Cytochrome P450 (CYP450) is a group of enzymes that play an essential role in Phase I metabolism, with 57 functional genes classified into 18 families in the human genome, of which the CYP1, CYP2, and CYP3 families are prominent. Beyond drug metabolism, CYP enzymes metabolize endogenous compounds such as lipids, proteins, and hormones to maintain physiological homeostasis. Thus, dysregulation of CYP450 enzymes can lead to different endocrine disorders. Moreover, CYP450 enzymes significantly contribute to fatty acid metabolism, cholesterol synthesis, and bile acid biosynthesis, impacting cellular physiology and disease pathogenesis. Their diverse functions emphasize their therapeutic potential in managing hypercholesterolemia and neurodegenerative diseases. Additionally, CYP450 enzymes are implicated in the onset and development of illnesses such as cancer, influencing chemotherapy outcomes. Assessment of CYP450 enzyme expression and activity aids in evaluating liver health state and differentiating between liver diseases, guiding therapeutic decisions, and optimizing drug efficacy. Understanding the roles of CYP450 enzymes and the clinical effect of their genetic polymorphisms is crucial for developing personalized therapeutic strategies and enhancing drug responses in diverse patient populations.

Heavy metals like lead, mercury, cadmium, and arsenic are environmental pollutants that accumulate in ecosystems and pose significant health risks to humans and wildlife, primarily through food chain contamination where plants absorb heavy metals, affecting their growth and threatening consumer health. Cognitive and cardiovascular functions are particularly affected by exposure to heavy metals even at low concentrations through the induction of oxidative stress. Various analytical techniques are used in measuring heavy metals in different environmental and biological samples. The atomic absorption spectroscopy (AAS) offers low cost, simplicity, and portability but lacks sensitivity for certain metals. Although more sensitive, the high cost of inductively coupled plasma mass spectrometry (ICP-MS) may limit laboratory accessibility. The inductively coupled plasma with atomic emission spectrometry (ICP-AES) is known for its broad dynamic linear range and ability to identify minute variations in concentration. Atomic fluorescence spectrometry (AFS) is considered a powerful tool for quantifying heavy metals due to its high sensitivity, low detection limits, and wide linear range. The current article reviews heavy metal pollution's impact on health and spectrometric techniques for the detection of these contaminants. This may help efforts of international, and regional policies towards preventing this health hazard problem.