Nitrification inhibitors are essential in agricultural and environmental production practices. They play a crucial role in promoting agricultural and environmental sustainability by enhancing nitrogen use efficiency, boosting crop yields, and mitigating the adverse environmental effects of nitrogen losses. This bibliometric analysis covers the period from 2004 to 2023, offering a detailed examination of the development of nitrification inhibitor research. The study demonstrates a consistent growth in research publications, indicating sustained interest and dedication to advancing the field. It identifies key contributors, such as institutions and researchers, and underscores the significance of their work through citation analysis. Keyword co-occurrence analysis reveals four distinct clusters focusing on enhancing crop yields, understanding microbial community dynamics, exploring grazing pasture applications, and addressing environmental impact mitigation. The cutting-edge area of keyword burst detection research has transitioned from fundamental research to comprehensive nitrogen management practices. This analysis provides insights into the current research landscape of nitrification inhibitors and proposes future research directions, underscoring the critical role of this field in tackling global agricultural and environmental challenges.

Shouchella clausii (S. clausii) has been marketed as an important commercial probiotic, displaying significant therapeutic effects on antibiotic-associated diarrhea and providing benefits to humans. This study aimed to explore the distribution, adaptation, and probiotic properties of S. clausii. Based on 16S rRNA gene analysis, 43 strains of S. clausii were isolated from 317 soil samples in China. Based on the genomic index of Average Nucleotide Identity (ANI) results, 41 strains were confirmed as S. clausii, while two strains, FJAT-45399 and FJAT-45335, were identified as potential novel species distinct from S. clausii. Combined phenotypic and genomic predictions indicated that S. clausii could survive under harsh conditions. Comparative genomics revealed that these isolates possess antibiotic resistance genes, as well as capabilities for bacteriocin and folate production, while lacking toxins and hemolytic activity. Hemolysis tests indicated that strain FJAT-41761 exhibited non-pathogenic γ-hemolytic activity, while also demonstrating resistance to multiple antibiotics, consistent with probiotic characteristics. These findings suggest that strain FJAT-41761 is safe and holds potential as a future probiotic.