The Jinchanghe Pb-Zn polymetallic deposit is a representative distal skarn deposit in the Baoshan block. Due to limited research on the sources of ore-forming metals and trace elemental geochemistry of sulfides, the sources and mineralization environments of the Jinchanghe skarn Pb-Zn polymetallic deposit are still controversial. In this study, copper and sulfur isotopes and trace elements of sulfides from the Jinchanghe Pb-Zn polymetallic deposit were analyzed to reveal the sources of ore-forming materials and the ore-forming environments. The trace elemental analysis results show abundant Co, Ni, Mn and Se elements, and less As, Tl and Sb elements dominantly enter pyrite by isomorphic substitution, except for some Zn and Cu that occur as sulfide inclusions in Py1. Abundant Fe and Mn elements could substitute Zn to enter the sphalerite crystal lattice by the isomorphic substitution mechanism of (Fe²⁺ + Mn²⁺) ↔ 2Zn²⁺, and less Ag and Sb elements could enter the sphalerite by the isomorphic substitution mechanism of (Ag⁺ + Sb³⁺) ↔ 2Zn²⁺. The Cu-S isotopic and trace elemental compositions jointly suggest that the Jinchanghe distal skarn Pb-Zn polymetallic deposit formed from magmatic hydrothermal fluids with a sedimentary host-rock contribution, under mesothermal conditions (260 °C on sphalerite).