The aim of the current study was to investigate the relationship between the prevailing and desirable organizational cultures and the job satisfaction of employees in primary health care structures as a development strategy. To evaluate organizational culture we used the Culture Assessment Instrument (OCAI) questionnaire, whose authors are Cameron and Quinn (1999). The measurement of job satisfaction was carried out with Spector’s Job Satisfaction Survey (JSP) (1985). The tool used to analyze results was the statistical analysis program SPSS V.22.0. Internal affinity audits, regularity checks, non-parametric controls, ANOVA and Kruskal–Wallis variance controls were performed. The significance level was set at 0.05 for all statistical analyses. This study showed that bureaucracy was predominant, and for the future, there is a desire for change in the direction of human resources. Job satisfaction was at a moderate level, while there was a positive average for working conditions and a negative average for promotion opportunities.