Microplastics and nanoplastics are hazardous to ecosystems, wildlife, and through seafood, also for human health. Due to biological, chemical, and physical characteristics, nanoplastics can slip through cell membranes, being even more toxicologically important than microplastics. Thermal Desorption - Proton Transfer Reaction - Mass Spectrometry was used to analyze mussels from the Apulian region, Italy. All the analyzed organisms have plastics (values ranging from 10 to 187 ng of microplastics and nanoplastics per dry weight mg). The highest mass of plastics was detected in the size groups >2.2 µm (218 ng per dry weight mg) and 20–200 nm (187 ng per dry weight mg). Upscaling data we estimated that people in Europe could ingest more than 2 mg of nanoplastics per year through seafood consumption. The detected presence of nanoplastics in farmed mussels here presented contributes to establishing a baseline for monitoring these pollutants.

Raman spectroscopy was applied to quantitatively and qualitatively characterize microplastics (MPs) in bottled water packaged in three different materials: polyethylene terephthalate (PET), recycled PET (rPET) and glass. The results showed a low mean concentration of MPs in all samples, with higher values in glass bottles (8.65 ± 5.39 p L⁻¹) than in PET (5.09 ± 3.28 p L⁻¹) or rPET (3.33 ± 1.34 p L⁻¹) bottles. Through the use of a method capable of detecting smaller particles, MPs of 20–100 μm were dominant and fragments were the most abundant particle shape. PET was the prevalent polymer in PET bottles underlying the possible contribution of packaging in MP contamination, while polyethylene (PE) with additives prevailed in rPET and glass bottles, suggesting a contribution from the cap. A standardized protocol would allow comparable data to be obtained and allow an objective assessment of exposure, in view of plans to monitor contaminants of emerging concern (including MPs) under recent European legislation.

Plastic pollution is a worldwide problem especially in the marine environment. Plastic items once fragmented into microplastics (MPs), can be captured by different marine species. Benthic filter feeders like sponges and polychaetas, due to their trophic strategy, are highly exposed to MPs pollution. Herein a simple but effective method to digest the fan worm Sabella spallanzanii and the calcareous sponge Paraleucilla magna is presented: a solution with KOH and H₂O₂ was able to remove quantitatively (more than 98 %) the organic matter in 3 h while an acid treatment dissolved most of spicules and chaetes in less than 30 min. MPs were easily identified both microscopically and spectroscopically on filters. Quantification in animals collected from the same environment showed that, on average, sponges accumulate fewer MPs than polychaetes (66 ± 31 and 117 ± 46 particles/g dry weight, respectively). The plastic recovery of the method was validated using three different approaches (spiking of standard PS microspheres, of common-use plastic objects, and of microplastics already weathered in marine environment). This procedure can make it easier and cost-effective to process biota in monitoring studies, providing information about bioindicator/bioremediation species.

Microplastics (MPs) are insidious plastic particles with sizes ranging from 1 to 5000 µm. Their presence has been reported all over the world. Recently, bioremediation to remove MPs from water columns using filter feeders as biofilters has been proposed. In a previous lab experiment, the MP bioremediation potential of four fouling organisms from a mariculture facility (Mytilus galloprovincialis, Sabella spallanzanii, Phallusia mammillata, Paraleucilla magna) was separately assessed in single-species experiment. Herein, a follow-up of the work is presented using a multi-species approach. The four organisms were placed together in the same 5 L beaker and fed with a concentration of 250 p/L 6 µm red polystyrene discernible particles. After digesting the organisms and counting the MPs in both the water and the organisms, the results of the two experiments were compared. In the previous experiment, S. spallanzanii had the highest particle retention (PR) value (PR = 88.01%), while in this experiment, P. mammillata has the lowest PR value (PR = 31%). The multi-species approach resulted in a higher number of plastics being removed from the water (88%) compared to the single-species experiments. These fouling organisms naturally exist as a community, acting as an efficient filter with complex morphologies and hydrodynamic features. Here, this simple marine animal forest is re-evaluated by exploiting the ecosystem services provided by these organisms as a solution to MP pollution problem in a mariculture environment.

Microplastics (MPs) are dangerous and ubiquitous in the environment. The urgency to contrast plastic pollution is prompting the scientific community to offer new proposals. Recently, bioremediation using filter feeders is gaining consent as a nature-based solution. Herein four filter feeders (Mytilus galloprovincialis Lamarck, 1819, Sabella spallanzanii Gmelin, 1791, Phallusia mammillata Cuvier, 1815, Paraleucilla magna Klautau, Monteiro & Borojevic, 2004), studied in a previous laboratory experiment as MPs bioremediators, are evaluated in field conditions within 1-year. These organisms are part of an established fouling community growing on eco-friendly ropes in an Integrated Multi-Trophic Aquaculture (IMTA) in the Mar Grande of Taranto. After digesting the animal tissue, the MPs content was quantified by optical microscopy and spectroscopically characterized in the four seasons: highest values were measured in Autumn and lowest in Spring. M. galloprovincialis and P. mammillata were the most contaminated, but S. spallanzanii removed more MPs, due to its high density on the ropes. The whole community removed 3.15 × 10⁷ MPs/season, with the amount of microfibers corresponding to a bottle cap/season.

The various forms of anthropogenic pollution are regarded as a serious threat to marine coastal areas. The overproduction and mismanagement of petroleum derivatives, such as tar and plastics, have resulted in a significant correlation between these two pollutants. The aggregation of tar, microplastics (MPs), and natural materials can create plastitar blocks, which are common in coastal areas. These raise concern about the undeniable negative impact on the marine ecosystem and the associated biota, and serve as a recognizable and understandable indication of environmental decline. Here, the composition of the 11 plastitar blocks collected on the Ionian side of the Apulia region (Italy) was characterized both in tar and plastics using nuclear magnetic resonance (NMR) spectroscopy and Fourier transform infrared (FTIR) spectroscopy, respectively. Of the 250 particles extracted from the tar, 208 were identified as plastics, predominantly Polyethylene. The majority of these were in the form of pellets (90%), with fragments accounting for 5% and films and filaments representing the remaining 5%. This study provides new data that can be used to enhance the understanding of the distribution and baseline information about this novel form of pollution in Italian waters.