Plants produce and accumulate stress-resistant substances when exposed to abiotic stress, which involves a protein conversion mechanism that breaks down stress-damaged proteins and supplies usable amino acids. Eukaryotic protein turnover is mostly driven by the ubiquitination pathway. Among the three enzymes required for protein degradation, E3 ubiquitin ligase plays a pivotal role in most cells, as it determines the specificity of ubiquitination and selects target proteins for degradation. In this study, to investigate the function of OsPUB7 (Plant U-box gene in Oryza sativa), we constructed a CRISPR/Cas9 vector, generated OsPUB7 gene-edited individuals, and evaluated resistance to abiotic stress using gene-edited lines. A stress-tolerant phenotype was observed as a result of drought and salinity stress treatment in the T₂ OsPUB7 gene-edited null lines (PUB7-GE) lacking the T-DNA. In addition, although PUB7-GE did not show any significant change in mRNA expression analysis, it showed lower ion leakage and higher proline content than the wild type (WT). Protein–protein interaction analysis revealed that the expression of the genes (OsPUB23, OsPUB24, OsPUB66, and OsPUB67) known to be involved in stress increased in PUB7-GE and this, by forming a 1-node network with OsPUB66 and OsPUB7, acted as a negative regulator of drought and salinity stress. This result provides evidence that OsPUB7 will be a useful target for both breeding and future research on drought tolerance/abiotic stress in rice.

We reconstruct the processes of sedimentation at the Suyanggae Paleolithic sites, geomorphologically characterized by fl uvial terraces in the Namhan River, the base level of which is higher than the present river bottom. The fl uvial sedimentary deposits, slope deposits, and paleosols are the main units of surfi cial deposits, constituting the site materials of Suyanggae Loc. VI. According to the representative profi les of the site, the deposits comprise sands and gravel at the bottom part, while sands and fl ooding muds with occasional intercalations of reddish-brown slope muds, as well as rounded or subangular cobbles or boulders, dominate the middle to upper part. Regarding the terrace’s morpho-stratigraphy, Suyanggae Loc. VI is located above the low (second) fl uvial terrace. Considering the chronology of site material formations, Suyanggae Loc. VI was formed in the last glacial period. On the basis of radiocarbon dates obtained for the charcoals from Suyanggae Loc. VI, the age of cultural layers is determined. Cultural layer 2 was formed in the late Upper Paleolithic, and CL 3 and CL 4 are associated with the early Upper Paleolithic. The archaeological assemblage of Suyanggae Loc. VI is described: lithic artifacts of CL 2 are characterized by abundant microblades (ca 20 ka BP, Last Glacial Maximum), while those of CL 3 and CL 4 are associated with tanged points and blades (36–42 ka BP, middle of the last glacial period). Especially noted are a line-engraved cobble stone excavated from the sedimentary matrix of fl uvial origin, and a face-engraved pebble stone found in fl ooding muds. The fi nds are interpreted as manifestation of symbolic human behavior.

Archaeological excavations led by Yung-jo Lee and Jong-yoon Woo were carried out twice at the Sorori paleolithic site, Cheongju, in the Republic of Korea, at the upper stream of the Geumgang river, the Miho riverside. A total of 127 rice seeds were excavated, including 18 ancient rice and 109 Quasi-rice, in 1998 and 2001. At the first excavation, eleven short japonica-type ancient rice and one slender smooth ancient rice with two kinds of Quasi-rice were excavated. The average length of the 11 short rice grains obtained from the first and second excavation was 7.19 mm and the average width was 3.08 mm, respectively. The Quasi-rice are apparently different from the rice and do not have bi-peak protuberances on their glume surface. At the second excavation, six short ancient rice chaffs and some Quasi-rice 2 were found. These short-grained ancient rice were comparable to the ancient rice that were excavated at the Illsan Neolithic site. Geologists and radiologists confirmed that the peat layer in which the rice found was older than 15,000 years. In this study, the morphological characteristics, crushing, and DNA band patterns related to the genetic polymorphism of rice grains in Cheongju Sorori were compared and analyzed for genetic similarities and differences with wild rice, weed rice, and modern rice. The morphological, ecological, and physiological variations in rice grains excavated from the Sorori site were presumed to denote the origin of rice domestication in Korea. It is also suggested that the results of the DNA sequencing of excavated rice are very important clues in estimating the origin of the early domestication of rice.