The 14-3-3 protein may regulate many biochemical and physiological processes under abiotic stress. Studies have shown that the 14-3-3 protein gene of AMF is not only upregulated under drought stress, but also enhances the regulation of AMF on plant drought tolerance by regulating plant signal pathways and drought response genes; however, knowledge about the biological relevance of these interactions remains limited and controversial.

Casuarina glauca is an important coastal protection forest species, which is exposed to high salt stress all year round. Arbuscular mycorrhizal fungi (AMF) can promote the growth and salt tolerance of C. glauca under salt stress. However, the effects of AMF on the distribution of Na⁺ and Cl⁻ and the expression of related genes in C. glauca under salt stress need to be further explored. This study explored the effects of Rhizophagus irregularis on plant biomass, the distribution of Na⁺ and Cl⁻, and the expression of related genes in C. glauca under NaCl stress through pot simulation experiments. The results revealed that the mechanisms of Na⁺ and Cl⁻ transport of C. glauca under NaCl stress were different. C. glauca took a salt accumulation approach to Na⁺, transferring Na⁺ from roots to shoots. Salt accumulation of Na⁺ promoted by AMF was associated with CgNHX7. The transport mechanism of C. glauca to Cl⁻ might involve salt exclusion rather than salt accumulation, and Cl⁻ was no longer transferred to shoots in large quantities but started to accumulate in roots. However, AMF alleviated Na⁺ and Cl⁻ stress by similar mechanisms. AMF could promote salt dilution of C. glauca by increasing biomass and the content of K⁺, compartmentalizing Na⁺ and Cl⁻ in vacuoles. These processes were associated with the expression of CgNHX1, CgNHX2-1, CgCLCD, CgCLCF, and CgCLCG. Our study will provide a theoretical basis for the application of AMF to improve salt tolerance in plants.

Introduction: Saline-alkali stress seriously endangers the normal growth of Populus simonii×P. nigra. Arbuscular mycorrhizal (AM) fungi can enhance the saline-alkali tolerance of plants by establishing a symbiotic relationship with them.Methods: In this study, a pot experiment was conducted to simulate a saline-alkali environment where Populus simonii×P. nigra were inoculated with Funneliformis mosseae to explore their effects on the saline-alkali tolerance of Populus simonii×P. nigra.Results and Discussion: Our results show that a total of 8 NHX gene family members are identified in Populus simonii×P. nigra. F. mosseae regulate the distribution of Na+ by inducing the expression of PxNHXs. The pH value of poplar rhizosphere soil is reduced, result in the promote absorption of Na⁺ by poplar, that ultimately improved the soil environment. Under saline-alkali stress, F. mosseae improve the chlorophyll fluorescence and photosynthetic parameters of poplar, promote the absorption of water, K⁺ and Ca²⁺, thus increase the plant height and fresh weight of aboveground parts, and promote the growth of poplar. Our results provide a theoretical basis for further exploring the application of AM fungi to improve the saline-alkali tolerance of plants.

The application of arbuscular mycorrhizal fungi (AM fungi) and phosphorus (P) can improve plant growth under drought stress by upregulating the antioxidant system and osmotic accumulation. The 14-3-3 protein can respond to different abiotic stresses such as low P and drought. The purpose of this experiment was to study the effects of AM fungi (Rhizophagus intraradices) inoculation on reactive oxygen species (ROS) homeostasis, P metabolism, and 14-3-3 gene expression of Populus cathayana at different P levels and drought stress (WW: well-watered and WD: water deficit). Under WD conditions, AM fungi inoculation significantly increased the P content in leaves and roots, but the benefit in roots is limited by the level of P addition, and the roots may have more alkaline phosphatase and phytase under P stress, and these activities in the rhizosphere soil inoculated with AM fungi were stronger. Under WD conditions, the activities of catalase (leaf and root) and peroxidase (root) inoculated with AM fungi were significantly higher than those without inoculation and decreased with P addition. 14-3-3 genes, PcGRF10 and PcGRF11, have a positive correlation with the antioxidant system, osmotic regulation, and P metabolism, which may be more significant after inoculation with AM fungi. Our results provide new insights into the mechanism of ROS homeostasis and P metabolism in mycorrhizal plants under drought stress.

Adsorption processes at mineral–water interfaces control the fate and transport of arsenic in soils and aquatic systems. Mechanistic and thermodynamic models to describe this phenomenon only consider inner-sphere complexes but recent observation of the simultaneous adsorption of inner- and outer-sphere arsenate on single crystal surfaces complicates this picture. In this study, we investigate the ionic strength-dependence of the macroscopic adsorption behavior and molecular-scale surface speciation of arsenate bound to gibbsite and bayerite. Arsenate adsorption decreases with increasing ionic strength on both minerals, with a larger effect at pH 4 than pH 7. The observed pH-dependence corresponds with a substantial decrease in surface charge at pH 7, as indicated by ζ-potential measurements. Extended X-ray absorption fine structure (EXAFS) spectroscopy finds that the number of second shell Al neighbors around arsenate is lower than that required for arsenate to occur solely as an inner-sphere surface complex. Together, these observations demonstrate that arsenate displays macroscopic and molecular-scale behavior consistent with the co-occurrence of inner- and outer-sphere surface complexes. This demonstrated that outer-sphere species can be responsible for strong adsorption of ions and suggests that environments experiencing an increase in salt content may induce arsenic release to water, especially under weakly acidic conditions.