In the past decade, next-generation sequencing (NGS) enabled the generation of genomic data in a cost-effective, high-throughput manner. The most recent third-generation sequencing technologies produce longer reads; however, their error rates are much higher, which complicates the assembly process. This generates time- and space- demanding long-read assemblers. Moreover, the advances in these technologies have allowed portable and real-time DNA sequencing, enabling in-field analysis. In these scenarios, it becomes crucial to have more efficient solutions that can be executed in computers or mobile devices with minimum hardware requirements. We re-implemented an existing assembler devoted for long reads, more concretely Flye, using compressed data structures. We then compare our version with the original software using real datasets, and evaluate their performance in terms of memory requirements, execution speed, and energy consumption. The assembly results are not affected, as the core of the algorithm is maintained, but the usage of advanced compact data structures leads to improvements in memory consumption that range from 22% to 47% less space, and in the processing time, which range from being on a par up to decreases of 25%. These improvements also cause reductions in energy consumption of around 3-8%, with some datasets obtaining decreases up to 26%.

The quantification of the SARS-CoV-2 RNA load in wastewater has emerged as a useful tool to monitor COVID–19 outbreaks in the community. This approach was implemented in the metropolitan area of A Coruña (NW Spain), where wastewater from a treatment plant was analyzed to track the epidemic dynamics in a population of 369,098 inhabitants. Viral load detected in the wastewater and the epidemiological data from A Coruña health system served as main sources for statistical models developing. Regression models described here allowed us to estimate the number of infected people (R² = 0.9), including symptomatic and asymptomatic individuals. These models have helped to understand the real magnitude of the epidemic in a population at any given time and have been used as an effective early warning tool for predicting outbreaks in A Coruña municipality. The methodology of the present work could be used to develop a similar wastewater-based epidemiological model to track the evolution of the COVID–19 epidemic anywhere in the world where centralized water-based sanitation systems exist.