Boletes are one of the most common groups of fungi in temperate, subtropical, and tropical ecosystems. In Mexico, the northern region has mainly been explored in terms of bolete diversity. This study describes a new genus and seven new species based on macromorphological, micromorphological, molecular, phylogenetic, and ecological data. Garcileccinum gen. nov. is typified with G. salmonicolor based on multigene phylogenetic analysis of nrLSU, RPB2, and TEF1, and it is closely related to Leccinum and Leccinellum. Garcileccinum viscosum and G. violaceotinctum are new combinations. Boletellus minimatenebris (ITS, nrLSU, and RPB2), Cacaoporus mexicanus (RPB2 and ATP6), Leccinum oaxacanum, Leccinum juarenzense (nrLSU, RPB2, and TEF1), Tylopilus pseudoleucomycelinus (nrLSU and RPB2), and Xerocomus hygrophanus (ITS, nrLSU, and RPB2) are described as new species. Boletus neoregius is reclassified as Pulchroboletus neoregius comb. nov. based on morphological and multigene phylogenetic analysis (ITS and nrLSU), and its geographic distribution is extended to Central Mexico, since the species was only known from Costa Rica. Furthermore, T. leucomycelinus is a new record from Mexico. This study contributes to increasing our knowledge of boletes and expands the diversity found in Mexican forests.

The genus Tarzetta is distributed mainly in temperate forests and establishes ectomycorrhizal associations with angiosperms and gymnosperms. Studies on this genus are scarce in México. A visual, morphological, and molecular (ITS-LSU) description of T. americupularis, T. cupressicola, T. davidii, T. durangensis, T. mesophila, T. mexicana, T. miquihuanensis, T. poblana, T. pseudobronca, T. texcocana, and T. victoriana was carried out in this work, associated with Abies, Quercus, and Pinus. The results of SEM showed an ornamented ascospores formation by Mexican Taxa; furthermore, the results showed that T. catinus and T. cupularis are only distributed in Europe and are not associated with any American host.

Hydnobolites oaxacanus is described using molecular and morphological data. This new species is characterized by the whitish ascomata with orange spots, grey gleba, and 4-spored asci with ascospores of 17–45 × 15–20 µm. The new species differs from other American Hydnobolites such as H. californicum and H. cerebriformis by the number of ascospores per asci, and larger ascospore size and ornamentation. It is putatively associated with Quercus castanea in the Quercus-Pinus dry mixed forest. Photographs, descriptions, and molecular phylogeny of the ITS region are presented.

Neottiella albocincta (Berk. & M.A. Curtis) Sacc., N. rutilans (Fr.) Dennis, Octospora maireana (Seaver) Yei Z. Wang, and O. texensis Benkert are reported for the first time from Mexico. These species, all belonging to the family Pyronemataceae, have close relationships with several species of mosses such as those in the genera Archidium Brid., Atrichum P. Beauv., and Polytrichum Hedw. The specimens were collected in montane cloud forest, riparian and Quercus forest in the states of Puebla and Tamaulipas. With the addition of these species, the number of bryosymbiotic fungi in Mexico increases to five.

Volvariella cubensis (Murrill) Shaffer is distinguished by the dark-gray and fibrillose pileus, pinkish free gills and the thick, brownish volva. This species is described and illustrated from the state of Tamaulipas in northeastern Mexico and constitutes the first record for the country, as well as the northernmost occurrence of V. cubensis in America. Descriptions, photographs, and discussion are presented.