in desert areas. Halophytes can maintain their growth in extreme salt environments. However, few studies have linked the photosynthetic characteristics of halophytes with their anatomical structures and leaf water potential to jointly explain the effects of drought and salt on leaf internal water use efficiency (WUEi). For this reason, two different halophytes, Nitraria sibirica and Alhagi sparsifolia, were selected to analyze the leaf physiological traits in response to different water and salt gradients. The analysis further revealed the influence of soil water and salt content and anatomical characteristics on plant photosynthesis. The results showed that the leaf water potential, morphology, anatomical parameters, and photosynthetic parameters of the two plants in different habitats showed significant species-specific physiological responses. The value of WUEi of N. sibirica was negatively correlated with net photosynthetic rate (Pn), stomatal conductance (Gs), tightness of palisade tissue (CTR), and soil factors, while the WUEi of A. sparsifolia was positively correlated with Pn, Gs, and CTR. The indirect effects of soil salinity and soil moisture on WUEi were also found to be species-specific. This study will help to improve the understanding of the ecological adaptability of plants to water and salt stress and provide a theoretical basis for clarifying the drought tolerance and salt tolerance adaptation strategies of halophytes in arid areas.

Cow dung is a kind of high quality and renewable biological resource. Biochar made from cow dung can be used as a soil amendment to improve soil nutrient status. The relationship between soil water and phosphorus is very close, and the water status determines the form, content, and availability of phosphorus. In order to investigate the effects of biochar on soil inorganic phosphorus components, available phosphorus, enzyme activities related to the phosphorus cycle, microbial functional genes, and seedling growth under different soil water conditions were investigated. Field experiments were carried out by setting different water conditions (30%, 60%, and 100%) and biochar addition (0 t hm⁻², 2.63 t hm⁻², 5.26 t hm⁻², and 7.89 t hm⁻²). The results showed that applying biochar significantly increased the soil’s accessible phosphorus content and the phosphorus content in both the aboveground and subsurface parts of P. euphratica seedlings. This is mainly attributable to biochar’s direct and indirect effects on soil properties. Because biochar is naturally alkaline, it raises soil pH and reduces acid phosphatase activity in the soil around P. euphratica seedlings in the rhizosphere. Perhaps the alkaline phosphatase level first showed an upward trend due to the combined impacts of water and biochar, and then it started to decline when the biochar addition was increased. Soil phosphorus functional genes phoC, phoD, gcd, and pqqc had an increase in copy number with biochar addition but not without treatment. Indirectly, the biochar treatment increased the soil’s phosphorus availability by increasing the population of the phosphate-solubilizing bacteria Fusarium and Sphingomonas. Soil phosphorus availability is positively affected by biochar under various water conditions. This impact is due to chemical and microbiological mechanisms.