This page has only limited features, please log in for full access.
In this study, a hydrogel using single and double crosslinking was prepared using GelMA, a natural polymer, and the effect was evaluated when the double crosslinked hydrogel and tannic acid were treated. The resulting hydrogel was subjected to physicochemical property evaluation, biocompatibility evaluation, and animal testing. The free radicals generated through APS/TEMED have a scaffold form with a porous structure in the hydrogel, and have a more stable structure through photo crosslinking. The double crosslinked hydrogel had improved mechanical strength and better results in cell compatibility tests than the single crosslinked group. Moreover, in the hydrogel transplanted into the femur of a rat, the double crosslinked group showed an osteoinductive response due to the attachment of bone minerals after 4 and 8 weeks, but the single crosslinked group did not show an osteoinductive response due to rapid degradation. Treatment with a high concentration of tannic acid showed significantly improved mechanical strength through H-bonding. However, cell adhesion and proliferation were limited compared to the untreated group due to the limitation of water absorption capacity, and no osteoinduction reaction was observed. As a result, it was confirmed that the treatment of high-concentration tannic acid significantly improved mechanical strength, but it was not a suitable method for improving bone induction due to the limitation of water absorption.
Ji-Bong Choi; Yu-Kyoung Kim; Seon-Mi Byeon; Jung-Eun Park; Tae-Sung Bae; Yong-Seok Jang; Min-Ho Lee. Characteristics of Biodegradable Gelatin Methacrylate Hydrogel Designed to Improve Osteoinduction and Effect of Additional Binding of Tannic Acid on Hydrogel. Polymers 2021, 13, 2535 .
AMA StyleJi-Bong Choi, Yu-Kyoung Kim, Seon-Mi Byeon, Jung-Eun Park, Tae-Sung Bae, Yong-Seok Jang, Min-Ho Lee. Characteristics of Biodegradable Gelatin Methacrylate Hydrogel Designed to Improve Osteoinduction and Effect of Additional Binding of Tannic Acid on Hydrogel. Polymers. 2021; 13 (15):2535.
Chicago/Turabian StyleJi-Bong Choi; Yu-Kyoung Kim; Seon-Mi Byeon; Jung-Eun Park; Tae-Sung Bae; Yong-Seok Jang; Min-Ho Lee. 2021. "Characteristics of Biodegradable Gelatin Methacrylate Hydrogel Designed to Improve Osteoinduction and Effect of Additional Binding of Tannic Acid on Hydrogel." Polymers 13, no. 15: 2535.
Gelatin methacryloyl (GelMA) has been widely studied as a biomaterial for tissue engineering. Most studies focus on mammalian gelatin, but certain factors, such as mammalian diseases and diet restrictions, limit the use of mammalian gelatin. Thus, fish gelatin has received much attention as a substitute material in recent years. To develop a broadly applicable hydrogel with excellent properties, an interpenetrating polymer network (IPN) hydrogel was synthesized, since IPN hydrogels consist of at least two different hydrogel components to combine their advantages. In this study, we prepared GelMA using type A and fish gelatin and then synthesized IPN hydrogels using GelMA with alginate. GelMA single-network hydrogels were used as a control group. The favorable mechanical properties of type A and fish hydrogels improved after the synthesis of the IPN hydrogels. Type A and fish IPN hydrogels showed different mechanical properties (mechanical strength, swelling ratio, and degradation rate) and different cross-sectional morphologies, since the degree of mechanical enhancement in fish IPN hydrogels was less than that in type A; however, the cell biocompatibilities were not significantly different. Therefore, these findings could serve as a reference for future studies when selecting GelMA as a biological material for tissue engineering.
Chen Ma; Ji-Bong Choi; Yong-Seok Jang; Seo-Young Kim; Tae-Sung Bae; Yu-Kyoung Kim; Ju-Mi Park; Min-Ho Lee. Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering. ACS Omega 2021, 6, 17433 -17441.
AMA StyleChen Ma, Ji-Bong Choi, Yong-Seok Jang, Seo-Young Kim, Tae-Sung Bae, Yu-Kyoung Kim, Ju-Mi Park, Min-Ho Lee. Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering. ACS Omega. 2021; 6 (27):17433-17441.
Chicago/Turabian StyleChen Ma; Ji-Bong Choi; Yong-Seok Jang; Seo-Young Kim; Tae-Sung Bae; Yu-Kyoung Kim; Ju-Mi Park; Min-Ho Lee. 2021. "Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering." ACS Omega 6, no. 27: 17433-17441.
본 연구의 목적은 다색의 지르코니아 블록과 리튬 디실리케이트 블록의 글레이즈 처리가 광학적 특성에 미치는 영향을 조사하는 것이다. 지르코니아계 50% 입방정 함유 Ultra Translucent Multi-Layered (UTML) disc와 리튬 디실리케이트계 Amber Mill Direct (AMD) block을 크라운의 상층부(T), 중앙부(M), 하층부(B)를 상정하여 최종 두께가 1.2 mm가 되도록 준비하였다. 세 종류의 paste glaze (InSyncⓇ Glaze Paste, IPS E.max Ceram Glaze Paste, Amber Ceram G7 Glaze Paste)를 시편의 표면에 균일한 두께로 적용하고 소성하였다. CIE L*, a*, b* 값은 분광 광도계를 사용하여 각 시편의 상층부, 중앙부 및 하층부에서 각각 3회씩 측정하여 분석하였다. HR FE-SEM을 이용하여 모재와 글레이즈 층과의 결합계면을 관찰하였다. 글레이즈 처리한 실험군에서는 L* 값은 증가하고, a* 값과 b* 값은 감소하였다. 무처리군에서 측정된 반투명도 값은 UTML보다 AMD군에서 통계학적으로 유의하게 더 높았다(P<0.05). 반투명도는 UTML군에서는 글레이즈 처리 전후 유의한 차이를 보이지 않았지만(P>0.05), AMD군의 글레이즈 처리 후 무처리 군에 비해 유의하게 감소되었다(P<0.05). 대조비는 글레이즈 처리 후 무처리 군에 비해 전반적으로 증가한 양상을 보였다. 결론적으로, 글레이즈 처리는 세라믹 수복물 제작의 마지막 단계로서 광학적 특성에 영향을 줄 수 있으므로 치과기공사가 원하는 색조를 재현하고자 할 때 글레이즈의 선택은 중요하게 고려되어야 할 것으로 생각된다.
Byeol Hwang; Jeong-Hui Ji; Cheol-Soo Park; Jaewoo Shin; Junghwan Lee; Min-Ho Lee; Tae-Sung Bae. Color comparison of glaze-treated multi-coloured lithium disilicate and zirconia. Korean Journal of Dental Materials 2021, 48, 1 -14.
AMA StyleByeol Hwang, Jeong-Hui Ji, Cheol-Soo Park, Jaewoo Shin, Junghwan Lee, Min-Ho Lee, Tae-Sung Bae. Color comparison of glaze-treated multi-coloured lithium disilicate and zirconia. Korean Journal of Dental Materials. 2021; 48 (1):1-14.
Chicago/Turabian StyleByeol Hwang; Jeong-Hui Ji; Cheol-Soo Park; Jaewoo Shin; Junghwan Lee; Min-Ho Lee; Tae-Sung Bae. 2021. "Color comparison of glaze-treated multi-coloured lithium disilicate and zirconia." Korean Journal of Dental Materials 48, no. 1: 1-14.
본 연구는 교정용 미니스크류의 구조적 특징인 나사부의 길이 변화와 cutting flute의 형성이 삽입 및 제거 토크에 미치는 영향을 평가하기 위해 시행되었다. 미니스크류는 나사부의 길이가 각각 6.0 mm, 3.3 mm 2종류를 제작하였다. Flute 형성 영향을 살펴보기 위해 flute를 형성하지 않은 미니스크류 실험군과 flute를 형성한 실험군으로 나누었다. Flute 형성 시 미니스크류의 tip으로부터 6.0 mm인 경우는 4.0 mm 그리고 3.3 mm인 경우는 2.4 mm까지 flute를 원주상의 180°위치에 2개를 형성하였다. 또한 flute 형성의 영향을 살펴보기 위해 미니스크류의 tip으로부터 6.0 mm 경우는 4 mm 그리고 3.3 mm 경우는 2.4 mm까지 flute를 원주상의 180°위치에 2개를 형성하였다. 삽입 부위에 따른 피질골의 두께와 골밀도의 차이에 따른 영향을 배제하기 위해 피질골 두께 2 mm와 해면골 두께 10 mm의 인공골 Biomechanical Test Block (Sawbones Inc, Vashon, WA, USA)을 이용하였다. 인공골에 직경 1 mm 드릴로 guide hole을 형성하고 미니스크류를 수직하게 위치시킨 다음 0.1 N·cm 정밀도의 디지털 토크 게이지 (9810P, Aikoh Engineering Co., Japan)에 약 1.2 kg의 정하중을 가한 상태에서 나사부의 top까지 삽입하며 얻어진 최대 토크 값과 삽입 방향의 역방향으로 제거할 때의 최대 토크 값을 기록하였다. 길이 6.0 mm와 3.3 mm 미니스크류의 삽입 토크 값은 각각 (29.53±1.84) N·cm와 (26.84±2.15) N·cm, 그리고 제거 토크 값은 각각 (14.50±1.37) N·cm와 (13.15±2.89) N·cm를 보였으며 상호간에 통계학적으로 유의한 차이를 보이지 않았다(P>0.05). Flute의 형성으로 6.0 ㎜ 미니스크류에서는 삽입과 제거 토크 값이 각각 (30.13±1.97) N·cm와 (18.65±1.10) N·cm로 공히 증가되었는데 통계학적으로 유의한 차이를 보이지는 않았다(P>0.05). 길이 3.3 mm 미니스크류에서는 삽입과 제거 토크 값은 각각 (20.99±3.94) N·cm와 (11.32±2.03) N·cm로 공히 감소되었는데 삽입 토크 값에서만 통계학적으로 유의한 감소를 보였다(P<0.05). 미니스크류의 삽입과 제거 토크 값은 나사부의 길이가 2배 증가되는 경우에도 큰 폭으로 증가되지 않았고, flute 형성의 영향은 나사부의 길이에 따라 다르게 나타났다.
Cheol-Soo Park; Byoung-Gu Lim; Eun-Kyu Won; Jo-Yeon Hwang; Byeol Hwang; Jeong-Hui Ji; Woo-Yong Jeon; Min-Ho Lee; Tae-Sung Bae. Effect of the cutting flute of the orthodontic miniscrew on insertion and removal torque. Korean Journal of Dental Materials 2021, 48, 53 -60.
AMA StyleCheol-Soo Park, Byoung-Gu Lim, Eun-Kyu Won, Jo-Yeon Hwang, Byeol Hwang, Jeong-Hui Ji, Woo-Yong Jeon, Min-Ho Lee, Tae-Sung Bae. Effect of the cutting flute of the orthodontic miniscrew on insertion and removal torque. Korean Journal of Dental Materials. 2021; 48 (1):53-60.
Chicago/Turabian StyleCheol-Soo Park; Byoung-Gu Lim; Eun-Kyu Won; Jo-Yeon Hwang; Byeol Hwang; Jeong-Hui Ji; Woo-Yong Jeon; Min-Ho Lee; Tae-Sung Bae. 2021. "Effect of the cutting flute of the orthodontic miniscrew on insertion and removal torque." Korean Journal of Dental Materials 48, no. 1: 53-60.
In the field of bone tissue, maintaining adequate mechanical strength and tissue volume is an important part. Recently, biphasic calcium phosphate (BCP) was fabricated to solve the shortcomings of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP), and it is widely studied in the field of bone-tissue engineering. In this study, a composite hydrogel was fabricated by applying BCP to gelatin methacrylate (GelMA). It was tested by using a mechanical tester, to characterize the mechanical properties of the prepared composite hydrogel. The fabricated BCP was analyzed through FTIR and XRD. As a result, a different characteristic pattern from hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP) was observed, and it was confirmed that it was successfully bound to the hydrogel. Then, the proliferation and differentiation of preosteoblasts were checked to evaluate cell viability. The analysis results showed high cell viability and relatively high bone differentiation ability in the composite hydrogel to which BCP was applied. These features have been shown to be beneficial for bone regeneration by maintaining the volume and shape of the hydrogel. In addition, hydrogels can be advantageous for clinical use, as they can shape the structure of the material for custom applications.
Ji-Bong Choi; Yu-Kyoung Kim; Seon-Mi Byeon; Jung-Eun Park; Tae-Sung Bae; Yong-Seok Jang; Min-Ho Lee. Fabrication and Characterization of Biodegradable Gelatin Methacrylate/Biphasic Calcium Phosphate Composite Hydrogel for Bone Tissue Engineering. Nanomaterials 2021, 11, 617 .
AMA StyleJi-Bong Choi, Yu-Kyoung Kim, Seon-Mi Byeon, Jung-Eun Park, Tae-Sung Bae, Yong-Seok Jang, Min-Ho Lee. Fabrication and Characterization of Biodegradable Gelatin Methacrylate/Biphasic Calcium Phosphate Composite Hydrogel for Bone Tissue Engineering. Nanomaterials. 2021; 11 (3):617.
Chicago/Turabian StyleJi-Bong Choi; Yu-Kyoung Kim; Seon-Mi Byeon; Jung-Eun Park; Tae-Sung Bae; Yong-Seok Jang; Min-Ho Lee. 2021. "Fabrication and Characterization of Biodegradable Gelatin Methacrylate/Biphasic Calcium Phosphate Composite Hydrogel for Bone Tissue Engineering." Nanomaterials 11, no. 3: 617.
본 연구의 목적은 열가압성형법을 적용하는 과정에서 일어나는 지르코니아와 매몰재 사이의 반응이 지르코니아 강도에 미치는 영향을 평가하는 것이다. Zirtooth™ Multi O-9814 블록 (∅98 × 14T, HASS, Gangwondo, Korea)을 24 mm×4 mm×0.5 mm로 절삭 가공하여 총 60개의 시편을 만든 다음 1450℃ 에서 소결했다. 시편은 매몰재에 따라 6개의 하위 그룹으로 나누었다. (a) UN 그룹 (대조군), (b) PH 그룹 (Prime vest HS), (c) CP 그룹 (Calibra-press), (d) BV 그룹 (BC-Vest), (e) MH 그룹 (Microstar- HS), (f) F1 그룹 (Fomula 1). 5가지 매몰재를 사용하여 제조사가 권장하는 절차에 따라 매몰한 다음 상온에서 30분간 방치했다. 매몰재 주형을 건조하고 소환온도 850℃ 에서 50분간 계류시켰다. 그리고 Amber Lisi-POZ LT (HASS)를 주형과 함께 열가압성형 전기로(Programat EP3000/G2, Ivoclar Vivadent, Schaan, Liechtenstein)에 넣고 상승온도 45℃/min 으로 915℃ 까지 가열한 다음 15분 동안 계류시켰다. 시편은 재료시험기(universal testing machine 4201, instron, Canton, MA, USA)에 준비된 시편을 올려놓고 crosshead speed 0.5mm/min 에서 파절 하중을 측정했다. 파절면은 전계 방출 주사 전자 현미경(FE-SEM)으로 관찰했다. 매몰재와 접촉된 지르코니아 시편의 표면에 존재하는 원소의 변화를 X-선 스펙트럼(EDS, Bruker, Germany)로 분석했다. 3점 굴곡 강도 시험은 UN 군에서 가장 높은 값 (1265.5 MPa), F1 군에서 가장 낮은 값 (756.1 MPa)을 나타냈다. EDS 분석 결과 F1 그룹에서 가장 많은 Si가 검출되었으며 FE-SEM 분석 결과 가장 많은 계면 변화가 발생했다. 지르코니아는 매몰재로 매몰한 후 열가압성형을 실시할 경우 결합 계면 부근의 지르코니아의 상태변화로 인해 강도가 저하된다.
Tae-Yeon Kim; Seha Kim; Jeong-Hui Ji; Byoung Gu Lim; Sung Geun Oh; Jaewoo Shin; Junghwan Lee; Min-Ho Lee; Tae-Sung Bae. Effect of investment materials on 3-point flexural strength of heat pressed zirconia core. Korean Journal of Dental Materials 2020, 47, 211 -220.
AMA StyleTae-Yeon Kim, Seha Kim, Jeong-Hui Ji, Byoung Gu Lim, Sung Geun Oh, Jaewoo Shin, Junghwan Lee, Min-Ho Lee, Tae-Sung Bae. Effect of investment materials on 3-point flexural strength of heat pressed zirconia core. Korean Journal of Dental Materials. 2020; 47 (4):211-220.
Chicago/Turabian StyleTae-Yeon Kim; Seha Kim; Jeong-Hui Ji; Byoung Gu Lim; Sung Geun Oh; Jaewoo Shin; Junghwan Lee; Min-Ho Lee; Tae-Sung Bae. 2020. "Effect of investment materials on 3-point flexural strength of heat pressed zirconia core." Korean Journal of Dental Materials 47, no. 4: 211-220.
Cannulated screws, containing an internal hole for inserting a guide pin, are commonly used in the management of bone fractures. Cannulated Mg screws can be biodegraded easily because their increased surface area including that of the inner hole rapidly reacts with body fluids. To delay biodegradation of cannulated Mg screws and improve bone regeneration, we developed a specific type of screw by injecting it with gelatin hydrogels [10 wt % gelatin(gel) with 0.09 v/v % glutaraldehyde (cross-linker)] containing different concentrations (5, 10, or 25 μg/mL) of bone morphogenic proteins (BMPs). We analyzed the properties and biocompatibility of the screws with and without BMP-2 and found that the release rate of BMP-2 in the hydrogel changed proportionately with the degradation rate of the cross-linked hydrogel. Loading BMP-2 in the hydrogel resulted in sustained release of BMP-2 for 25 to 40 days or more. The degradation rate of BMP-2 hydrogels was inversely proportional to the concentration of BMP-2. The injection of the hydrogels in the cannulated screw delayed biodegradation inside of the screw by simulated body fluid. It also induced uniform corrosion and the precipitation of bioactive compounds onto the surface of the screw. In addition, osteoblast proliferation was very active near the BMP-2 hydrogels, depending on the BMP-2 concentration. The BMP-2 in the hydrogel improved cell differentiation. The cannulated screw injected with 10 μL/mL BMP-2 hydrogel prevented implant biodegradation and enhanced osteoconduction and osteointegration inside and outside the screw. In addition, the properties of BMP-2-loaded hydrogels can be changed by controlling the amount of the cross-linker and protein, which could be useful for tissue regeneration in other fields.
Seo-Young Kim; Yu-Kyoung Kim; Seong-Woo Chong; Kwang-Bok Lee; Min-Ho Lee. Osteogenic Effect of a Biodegradable BMP-2 Hydrogel Injected into a Cannulated Mg Screw. ACS Biomaterials Science & Engineering 2020, 6, 6173 -6185.
AMA StyleSeo-Young Kim, Yu-Kyoung Kim, Seong-Woo Chong, Kwang-Bok Lee, Min-Ho Lee. Osteogenic Effect of a Biodegradable BMP-2 Hydrogel Injected into a Cannulated Mg Screw. ACS Biomaterials Science & Engineering. 2020; 6 (11):6173-6185.
Chicago/Turabian StyleSeo-Young Kim; Yu-Kyoung Kim; Seong-Woo Chong; Kwang-Bok Lee; Min-Ho Lee. 2020. "Osteogenic Effect of a Biodegradable BMP-2 Hydrogel Injected into a Cannulated Mg Screw." ACS Biomaterials Science & Engineering 6, no. 11: 6173-6185.
본 연구의 목적은 리튬 디실리케이트 글라스 세라믹 단관의 제조 방법이 변연 및 내면 적합도에 미치는 영향을 평가하는 것이다. 리튬 디실리케이트 단관은 Hass 및 Ivoclar / Vivadent 사에서 제조된 열가압 성형용 잉곳과 CAD/CAM 밀링용 블록을 사용하여 제작하였다. 상악 중절치(#11)를 절단연 2 mm, 축면 1 mm로 절삭하고, 축벽 각도는 6°, 변연부는 deep chamfer 형태로 형성하였다. 제작방법에 따라 6개의 그룹으로 나누었다. ECM (IPS e.max CAD), EPC (Conventional wax-up & IPS e.max press), EPM (Wax block milling & IPS e.max Press), ABM (Amber Mill), APC (Conventional wax-up & Amber Press), APM (Wax block milling & Amber Press). 변연 및 내면 적합도의 측정 방법으로 실리콘 복제법을 이용하였고, 현미경을 사용하여 미리 정해놓은 14개의 기준점에서 측정하였다. 통계분석을 위해 Kruskal Wallis test를 시행하였고 사후검정으로 Mann Whitey test를 시행하였다. Bonferroni"s method로 제1종 오류를 보정하였다. 변연 부위에서는 ECM그룹과 EPM그룹 사이에서만 유의한 차이가 있었고 모든 그룹에서 임상적으로 허용가능한 범위(120 μm) 이내의 변연적합도를 나타냈다. Deep chamfer에서는 CAD/CAM 방식으로 제작한 ECM과 ABM그룹에서 적합도가 가장 우수하게 나타났다. 축벽과 절단연에서는 ECM그룹이 EPC와 EPM그룹에 비해 적합도가 우수하게 나타났고, ABM그룹과 APC그룹이 APM그룹에 비해 통계적으로 유의하게 우수한 적합도를 나타냈다.
Su-Yeon Choi; Seha Kim; Jeong-Hui Ji; Min-Ho Lee; Tae-Sung Bae. Effect of fabrication method of lithium disilicate crown on fitness. Korean Journal of Dental Materials 2020, 47, 157 -168.
AMA StyleSu-Yeon Choi, Seha Kim, Jeong-Hui Ji, Min-Ho Lee, Tae-Sung Bae. Effect of fabrication method of lithium disilicate crown on fitness. Korean Journal of Dental Materials. 2020; 47 (3):157-168.
Chicago/Turabian StyleSu-Yeon Choi; Seha Kim; Jeong-Hui Ji; Min-Ho Lee; Tae-Sung Bae. 2020. "Effect of fabrication method of lithium disilicate crown on fitness." Korean Journal of Dental Materials 47, no. 3: 157-168.
The formation of nanotube layer on titanium plate (N-Ti) facilitates an active reaction between bone cells and the material surface via efficient delivery of the surface materials of the dental implant into the tissues. Studies have reported that Korean Red ginseng extracts (KRGE) are involved in a variety of pharmacological activities: we investigated whether implantation with a KRGE-loaded N-Ti (GN-Ti) mini-implant affects osteogenesis and osseointegration. KRGE-loaded nanotubes were constructed by fabrication on pure Ti via anodization, and MC3T3-E1 cells were cultured on the N-Ti. N-Ti implants were subsequently placed on a rat’s edentulous mandibular site. New bone formation and bone mineral density were measured to analyze osteogenesis and osseointegration. KRGE-loaded N-Ti significantly increased the proliferation and differentiation of MC3T3-E1 cells compared with cells on pure-Ti without any KRGE loading. After 1-4 weeks, peri-implant tissue in the edentulous mandibular of the healed rat showed a remarkable increase in new bone formation and bone mineral density. In addition, high levels of the bone morphogenesis protein (BMP-2) and BMP-7, besides collagen, were expressed in the peri-implant tissues. Our findings suggest that KRGE-induced osteogenesis and osseointegration around the mini-implant may facilitate the clinical application of dental implants.
Myong-Hun Kang; Sook-Jeong Lee; Min-Ho Lee. Bone remodeling effects of Korean Red Ginseng extracts for dental implant applications. Journal of Ginseng Research 2020, 44, 823 -832.
AMA StyleMyong-Hun Kang, Sook-Jeong Lee, Min-Ho Lee. Bone remodeling effects of Korean Red Ginseng extracts for dental implant applications. Journal of Ginseng Research. 2020; 44 (6):823-832.
Chicago/Turabian StyleMyong-Hun Kang; Sook-Jeong Lee; Min-Ho Lee. 2020. "Bone remodeling effects of Korean Red Ginseng extracts for dental implant applications." Journal of Ginseng Research 44, no. 6: 823-832.
Initial corrosion resistance and bioactivity of biodegradable magnesium are major success factors for implant applications. Electrodeposition containing Ce prevent material damage and local corrosion resulting from implantation, and Ce penetrates hydrogels and consequently maximizes surface self-healing. Ce(NO3) was used to form an electrodeposition layer on magnesium, and hyaluronic acid(HA) was coated through hydrothermal treatment, which created a composite layer consisting of a polymer compound with Ce and a thick and stable MgO layer. The morphology, chemical structure, and scratch tests conducted on various surface treatments showed hydrothermally treated Ce to be the most effective self-healing and corrosion-resistance. In the electrodeposition of Ca/Ce, relatively reactive Ce quickly adhered to the surface, and according to the hydrothermal process, Ca and Ce ions diffused inward as water and HA penetrated the irregular layers. The cell viability of osteoblasts showed no toxicity with Ce, and the osteoblasts were showed the highest differentiation especially in treated with the HA hydrothermal group. Implantation into rat tibias resulted in stable bone marrow and osteoblast growth in the hydrothermal treatment group containing Ce. Consequently, Ce(OH)3/CeO2 penetrating the natural polymer HA had a self-healing ability and resistance to initial corrosion, local damage, and the biodegradation of the magnesium implant.
Yu-Kyoung Kim; Seo-Young Kim; Yong-Seok Jang; Il-Song Park; Min-Ho Lee. Bio-corrosion behaviors of hyaluronic acid and cerium multi-layer films on degradable implant. Applied Surface Science 2020, 515, 146070 .
AMA StyleYu-Kyoung Kim, Seo-Young Kim, Yong-Seok Jang, Il-Song Park, Min-Ho Lee. Bio-corrosion behaviors of hyaluronic acid and cerium multi-layer films on degradable implant. Applied Surface Science. 2020; 515 ():146070.
Chicago/Turabian StyleYu-Kyoung Kim; Seo-Young Kim; Yong-Seok Jang; Il-Song Park; Min-Ho Lee. 2020. "Bio-corrosion behaviors of hyaluronic acid and cerium multi-layer films on degradable implant." Applied Surface Science 515, no. : 146070.
Yu-Kyoung Kim; Yong-Seok Jang; Seo-Young Kim; Min-Ho Lee. Corrigendum to “Functions achieved by the hyaluronic acid derivatives coating and hydroxide film on bio-absorbed Mg” [Appl. Surf. Sci. 473 (2019) 31–39]. Applied Surface Science 2019, 512, 145087 .
AMA StyleYu-Kyoung Kim, Yong-Seok Jang, Seo-Young Kim, Min-Ho Lee. Corrigendum to “Functions achieved by the hyaluronic acid derivatives coating and hydroxide film on bio-absorbed Mg” [Appl. Surf. Sci. 473 (2019) 31–39]. Applied Surface Science. 2019; 512 ():145087.
Chicago/Turabian StyleYu-Kyoung Kim; Yong-Seok Jang; Seo-Young Kim; Min-Ho Lee. 2019. "Corrigendum to “Functions achieved by the hyaluronic acid derivatives coating and hydroxide film on bio-absorbed Mg” [Appl. Surf. Sci. 473 (2019) 31–39]." Applied Surface Science 512, no. : 145087.
Cannulated screws have a structure for inserting a guide wire inside them to effectively correct complicated fractures. Magnesium, an absorbable metal used to manufacture cannulated screws, may decompose in the body after a certain period of implantation. The hydrogel formed by hyaluronic acid (HA) and polygalacturonic acid (PGA) has been used into Mg-based cannulated screws to prevent bone resorption owing to the rapid corrosion of Mg with unfavorable mechanical properties and a high ambient pH. In addition, Ca ions were added to the gel for crosslinking the carboxyl groups to modify the gelation rate and physical properties of the gel. The developed hydrogels were injected into the Mg-based cannulated screws, after which they released HA and Ca. The possibility of the application of this system as a cannulated screw was evaluated based on the corrosion resistance, gel degradation rate, HA release, toxicity towards osteocytes, and experiments involving the implantation of the screws into the femurs of rats. Ca ions first bound to PGA and delayed the gelation time and dissolution rate. However, they interfered with HA binding and increased the elution of HA at the beginning of gel degradation. Ca(NO3)2 concentrations higher than 0.01M and low pH environments inhibited osteoblast differentiation and proliferation, owing to the elution of HA from the hydrogel. On the other hand, when the HA hydrogel with a proper amount of Ca was inserted into a magnesium screw, the degradation of Mg was delayed, and the presence of the gel contributed to new bone formation and osteocyte expansion.
Yu-Kyoung Kim; Seo-Young Kim; Se Hwan Lee; Min-Ho Lee; Kwang-Bok Lee. Stabilized Loading of Hyaluronic Acid-Containing Hydrogels into Magnesium-Based Cannulated Screws. ACS Biomaterials Science & Engineering 2019, 6, 715 -726.
AMA StyleYu-Kyoung Kim, Seo-Young Kim, Se Hwan Lee, Min-Ho Lee, Kwang-Bok Lee. Stabilized Loading of Hyaluronic Acid-Containing Hydrogels into Magnesium-Based Cannulated Screws. ACS Biomaterials Science & Engineering. 2019; 6 (1):715-726.
Chicago/Turabian StyleYu-Kyoung Kim; Seo-Young Kim; Se Hwan Lee; Min-Ho Lee; Kwang-Bok Lee. 2019. "Stabilized Loading of Hyaluronic Acid-Containing Hydrogels into Magnesium-Based Cannulated Screws." ACS Biomaterials Science & Engineering 6, no. 1: 715-726.
Guided bone regeneration using a perforated titanium membrane is actively used in oral and orthopedic surgery to provide a space for the subsequent filling of new bone in case of bone defect and to achieve proper bone augmentation and reconstruction. The surface modification of a titanium membrane using a strontium-substituted calcium phosphate coating has become a popular trend to provide better bioactivity and biocompatibility on membrane for improving the bone regeneration because strontium can stimulate not only the differentiation of osteoblasts but also inhibits the differentiation of osteoclasts. The strontium-doped calcium phosphate coating on the titanium mesh was formed by the cyclic precalcification method, and its effects on bone regeneration were evaluated by in vitro analysis of osteogenesis-related gene expression and in vivo evaluation of osteogenesis of titanium mesh using rat calvarial defect model in this study. It was identified that the strontium-doped calcium phosphate treated mesh showed higher expression of all genes related to osteogenesis in the osteoblast cells, and resulted in new bone formation with better osseointegration with mesh in rat calvarial defect, in comparison with the results of untreated and calcium phosphate treated meshes.
Thuy-Duong Thi Nguyen; Yong-Seok Jang; Yu-Kyoung Kim; Seo-Young Kim; Min Ho Lee; Tae-Sung Bae. Osteogenesis-Related Gene Expression and Guided Bone Regeneration of a Strontium-Doped Calcium–Phosphate-Coated Titanium Mesh. ACS Biomaterials Science & Engineering 2019, 5, 6715 -6724.
AMA StyleThuy-Duong Thi Nguyen, Yong-Seok Jang, Yu-Kyoung Kim, Seo-Young Kim, Min Ho Lee, Tae-Sung Bae. Osteogenesis-Related Gene Expression and Guided Bone Regeneration of a Strontium-Doped Calcium–Phosphate-Coated Titanium Mesh. ACS Biomaterials Science & Engineering. 2019; 5 (12):6715-6724.
Chicago/Turabian StyleThuy-Duong Thi Nguyen; Yong-Seok Jang; Yu-Kyoung Kim; Seo-Young Kim; Min Ho Lee; Tae-Sung Bae. 2019. "Osteogenesis-Related Gene Expression and Guided Bone Regeneration of a Strontium-Doped Calcium–Phosphate-Coated Titanium Mesh." ACS Biomaterials Science & Engineering 5, no. 12: 6715-6724.
This study was conducted to test the hypothesis that heat-bonding with a liner positively affects the bond strength and fracture resistance of an implant-supported glass–ceramic crown bonded to a zirconia abutment produced by a computer-aided design/computer-aided milling (CAD/CAM) procedure. Lithium disilicate-reinforced Amber Mill-Q glass ceramic blocks were bonded to 3 mol% yttria stabilized tetragonal zirconia polycrystal (3Y-TZP) blocks by heat-bonding with a liner or cementation with a dual-cure self-adhesive resin cement for a microtensile bond strength test. CAD/CAM implant-supported glass ceramic crowns were produced using Amber Mill-Q blocks and bonded to a milled 3Y-TZP zirconia abutments by heat-bonding or cementation for a fracture test. A statistical analysis was conducted to investigate the significant differences between the experimental results. The mode of failure was analyzed using high-resolution field emission scanning electron microscopy. Chemical bonding was identified at the interface between the zirconia ceramic and liner. The mean tensile bond strength of the liner-bonded group was significantly higher than that of the cement-bonded group. The initial chipping strength of the liner-bonded group was significantly higher than that of the cement-bonded group, although no statistically significant difference was found for the fracture strength. The mode of failure was mixed with cohesive fracture through the liner, whereas the cement-bonded group demonstrated adhesive failure at the interface of bonding.
Yong-Seok Jang; Sang-Hoon Oh; Won-Suck Oh; Min-Ho Lee; Jung-Jin Lee; Tae-Sung Bae. Effects of Liner-Bonding of Implant-Supported Glass–Ceramic Crown to Zirconia Abutment on Bond Strength and Fracture Resistance. Materials 2019, 12, 2798 .
AMA StyleYong-Seok Jang, Sang-Hoon Oh, Won-Suck Oh, Min-Ho Lee, Jung-Jin Lee, Tae-Sung Bae. Effects of Liner-Bonding of Implant-Supported Glass–Ceramic Crown to Zirconia Abutment on Bond Strength and Fracture Resistance. Materials. 2019; 12 (17):2798.
Chicago/Turabian StyleYong-Seok Jang; Sang-Hoon Oh; Won-Suck Oh; Min-Ho Lee; Jung-Jin Lee; Tae-Sung Bae. 2019. "Effects of Liner-Bonding of Implant-Supported Glass–Ceramic Crown to Zirconia Abutment on Bond Strength and Fracture Resistance." Materials 12, no. 17: 2798.
Guided bone regeneration is a therapeutic method that uses a barrier membrane to provide space available for new bone formation at sites with insufficient bone volume. Magnesium with excellent biocompatibility and mechanical properties has been considered as a promising biodegradable material for guided bone regeneration; however, the rapid degradation rate in the physiological environment is a problem to be solved. In this study, surface modification of pure magnesium mesh was conducted by plasma electrolytic oxidation and hydrothermal treatment to form a densely protective layer on the Mg substrate. The protective layer mainly consisted of Mg(OH)2 with the amorphous calcium phosphate. Then, weight loss measurement and Micro-CT imaging were performed after an immersion test in a simulated body fluid. The effect of surface modification of the magnesium mesh on the guided bone regeneration was evaluated through an in vivo test using the rat calvarial defect model. The biodegradation of the magnesium mesh was identified to be significantly retarded. Additionally, the surface modification of Mg also can improve the bone volume and bone density of calvarial defect in comparison with that of the pristine Mg mesh.
Shuang Wu; Yong-Seok Jang; Yu-Kyoung Kim; Seo-Young Kim; Seung-O Ko; Min-Ho Lee. Surface Modification of Pure Magnesium Mesh for Guided Bone Regeneration: In Vivo Evaluation of Rat Calvarial Defect. Materials 2019, 12, 2684 .
AMA StyleShuang Wu, Yong-Seok Jang, Yu-Kyoung Kim, Seo-Young Kim, Seung-O Ko, Min-Ho Lee. Surface Modification of Pure Magnesium Mesh for Guided Bone Regeneration: In Vivo Evaluation of Rat Calvarial Defect. Materials. 2019; 12 (17):2684.
Chicago/Turabian StyleShuang Wu; Yong-Seok Jang; Yu-Kyoung Kim; Seo-Young Kim; Seung-O Ko; Min-Ho Lee. 2019. "Surface Modification of Pure Magnesium Mesh for Guided Bone Regeneration: In Vivo Evaluation of Rat Calvarial Defect." Materials 12, no. 17: 2684.
This study compares the mechanical properties and wear ability of five CAD/CAM (computer-aided design/computer-aided manufacturing) millable dental blocks. All the discs, including Amber Mill Hybrid, Vita Enamic, Katana Avencia, Lava Ultimate, and Amber Mill, were cut in dimensions of 1.2 mm in thickness and 12 mm in diameter, polished to a machined surface, and immersed in distilled water for seven days. Vickers hardness was measured and the indentations were observed using microscope. The discs were brushed under a 150 g load. Mean surface roughness (Ra) and topography were determined after 100,000 cycles. Finally the biaxial flexure strength of the discs was measured and the broken surfaces were observed using scanning electron microscopy (SEM). The data was subjected to Weibull analysis. All data were analyzed by one-way analysis (ANOVA). The results of Vickers hardness are shown as: Amber Mill > Vita Enamic > Amber Mill Hybrid > Lava Ultimate > Katana Avencia. Katana Avencia showed the highest volume percentage reduction and the roughest surface after toothbrushing. The biaxial flexural strength is shown as: Amber Mill > Katana Avencia > Lava Ultimate > Amber Mill Hybrid > Vita Enamic. All the tested materials exhibited varying degrees of mass loss and surface roughness. The properties of the composite materials are related to the filler content, filler volume, and polymerization methods.
Ruizhi Yin; Yong-Seok Jang; Min-Ho Lee; Tae-Sung Bae; Yin; Jang; Lee; Bae. Comparative Evaluation of Mechanical Properties and Wear Ability of Five CAD/CAM Dental Blocks. Materials 2019, 12, 2252 .
AMA StyleRuizhi Yin, Yong-Seok Jang, Min-Ho Lee, Tae-Sung Bae, Yin, Jang, Lee, Bae. Comparative Evaluation of Mechanical Properties and Wear Ability of Five CAD/CAM Dental Blocks. Materials. 2019; 12 (14):2252.
Chicago/Turabian StyleRuizhi Yin; Yong-Seok Jang; Min-Ho Lee; Tae-Sung Bae; Yin; Jang; Lee; Bae. 2019. "Comparative Evaluation of Mechanical Properties and Wear Ability of Five CAD/CAM Dental Blocks." Materials 12, no. 14: 2252.
Background: Biofilm formation and microbial colonization on the surface of implant devices may cause dental caries and peri-implantitis. Therefore, various surface treatments have been developed to improve the antibacterial activity of titanium implant. Methods: Silver-loaded polydopamine coating was formed by immersing pure titanium in dopamine hydrochloride/HCl buffer solution for 24 h in 50 mL silver nitrate solutions with different concentrations for 30 min. Microbial growth inhibition and microbial growth curve analyses for bacterial solutions of Streptococcus mutans and Porphyromonas gingivalis incubated with the specimens were respectively conducted by counting the numbers of colonies on agar solid medium and by measuring absorbance using enzyme-linked immunosorbent assay reader. Results: Silver nanoparticles were uniformly distributed over the whole surface of the polydopamine and silver-coated titanium specimens. The numbers of microbial colonies for both bacteria cultured with surface-modified titanium were significantly lower than those cultured with uncoated titanium. When Streptococcus mutans and Porphyromonas gingivalis were cultured with surface-modified titanium, the lag phase of the growth curves for both bacteria was continually maintained, whereas the lag phase for Streptococcus mutans and Porphyromonas gingivalis changed to exponential phase after 9 and 15 h, respectively, when both bacteria were cultured with uncoated titanium. Conclusion: It was confirmed that the coating of polydopamine and silver on the surface of titanium effectively retards the microbial growth, which can cause the formation of biofilm and pathogenesis of gum disease in the mouth.
Soo-Hyoen Choi; Yong-Seok Jang; Jong-Hwa Jang; Tae-Sung Bae; Sook-Jeong Lee; Min-Ho Lee. Enhanced antibacterial activity of titanium by surface modification with polydopamine and silver for dental implant application. Journal of Applied Biomaterials & Functional Materials 2019, 17, 1 .
AMA StyleSoo-Hyoen Choi, Yong-Seok Jang, Jong-Hwa Jang, Tae-Sung Bae, Sook-Jeong Lee, Min-Ho Lee. Enhanced antibacterial activity of titanium by surface modification with polydopamine and silver for dental implant application. Journal of Applied Biomaterials & Functional Materials. 2019; 17 (3):1.
Chicago/Turabian StyleSoo-Hyoen Choi; Yong-Seok Jang; Jong-Hwa Jang; Tae-Sung Bae; Sook-Jeong Lee; Min-Ho Lee. 2019. "Enhanced antibacterial activity of titanium by surface modification with polydopamine and silver for dental implant application." Journal of Applied Biomaterials & Functional Materials 17, no. 3: 1.
Hydrogen gas formed by magnesium (Mg) screw corrosion can accumulate around the implant and create bone cysts, long-term osteolysis lesions, and bone healing delay. Thus, several authors currently do not recommend Mg implants for clinical use. In contrast, bone morphogenetic proteins (BMP)-2 have a very strong osteoinductive activity. The purpose of this study was to evaluate the effect of rhBMP-2/hydroxyapatite (HA) inside specially designed Mg cannulated screws in a rabbit femur model for hydrogen gas formation avoidance. Fifteen rabbits underwent randomly different cannulated Mg screw implantation in both distal femora; 30 femora were divided into three groups depending on the materials fill in the cannulated Mg screw: control group (Mg screw with no treatment), HA group (Mg screw with HA), and BMP-2/HA group (Mg screw with a composite BMP-2/HA). Plain radiography, micro-CT, and histological analysis were accomplished, and the ability to release BMP-2 of the screws was evaluated by immersion of both the screw with no treatment and screw with a composite BMP-2/HA into the SBF for up to 7 days. X-ray assessment found the gas shadow around the implant was slightly smaller in the BMP-2/HA group than the HA and control groups at 8 weeks. Micro-CT analysis demonstrated statistically significant higher new bone formation in the BMP-2/HA group than the other groups, respectively, which also correlated with a decreased gas volume. Histological analysis showed higher osteointegration between implants and host femurs in the BMP-2/HA group than the HA and control groups at 12 weeks. This study indicates that the combination of BMP-2/HA within Mg screws enhances new bone formation and therefore has the potential to decrease the complications of hydrogen gas formation around these implants.
Le Hoang Nam Dang; Yu Kyoung Kim; Seo Young Kim; Kuk Jin Lim; Ken Bode; Min Ho Lee; Kwang Bok Lee. Radiographic and histologic effects of bone morphogenetic protein-2/hydroxyapatite within bioabsorbable magnesium screws in a rabbit model. Journal of Orthopaedic Surgery and Research 2019, 14, 117 .
AMA StyleLe Hoang Nam Dang, Yu Kyoung Kim, Seo Young Kim, Kuk Jin Lim, Ken Bode, Min Ho Lee, Kwang Bok Lee. Radiographic and histologic effects of bone morphogenetic protein-2/hydroxyapatite within bioabsorbable magnesium screws in a rabbit model. Journal of Orthopaedic Surgery and Research. 2019; 14 (1):117.
Chicago/Turabian StyleLe Hoang Nam Dang; Yu Kyoung Kim; Seo Young Kim; Kuk Jin Lim; Ken Bode; Min Ho Lee; Kwang Bok Lee. 2019. "Radiographic and histologic effects of bone morphogenetic protein-2/hydroxyapatite within bioabsorbable magnesium screws in a rabbit model." Journal of Orthopaedic Surgery and Research 14, no. 1: 117.
Yong-Seok Jang; Jae-Yoen Kang; Kang-Gyu Lee; Chung-Ha Lim; Min-Ho Lee; Tae-Sung Bae. Evaluation of bioactivity and osseointegration for ti-6al-4v alloy implant modified by anodic oxidation and cyclic precalcification treatments. Korean Journal of Dental Materials 2019, 46, 43 -52.
AMA StyleYong-Seok Jang, Jae-Yoen Kang, Kang-Gyu Lee, Chung-Ha Lim, Min-Ho Lee, Tae-Sung Bae. Evaluation of bioactivity and osseointegration for ti-6al-4v alloy implant modified by anodic oxidation and cyclic precalcification treatments. Korean Journal of Dental Materials. 2019; 46 (1):43-52.
Chicago/Turabian StyleYong-Seok Jang; Jae-Yoen Kang; Kang-Gyu Lee; Chung-Ha Lim; Min-Ho Lee; Tae-Sung Bae. 2019. "Evaluation of bioactivity and osseointegration for ti-6al-4v alloy implant modified by anodic oxidation and cyclic precalcification treatments." Korean Journal of Dental Materials 46, no. 1: 43-52.
Multi walled carbon nanotubes-hydroxyapatite (MWCNTs-HA) with various contents of MWCNTs was synthesized using the sol-gel method. MWCNTs-HA composites were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). HA particles were generated on the surface of MWCNT. Produced MWCNTs-HA nanocomposites were coated on pure titanium (PT). Characteristic of the titanium coated MWCNTs-HA was evaluated by field-emission scanning electron microscopy (FE-SEM) and XRD. The results show that the titanium surface was covered with MWCNTs-HA nanoparticles and MWCNTs help form the crystalized hydroxyapatite. Furthermore, the MWCNTs-HA coated titanium was investigated for in vitro cellular responses. Cell proliferation and differentiation were improved on the surface of MWCNT-HA coated titanium.
Jung-Eun Park; Yong-Seok Jang; Tae-Sung Bae; Min-Ho Lee. Biocompatibility Characteristics of Titanium Coated with Multi Walled Carbon Nanotubes—Hydroxyapatite Nanocomposites. Materials 2019, 12, 224 .
AMA StyleJung-Eun Park, Yong-Seok Jang, Tae-Sung Bae, Min-Ho Lee. Biocompatibility Characteristics of Titanium Coated with Multi Walled Carbon Nanotubes—Hydroxyapatite Nanocomposites. Materials. 2019; 12 (2):224.
Chicago/Turabian StyleJung-Eun Park; Yong-Seok Jang; Tae-Sung Bae; Min-Ho Lee. 2019. "Biocompatibility Characteristics of Titanium Coated with Multi Walled Carbon Nanotubes—Hydroxyapatite Nanocomposites." Materials 12, no. 2: 224.