Growing wastewater treatment demands drive reliance on polymeric materials to facilitate the process, and traditional fossil-based polymers face limitations, prompting the exploration of sustainable alternatives. Biopolymers derived from renewable sources (chitosan, keratin, and cellulose) offer advantages like biodegradability and enhanced adsorption. This review provides an overview of the potential biopolymer-based composites for removing various pollutants (pesticides, heavy metals, and dyes) and highlights the importance of reusing spent adsorbents for multiple applications.

The prevalence and adverse impacts of microplastics requires the identification of science-based abatement measures. Electrocoagulation treatment is a cost-effective oxidation process that removes numerous pollutants, including to some extent, microplastics. The performance of a custom-built electrocoagulation reactor was determined by calculating the removal efficiency. The effects of the oxidation process on polymer types (polyamide (PA), polyethylene (PE), polyethylene terephthalate (PET) and polypropylene (PP)) and shapes (fibres and fragments) were investigated in synthetic wastewater and laundry wastewater. The calculated removal efficiency suggested that electrocoagulation treatment was an effective technology for microplastics abatement. More fibres tended to be removed than fragments, viz. 92% fibres removed versus 88% fragments. The findings also demonstrated that specific polymers were preferentially removed, viz. PET > LDPE > PP > PA. Further analysis indicated that the electrocoagulation treatment affected microplastic polymers physically, viz. flaking and changed surface conditions, as well as chemically, viz. changes in vibrational energies of C-O-C stretching bonds, C=O stretching bonds, C-H stretching bonds and formation of reactive oxygen species (ROS). Our findings indicate that whilst seemingly effective, electrocoagulation treatment induces changes to microplastic polymers that could beneficially lead to degradation, and/or further fragmentation or breakdown and thereby potentially generating more bioavailable toxic nanoplastic byproducts.

Pyroligneous acid (PA) is often used in agriculture as a plant growth and yield enhancer. However, the influence of PA application on soil microorganisms is not often studied. Therefore, in this study, we investigated the effect of PA (0.01–5% w/w in soil) on the microbial diversity in two different soils. At the end of eight weeks of incubation, soil microbial community dynamics were determined by Illumina-MiSeq sequencing of 16S rRNA gene amplicons. The microbial composition differed between the lower (0.01% and 0.1%) and the higher (1% and 5%) concentration in both PA spiked soils. The lower concentration of PA resulted in higher microbial diversity and dehydrogenase activity (DHA) compared to the un-spiked control and the soil spiked with high PA concentrations. Interestingly, PA-induced plant growth-promoting bacterial (PGPB) genera include Bradyrhizobium, Azospirillum, Pseudomonas, Mesorhizobium, Rhizobium, Herbaspiriluum, Acetobacter, Beijerinckia, and Nitrosomonas at lower concentrations. Additionally, the PICRUSt functional analysis revealed the predominance of metabolism as the functional module’s primary component in both soils spiked with 0.01% and 0.1% PA. Overall, the results elucidated that PA application in soil at lower concentrations promoted soil DHA and microbial enrichment, particularly the PGPB genera, and thus have great implications for improving soil health.

Bioaugmentation effectively enhances microbial bioremediation of hazardous polycyclic aromatic hydrocarbons (PAHs) from contaminated environments. While screening for pyrene-degrading bacteria from a former manufactured gas plant soil (MGPS), the mixed enrichment culture was found to be more efficient in PAHs biodegradation than the culturable pure strains. Interestingly, analysis of 16S rRNA sequences revealed that the culture was dominated by a previously uncultured member of the family Rhizobiaceae. The culture utilized C1 and other methylotrophic substrates, including dimethylformamide (DMF), which was used as a solvent for supplementing the culture medium with PAHs. In the liquid medium, the culture rapidly degraded phenanthrene, pyrene, and the carcinogenic benzo(a)pyrene (BaP), when provided as the sole carbon source or with DMF as a co-substrate. The efficiency of the culture in the bioremediation of PAHs from the MGPS and a laboratory waste soil (LWS) was evaluated in bench-scale slurry systems. After 28 days, 80% of Σ16 PAHs were efficiently removed from the inoculated MGPS. Notably, the bioaugmentation achieved 90% removal of four-ringed and 60% of highly recalcitrant five- and six-ringed PAHs from the MGPS. Likewise, almost all phenanthrene, pyrene, and 65% BaP were removed from the bioaugmented LWS. This study highlights the application of the methylotrophic enrichment culture dominated by an uncultured bacterium for the efficient bioremediation of PAHs.

In this study, we report the first draft genome sequence of Microbacterium esteraromaticum MM1, isolated from golf course soil in South Australia. The genome possesses genes for the hydrolysis of organophosphorus (OP) pesticides and polycyclic aromatic hydrocarbon (PAH) degradation.