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Liposcelis entomophila (Psocodea: Liposcelididae) is one of the most economically important booklice in the world. This species damages stored grain and represents an allergenic health risk to humans. Because of the small size of booklice, they are difficult to identify using morphological characteristics. Traditional molecular identification, such as DNA barcoding and qRT-PCR, requires specialized equipment and takes a long time, which is not appropriate for practical usage by plant health inspectors at ports. Therefore, for storage psocid identification, we suggested a simple, rapid and relatively new technique known as loop-mediated isothermal amplification (LAMP). In our study, a new rapid molecular identification method using the LAMP technique based on the mitochondrial gene cytochrome c oxidase I (COI) was developed for L. entomophila with high specificity and efficiency. Evident amplification results could be presented if the DNA concentration was 0.001 ng/μL. Visual detection was also possible with our LAMP primers using hydroxy naphthol blue (HNB), relying on the clear change in color from violet to sky blue after amplification. This method successfully determined unknown species that were newly collected from grain stores and may facilitate L. entomophila identification in plant quarantine and stored product protection.
Lingyu Zeng; Yun Su; Vaclav Stejskal; George Opit; Radek Aulicky; Zhihong Li. Primers and visualization of LAMP: A rapid molecular identification method for Liposcelis entomophila (Enderlein) (Psocodea: Liposcelididae). Journal of Stored Products Research 2021, 93, 101855 .
AMA StyleLingyu Zeng, Yun Su, Vaclav Stejskal, George Opit, Radek Aulicky, Zhihong Li. Primers and visualization of LAMP: A rapid molecular identification method for Liposcelis entomophila (Enderlein) (Psocodea: Liposcelididae). Journal of Stored Products Research. 2021; 93 ():101855.
Chicago/Turabian StyleLingyu Zeng; Yun Su; Vaclav Stejskal; George Opit; Radek Aulicky; Zhihong Li. 2021. "Primers and visualization of LAMP: A rapid molecular identification method for Liposcelis entomophila (Enderlein) (Psocodea: Liposcelididae)." Journal of Stored Products Research 93, no. : 101855.
Glycyphagus domesticus (Acari: Glycyphagidae) is a pest mite with worldwide distribution. It damages stored products and produces allergens. Molecular diagnosis is important for the effective monitoring and control of stored-products and dust pest mites. The complete mitochondrial genome (mt genome) has become one of the most useful markers for molecular systematics, phylogenetics and diagnostics of stored-pest mites. In this study, next-generation sequencing was used to sequence the complete mt genome of G. domesticus. The complete mt genome was found to be 14,333 bp in size, containing 13 protein coding genes, 2 ribosomal RNA (rRNA) genes and 22 transfer RNA (tRNA) genes. We constructed phylogenetic trees based on concatenated thirteen protein-coding genes and two rRNAs genes. Our overall results showed that G. domesticus was found in its own separate clade from the 29 other mite species, which is likely due to the fact that this represents the first assembled mt genome of a member of the family Glycyphagidae. Overall, our study enriches the growing mt genome database for the Acariformes and will promote further studies.
Yangming Lan; Shiqian Feng; Vaclav Stejskal; Yi Wu; Radek Aulicky; Yang Cao; Zhihong Li. The complete mitochondrial genome of Glycyphagus domesticus (Acari: Glycyphagidae) using next-generation sequencing: Insight into phylogeny of Acariformes. Journal of Stored Products Research 2021, 93, 101818 .
AMA StyleYangming Lan, Shiqian Feng, Vaclav Stejskal, Yi Wu, Radek Aulicky, Yang Cao, Zhihong Li. The complete mitochondrial genome of Glycyphagus domesticus (Acari: Glycyphagidae) using next-generation sequencing: Insight into phylogeny of Acariformes. Journal of Stored Products Research. 2021; 93 ():101818.
Chicago/Turabian StyleYangming Lan; Shiqian Feng; Vaclav Stejskal; Yi Wu; Radek Aulicky; Yang Cao; Zhihong Li. 2021. "The complete mitochondrial genome of Glycyphagus domesticus (Acari: Glycyphagidae) using next-generation sequencing: Insight into phylogeny of Acariformes." Journal of Stored Products Research 93, no. : 101818.
The khapra beetle, Trogoderma granarium Everts, is defined as one of the most important quarantine pests globally, and fumigation with methyl bromide, an ozone-depleting substance, is a common phytosanitary measure currently used. The modified atmosphere (MA), irradiation, and their combination treatments of T. granarium larvae and adults were performed at room temperature (24–26 ℃) to develop an ecofriendly phytosanitary disinfestation measure and to shorten the exposure time and overcome treatment disadvantages of irradiation. Late-stage larvae are determined as the most tolerant stage resulted in large LT99.9968 values of 32.6 (29.2–37.5) and 38.0 (35.1–41.7) days treated under 1% and 2% O2 (with N2 balance) atmosphere, respectively. Ionizing radiation was used to enhance the effect of MA and the mortality was highly significantly affected by all the interaction effects, indicating that the synergistic effects present in all the combined treatments. The synergistic ratios, which is defined as the estimated lethal time for MA treatment (LD90, LD99, and LD99.9968), divided by that of combined treatment, were between 1.47 and 2.47. In the confirmatory tests, no individuals recovered from a sum of 111,366 late-stage larvae treated under 1% O2 atmosphere for 14- or 15-d after 200 Gy irradiation, which resulted in validating the probit estimations and achieving an efficacy of 99.9973% mortality at 95% confidence level. Therefore, these treatment schedules are recommended to disinfest T. granarium infecting commodities for phytosanitary purposes under the warehouse, MA packaging, or in combination with international transportation by train or sea container.
Qing-Ying Zhao; Tian-Xiu Li; Zi-Jiao Song; Tao Sun; Bo Liu; Xin Han; Zhi-Hong Li; Guo-Ping Zhan. Combination of Modified Atmosphere and Irradiation for the Phytosanitary Disinfestation of Trogoderma granarium Everts (Coleoptera: Dermestidae). Insects 2021, 12, 442 .
AMA StyleQing-Ying Zhao, Tian-Xiu Li, Zi-Jiao Song, Tao Sun, Bo Liu, Xin Han, Zhi-Hong Li, Guo-Ping Zhan. Combination of Modified Atmosphere and Irradiation for the Phytosanitary Disinfestation of Trogoderma granarium Everts (Coleoptera: Dermestidae). Insects. 2021; 12 (5):442.
Chicago/Turabian StyleQing-Ying Zhao; Tian-Xiu Li; Zi-Jiao Song; Tao Sun; Bo Liu; Xin Han; Zhi-Hong Li; Guo-Ping Zhan. 2021. "Combination of Modified Atmosphere and Irradiation for the Phytosanitary Disinfestation of Trogoderma granarium Everts (Coleoptera: Dermestidae)." Insects 12, no. 5: 442.
The Ceratitis FARQ complex (formerly FAR complex) includes four frugivorous tephritids, Ceratitis fasciventris, C. anonae, C. rosa and C. quilicii, the latter two causing important agricultural losses in Africa. Although FARQ species can be identified on the basis of subtle morphological differences, they cannot be resolved as monophyletic when trying phylogenetic tree reconstructions based on mitochondrial or nuclear gene fragments except for microsatellites. In this study, we used mitogenome and genome-wide SNPs to investigate the phylogenetic relationship within the complex as well as between all four Ceratitis subgenera. The analysis of 13 species supported the monophyly of the Ceratitis subgenera Ceratitis, Ceratalaspis, Pardalaspis, and recovered Pterandrus as paraphyletic but could not properly resolve species within the FARQ complex. Conversely, gene and species tree reconstructions based on 785,484 genome-wide SNPs could consistently resolve the FARQ taxa and provide insights into their phylogenetic relationships. Gene flow was detected by TreeMix analysis from C. quilicii to C. fasciventris, suggesting the existence of introgression events in the FARQ complex. Our results suggest that genome-wide SNPs represent a suitable tool for the molecular diagnosis of FARQ species and could possibly be used to develop rapid diagnostic methods or to trace the origins of intercepted samples.
Yue Zhang; Marc De Meyer; Massimiliano Virgilio; Shiqian Feng; Kemo Badji; Zhihong Li. Phylogenomic resolution of the Ceratitis FARQ complex (Diptera: Tephritidae). Molecular Phylogenetics and Evolution 2021, 161, 107160 .
AMA StyleYue Zhang, Marc De Meyer, Massimiliano Virgilio, Shiqian Feng, Kemo Badji, Zhihong Li. Phylogenomic resolution of the Ceratitis FARQ complex (Diptera: Tephritidae). Molecular Phylogenetics and Evolution. 2021; 161 ():107160.
Chicago/Turabian StyleYue Zhang; Marc De Meyer; Massimiliano Virgilio; Shiqian Feng; Kemo Badji; Zhihong Li. 2021. "Phylogenomic resolution of the Ceratitis FARQ complex (Diptera: Tephritidae)." Molecular Phylogenetics and Evolution 161, no. : 107160.
Oriental fruit fly, Bactrocera dorsalis (Hendel), is a destructive and highly polyphagous invasive fruit fly species of numerous fruit crops in global agriculture. Population genetic structure of this species from five different locations of Bangladesh was examined with other samples (collected from GenBank) from 15 sites of neighboring Asian countries. A fragment of 770 bp mitochondrial DNA cox1 was used to investigate the genetic diversity and the relationship between genetic patterns and geographical distribution of B. dorsalis. A total of 232 variable sites (33.23% of the 698 bp aligned consensus sequences) and 419 unique haplotypes were identified from 710 individuals. Indices of genetic diversity suggested that without exclusion from geographical areas, B. dorsalis retained a relatively high degree of genetic diversity. A demographic assessment [Tajimas’ D test, Fu’s Fs test and sum of square deviation (SSD values)] revealed that both current and historical variables performed a significant role in deciding the weak genetic structure with some exceptions. In Bangladesh, high levels of genetic diversity with a weak genetic structure indicated that the severity of this pest might increase in the future. Proper management techniques should be taken to overcome the future severity of this kind of destructive insect.
Sultana Afroz; Shibly Noman; Yue Zhang; Yujia Qin; S.M. Kamrul Hasan Chowdhury; Zhihong Li. Population genetic structure of Bactrocera dorsalis based on cox1 sequences from Bangladesh and neighboring countries. Journal of Asia-Pacific Entomology 2021, 24, 182 -190.
AMA StyleSultana Afroz, Shibly Noman, Yue Zhang, Yujia Qin, S.M. Kamrul Hasan Chowdhury, Zhihong Li. Population genetic structure of Bactrocera dorsalis based on cox1 sequences from Bangladesh and neighboring countries. Journal of Asia-Pacific Entomology. 2021; 24 (2):182-190.
Chicago/Turabian StyleSultana Afroz; Shibly Noman; Yue Zhang; Yujia Qin; S.M. Kamrul Hasan Chowdhury; Zhihong Li. 2021. "Population genetic structure of Bactrocera dorsalis based on cox1 sequences from Bangladesh and neighboring countries." Journal of Asia-Pacific Entomology 24, no. 2: 182-190.
Dermestid beetles (Coleoptera: Dermestidae) are important pests of various stored products, posing potential threats to international trade. Their detailed characterization on molecular basis is a pre-requisite for proper identification and for understanding of their phylogenetic relationships. In this work, the whole mitochondrial genomes (mitogenomes) of Trogoderma granarium, Dermestes lardarius, D. ater, Attagenus augustatus augustatus and Attagenus unicolor japonicus were firstly sequenced to update the database using the next-generation sequencing technique. Based on the selected model species, a comparative analysis of four Dermestidae genera was performed. The mitochondrial genomes of these five species above showed high similarity in nucleotide composition, base composition and gene order, including 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs) and a non-coding control region, which was similar to most of Coleoptera species. The phylogenetic analysis based on the PCGs and two rRNAs indicated that the relationships within Dermestidae were reconstructed as (((Trogoderma + Anthrenus) + Attagenus) + Dermestes) using both Maximum Likelihood (ML) and Bayesian Inference (BI) analysis. However, more mitogenomes should be sequenced to obtain a more holistic view of the whole family. This study not only showed the mitogenomes of five Dermestidae species and their high conservativeness, but also discussed its implications for reconstructing a more comprehensive phylogeny of dermestids.
Lingyu Zeng; Youting Pang; Shiqian Feng; Yuning Wang; Vaclav Stejskal; Radek Aulicky; Shengfang Zhang; Zhihong Li. Comparative mitochondrial genomics of five Dermestid beetles (Coleoptera: Dermestidae) and its implications for phylogeny. Genomics 2020, 113, 927 -934.
AMA StyleLingyu Zeng, Youting Pang, Shiqian Feng, Yuning Wang, Vaclav Stejskal, Radek Aulicky, Shengfang Zhang, Zhihong Li. Comparative mitochondrial genomics of five Dermestid beetles (Coleoptera: Dermestidae) and its implications for phylogeny. Genomics. 2020; 113 (1):927-934.
Chicago/Turabian StyleLingyu Zeng; Youting Pang; Shiqian Feng; Yuning Wang; Vaclav Stejskal; Radek Aulicky; Shengfang Zhang; Zhihong Li. 2020. "Comparative mitochondrial genomics of five Dermestid beetles (Coleoptera: Dermestidae) and its implications for phylogeny." Genomics 113, no. 1: 927-934.
In this study, the complete mitochondrial genome of Dermestes dimidiatus ab. rosea was characterized using next-generation sequencing, and the phylogenetic relationships of superfamily Bostrichoidea were established. The results showed that the mitochondrial genome of D. dimidiatus ab. rosea was 16,073 bp in size, and it contained thirteen protein-coding genes (PCGs), twenty-two transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a control region. The composition of the whole mitochondrial genome of this species was 41.3% A, 13.5% C, 9.3% G, and 35.9% T, which had high A + T content (77.2%). Phylogenetic relationships of the superfamily Bostrichoidea showed that D. dimidiatus ab. rosea and Dermestes tessellatocollis formed in a clade that was a sister group to (Dermestes maculatus + Dermestes frischii), indicating that Dermestidae was a monophyletic group. This is the first report of a complete mitochondrial genome of D. dimidiatus ab. rosea and preliminary study of Bostrichoidea mitochondrial genome, which is of great significance for the molecular identification of this species and the enrichment of mitochondrial genome database.
Yuning Wang; Yue Zhang; Shuhua Wei; Farman Ullah; Youting Pang; Chengcai Ma; Zhihong Li. The first complete mitochondrial genome of Dermestes dimidiatus ab. rosea Kusnezova and its phylogenetic implications for the superfamily Bostrichoidea. Mitochondrial DNA Part B 2020, 5, 3805 -3807.
AMA StyleYuning Wang, Yue Zhang, Shuhua Wei, Farman Ullah, Youting Pang, Chengcai Ma, Zhihong Li. The first complete mitochondrial genome of Dermestes dimidiatus ab. rosea Kusnezova and its phylogenetic implications for the superfamily Bostrichoidea. Mitochondrial DNA Part B. 2020; 5 (4):3805-3807.
Chicago/Turabian StyleYuning Wang; Yue Zhang; Shuhua Wei; Farman Ullah; Youting Pang; Chengcai Ma; Zhihong Li. 2020. "The first complete mitochondrial genome of Dermestes dimidiatus ab. rosea Kusnezova and its phylogenetic implications for the superfamily Bostrichoidea." Mitochondrial DNA Part B 5, no. 4: 3805-3807.
The adverse effects of invasive pests on ecosystems have gradually intensified, and the prevention of invasive pests is a long-standing research focus. Genetic control strategies are effective, sustainable and environmentally friendly methods for controlling pests and have received substantial attention worldwide. However, there is a lack of researches on the genetic control of Bactrocera dorsalis Hendel, a global invasive pest with strong flight ability. The wing of B. dorsalis as an important flight organ may be a main reason for its widespread occurrence. Here, we first analysed the wing structure of B. dorsalis and found that its wing has the typical characteristic of Diptera insect wing. The spatiotemporal expressions of the wing development genes were examined by quantitative real-time PCR, antibody staining and RNA in situ hybridization. The results indicated that the wing development genes were significantly upregulated in the pupal stage, and the regional expression of each gene was clarified. Wingless (wg), a key gene that significantly affects wing development, was selected from nine genes through RNA interference and used to simulate the field control of B. dorsalis. The offspring population and the fruit-borer rate decreased significantly after the simulated control. This study provides preliminary support for the application of genetic pest control by regulating the wing development gene and proposes a novel idea for solving the problem of the extensive spread of B. dorsalis.
Shaokun Guo; Xiaoyu Guo; Linyu Zheng; Zihua Zhao; Lijun Liu; Jie Shen; Zhihong Li. A potential genetic control by suppression of the wing developmental gene wingless in a global invasive pest Bactrocera dorsalis. Journal of Pest Science 2020, 94, 517 -529.
AMA StyleShaokun Guo, Xiaoyu Guo, Linyu Zheng, Zihua Zhao, Lijun Liu, Jie Shen, Zhihong Li. A potential genetic control by suppression of the wing developmental gene wingless in a global invasive pest Bactrocera dorsalis. Journal of Pest Science. 2020; 94 (2):517-529.
Chicago/Turabian StyleShaokun Guo; Xiaoyu Guo; Linyu Zheng; Zihua Zhao; Lijun Liu; Jie Shen; Zhihong Li. 2020. "A potential genetic control by suppression of the wing developmental gene wingless in a global invasive pest Bactrocera dorsalis." Journal of Pest Science 94, no. 2: 517-529.
(Coleoptera: Laemophloeidae) are economically important pests, which damage stored products in granaries and mills. Because it is difficult to distinguish Cryptolestes species morphologically, we develop here a high sensitivity real-time PCR method for distinguishing five species of Cryptolestes (C. ferrugineus, C. pusillus, C. turcicus, C. pusilloides and C. capensis). The lower limit of DNA concentration required for species identification is 0.1 ng/μl for C. ferruginues and C. pusilloides, and 0.01 ng/μl for C. pusillus, C. turcicus, and C. capensis. This method successfully determined species assignments for previously unidentified Cryptolestes spp. Collected from the field and will facilitate Cryptolestes identification in the field of plant quarantine and stored product protection.
Dongxu Chen; Tao Zhang; Radek Aulicky; Vaclav Stejskal; Yonglin Ren; Yang Cao; David Hawthorne; Zhihong Li. Real-time PCR for identification of five species of Cryptolestes based on COI barcode region. Journal of Stored Products Research 2020, 87, 101623 .
AMA StyleDongxu Chen, Tao Zhang, Radek Aulicky, Vaclav Stejskal, Yonglin Ren, Yang Cao, David Hawthorne, Zhihong Li. Real-time PCR for identification of five species of Cryptolestes based on COI barcode region. Journal of Stored Products Research. 2020; 87 ():101623.
Chicago/Turabian StyleDongxu Chen; Tao Zhang; Radek Aulicky; Vaclav Stejskal; Yonglin Ren; Yang Cao; David Hawthorne; Zhihong Li. 2020. "Real-time PCR for identification of five species of Cryptolestes based on COI barcode region." Journal of Stored Products Research 87, no. : 101623.
It is acknowledged that human activities have initiated and increased steppe habitat loss and fragmentation in Northwest China. Carabid beetles are often used as bioindicators of environmental change because they are extremely sensitive to disturbance. We chose 42 landscapes (18 fragmented and 24 continuous) in two steppe types in Ningxia, Northwest China, to examine the influence of steppe fragmentation on carabid beetles. The results showed that the canonical correlation coefficient between carabid beetles and landscape fragmentation was greatest at a 7-km spatial scale in both desert and typical steppes. Furthermore, the response of carabid beetles to steppe fragmentation was species-specific in both desert and typical steppes. Steppe fragmentation in the desert steppe could significantly enhance the richness and abundance of carabid beetles, which contrasts with the typical steppe. Additionally, steppe fragmentation significantly increased the abundance of two dominant carabid beetles in the desert steppe only. Therefore, the effects of steppe fragmentation on carabid beetles were species-dependent and were also mediated by the plant community and microclimate in the different steppes. The redundancy analysis (RDA) indicated that plant richness and cover were important factors affecting carabid diversity. Finally, these results provide experimental evidence and technical data for biodiversity conservation management in the steppes of Northwest China.
Shuhua Wei; Wenguang Huang; Mengmeng Zhu; Liyuan Gao; Ying Wang; Rong Zhang; Zhihong Li; Zihua Zhao. The asymmetric responses of carabid beetles to steppe fragmentation in Northwest China. Global Ecology and Conservation 2020, 23, e01058 .
AMA StyleShuhua Wei, Wenguang Huang, Mengmeng Zhu, Liyuan Gao, Ying Wang, Rong Zhang, Zhihong Li, Zihua Zhao. The asymmetric responses of carabid beetles to steppe fragmentation in Northwest China. Global Ecology and Conservation. 2020; 23 ():e01058.
Chicago/Turabian StyleShuhua Wei; Wenguang Huang; Mengmeng Zhu; Liyuan Gao; Ying Wang; Rong Zhang; Zhihong Li; Zihua Zhao. 2020. "The asymmetric responses of carabid beetles to steppe fragmentation in Northwest China." Global Ecology and Conservation 23, no. : e01058.
Liposcelis corrodens (Psocodea: Liposcelididae) is recognized as one species of common stored product pests, however, it has not been reported in China. In this study, we identified Liposcelis specimens collected from a maize reserve depot in China as Liposcelis corrodens based on morphological and molecular methods. The identification of L. corrodens and keys to the stored Liposcelis species of Group IID were reported. The mitochondrial gene cytochrome c oxidase I (mtDNA COI) of the specimens was sequenced and used as a DNA barcode to conduct molecular identification and phylogenetic analyses with other Liposcelis species of Group IID from different geographical populations. This is the first record of L. corrodens in China and it will be useful for the further studies of taxonomy and management of stored booklice.
Jixiang Cui; Yun Su; Shiqian Feng; Peng Wei; Xingyue Liu; Zhihong Li. Morphological and molecular identification of Liposcelis corrodens (Heymons, 1909) (Psocodea: Liposcelididae) as the first record from China. Journal of Stored Products Research 2020, 87, 101588 .
AMA StyleJixiang Cui, Yun Su, Shiqian Feng, Peng Wei, Xingyue Liu, Zhihong Li. Morphological and molecular identification of Liposcelis corrodens (Heymons, 1909) (Psocodea: Liposcelididae) as the first record from China. Journal of Stored Products Research. 2020; 87 ():101588.
Chicago/Turabian StyleJixiang Cui; Yun Su; Shiqian Feng; Peng Wei; Xingyue Liu; Zhihong Li. 2020. "Morphological and molecular identification of Liposcelis corrodens (Heymons, 1909) (Psocodea: Liposcelididae) as the first record from China." Journal of Stored Products Research 87, no. : 101588.
Citrus sinensis (L.) Osbeck is an important economic product in South China, but the presence of quarantine pests in this product proposes the potential threat to international trade security. To find a proper phytosanitary cold treatment for Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), commonly called oriental fruit fly, one of the most serious quarantine insects in navel orange, eggs in petri dish and larvae in navel orange fruits were exposed to a 1.7 °C cold chamber for 0–11 days to compare the tolerance to cold treatment. The 2nd instar larva (4 days) is the most tolerant stage, and the estimated time for 99.9968% mortality at the 95% confidence level is 11.3 (9.5, 14.6) days. Then 15 days was selected as the target time for the confirmatory tests, resulting in no survivors from 37,792 treated larvae with the efficacy of 99.9921% mortality at the 95% confidence level. The quality assessments were conducted to compare the effect on the navel orange fruit between cold treatment and the conventional cold storage. Results indicated that the cold treatment did not negatively affect the fruit quality. Therefore, this cold treatment showed potential as a commercial quarantine treatment for navel orange in international trade.
Yan Fang; Fenfen Kang; Guoping Zhan; Chen Ma; Yuguang Li; Lin Wang; Yadong Wei; Xiwu Gao; Zhihong Li; Yuejin Wang. The Effects of a Cold Disinfestation on Bactrocera dorsalis Survival and Navel Orange Quality. Insects 2019, 10, 452 .
AMA StyleYan Fang, Fenfen Kang, Guoping Zhan, Chen Ma, Yuguang Li, Lin Wang, Yadong Wei, Xiwu Gao, Zhihong Li, Yuejin Wang. The Effects of a Cold Disinfestation on Bactrocera dorsalis Survival and Navel Orange Quality. Insects. 2019; 10 (12):452.
Chicago/Turabian StyleYan Fang; Fenfen Kang; Guoping Zhan; Chen Ma; Yuguang Li; Lin Wang; Yadong Wei; Xiwu Gao; Zhihong Li; Yuejin Wang. 2019. "The Effects of a Cold Disinfestation on Bactrocera dorsalis Survival and Navel Orange Quality." Insects 10, no. 12: 452.
Tephritidae fruit flies (Diptera: Tephritidae) are regarded as important damage-causing species due to their ability to cause great economic losses in fruit and vegetable crops. Bactrocera minax and Bactrocera tsuneonis are two sibling species of the subgenus Tetradacus of Bactrocera that are distributed across a limited area of China, but have caused serious impacts. They share similar morphological characteristics. These characteristics can only be observed in the female adult individuals. The differences between them cannot be observed in preimaginal stages. Thus, it is difficult to distinguish them in preimaginal stages morphologically. In this study, we used molecular diagnostic methods based on cytochrome c oxidase subunit I and species-specific markers to identify these two species and improve upon the false-positive results of previous species-detection primers. DNA barcode sequences were obtained from 900 individuals of B. minax and 63 individuals of B. tsuneonis. Based on these 658 bp DNA barcode sequences of the cytochrome c oxidase subunit I gene, we successfully designed the species-specific primers for B. minax and B. tsuneonis. The size of the B. minax specific fragment was 422 bp and the size of the B. tsuneonis specific fragment was 456 bp. A series of PCR trials ensured the specificity of these two pairs of primers. Sensitivity assay results demonstrated that the detection limit for the DNA template concentration was 0.1~1 ng/μL for these two species. In this study, we established a more reliable, rapid, and low-cost molecular identification method for all life stages of B. minax and B. tsuneonis. Species-specific PCR can be applied in plant quarantine, monitoring and control of B. minax and B. tsuneonis.
Linyu Zheng; Yue Zhang; Wenzhao Yang; Yiying Zeng; Fan Jiang; Yujia Qin; Jiafeng Zhang; Zhaochun Jiang; Wenzhao Hu; Dijin Guo; Jia Wan; Zihua Zhao; Lijun Liu; Zhihong Li. New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes. Insects 2019, 10, 447 .
AMA StyleLinyu Zheng, Yue Zhang, Wenzhao Yang, Yiying Zeng, Fan Jiang, Yujia Qin, Jiafeng Zhang, Zhaochun Jiang, Wenzhao Hu, Dijin Guo, Jia Wan, Zihua Zhao, Lijun Liu, Zhihong Li. New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes. Insects. 2019; 10 (12):447.
Chicago/Turabian StyleLinyu Zheng; Yue Zhang; Wenzhao Yang; Yiying Zeng; Fan Jiang; Yujia Qin; Jiafeng Zhang; Zhaochun Jiang; Wenzhao Hu; Dijin Guo; Jia Wan; Zihua Zhao; Lijun Liu; Zhihong Li. 2019. "New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes." Insects 10, no. 12: 447.
It is well known that human activities and climate change have increased steppe habitat loss and fragmentation in Northwest China. Carabid beetles are often used as bioindicators of environmental change because they are extremely sensitive to disturbance. We chose 42 landscapes (18 fragmented and 24 continuous) in both desert and typical steppes of Northwest China to examine the influence of habitat loss and fragmentation on carabid beetle communities. The results showed the largest correlation coefficient between carabid communities and landscape compositions within a 7-km spatial scale in both desert and typical steppes. Further, the response of carabid communities to habitat fragmentation was species-specific in both desert and typical steppes. Habitat fragmentation in the desert steppe had positive effects on the richness and abundance of carabid communities, while in the typical steppe, the effects were negative. Additionally, habitat fragmentation significantly decreased the abundance of two common carabid species in the desert steppe. Therefore, the effects of habitat fragmentation on carabid biodiversity differ with species characteristics and habitat traits, where plant communities, soil structure, and microclimate vary in the different steppe types. The results of this study provide experimental evidence and technical support for biodiversity conservation management in the steppes of Northwest China.
Shu-Hua Wei; Wen-Guang Huang; Meng-Meng Zhu; Li-Yuan Gao; Ying Wang; Rong Zhang; Zhi-Hong Li; Zi-Hua Zhao. Habitat Fragmentation Effects on Community Composition of Carabid in Steppes of Northwest China. 2019, 1 .
AMA StyleShu-Hua Wei, Wen-Guang Huang, Meng-Meng Zhu, Li-Yuan Gao, Ying Wang, Rong Zhang, Zhi-Hong Li, Zi-Hua Zhao. Habitat Fragmentation Effects on Community Composition of Carabid in Steppes of Northwest China. . 2019; ():1.
Chicago/Turabian StyleShu-Hua Wei; Wen-Guang Huang; Meng-Meng Zhu; Li-Yuan Gao; Ying Wang; Rong Zhang; Zhi-Hong Li; Zi-Hua Zhao. 2019. "Habitat Fragmentation Effects on Community Composition of Carabid in Steppes of Northwest China." , no. : 1.
Dacus bivittatus and Dacus ciliatus are destructive pests of Cucurbitaceae crops including cucumber, zucchini and melons. Recent molecular phylogenetic studies conflicted with morphological taxonomy regarding relationships between Bactrocera, Dacus and Zeugodacus. In this study, we sequenced the complete mitochondrial genomes of the above species which are representatives of two subgenera of Dacus (Dacus and Didacus) not previously sequenced and reconstructed the phylogeny of Tephritidae. The mitochondrial genomes of D. bivittatus and D. ciliatus were 15,833 bp and 15,808 bp in length, respectively. The 37 genes, including 13 protein-coding genes (PCGs), 2 rRNA genes and 22 tRNA genes, with a long non-coding region (A + T-rich control region) were in the same arrangement as the ancestral insect mitochondrial genome. Phylogenetic analysis showed that Dacus has a closer relationship of Zeugodacus rather than Bactrocera. Our phylogenetic results further support the recent proposals that Zeugodacus should be considered as a genus not a subgenus of Bactrocera. Whole mitochondrial genomes of D. bivittatus and D. ciliatus could be useful in further studies for species diagnosis, evolution and phylogeny research within Tephritidae.
Yue Zhang; Shiqian Feng; Lida Fekrat; Fan Jiang; Matodzi Khathutshelo; Zhihong Li. The first two complete mitochondrial genome of Dacus bivittatus and Dacus ciliatus (Diptera: Tephritidae) by next-generation sequencing and implications for the higher phylogeny of Tephritidae. International Journal of Biological Macromolecules 2019, 140, 469 -476.
AMA StyleYue Zhang, Shiqian Feng, Lida Fekrat, Fan Jiang, Matodzi Khathutshelo, Zhihong Li. The first two complete mitochondrial genome of Dacus bivittatus and Dacus ciliatus (Diptera: Tephritidae) by next-generation sequencing and implications for the higher phylogeny of Tephritidae. International Journal of Biological Macromolecules. 2019; 140 ():469-476.
Chicago/Turabian StyleYue Zhang; Shiqian Feng; Lida Fekrat; Fan Jiang; Matodzi Khathutshelo; Zhihong Li. 2019. "The first two complete mitochondrial genome of Dacus bivittatus and Dacus ciliatus (Diptera: Tephritidae) by next-generation sequencing and implications for the higher phylogeny of Tephritidae." International Journal of Biological Macromolecules 140, no. : 469-476.
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been widely applied in functional genomics research and plant breeding. In contrast to the off-target studies of mammalian cells, there is little evidence for the common occurrence of off-target sites in plants and a great need exists for accurate detection of editing sites. Here, we summarized the precision of CRISPR/Cas9-mediated mutations for 281 targets and found that there is a preference for single nucleotide deletions/insertions and longer deletions starting from 40 nt upstream or ending at 30 nt downstream of the cleavage site, which suggested the candidate sequences for editing sites detection by whole-genome sequencing (WGS). We analyzed the on-/off-target sites of 6 CRISPR/Cas9-mediated Arabidopsis plants by the optimized method. The results showed that the on-target editing frequency ranged from 38.1% to 100%, and one off target at a frequency of 9.8%-97.3% cannot be prevented by increasing the specificity or reducing the expression level of the Cas9 enzyme. These results indicated that designing guide RNA with high specificity may be the preferred factor to avoid the off-target events, and it is necessary to predict or detect off-target sites by WGS-based methods for preventing off targets caused by genome differences in different individuals.
Wenjie Xu; Wei Fu; Zhu; Zhihong Li; Wang; Yongjiang Zhang; Pengyu Zhu; Chenguang Wang; Chaonan Wang; Shuifang Zhu; Xu; Fu; Li. Comprehensive Analysis of CRISPR/Cas9-Mediated Mutagenesis in Arabidopsis thaliana by Genome-wide Sequencing. International Journal of Molecular Sciences 2019, 20, 4125 .
AMA StyleWenjie Xu, Wei Fu, Zhu, Zhihong Li, Wang, Yongjiang Zhang, Pengyu Zhu, Chenguang Wang, Chaonan Wang, Shuifang Zhu, Xu, Fu, Li. Comprehensive Analysis of CRISPR/Cas9-Mediated Mutagenesis in Arabidopsis thaliana by Genome-wide Sequencing. International Journal of Molecular Sciences. 2019; 20 (17):4125.
Chicago/Turabian StyleWenjie Xu; Wei Fu; Zhu; Zhihong Li; Wang; Yongjiang Zhang; Pengyu Zhu; Chenguang Wang; Chaonan Wang; Shuifang Zhu; Xu; Fu; Li. 2019. "Comprehensive Analysis of CRISPR/Cas9-Mediated Mutagenesis in Arabidopsis thaliana by Genome-wide Sequencing." International Journal of Molecular Sciences 20, no. 17: 4125.
Climate change is a major factor driving shifts in the distribution of invasive pests. The oriental fruit fly, Bactrocera dorsalis, native to mainland Asia, has spread throughout Southeast Asia and sub-Saharan Africa. Recently, the species has extended its Asian range northward into regions previously thought unsuitable which presents a major new risk to temperate zone agriculture and has invaded Italy. Thus, it is necessary to study how climate change may impact on the global distribution of B. dorsalis. MaxEnt models were used to map suitable habitat for this species under current and future climate conditions averaged from four global climate models under two representative emission pathways in 2050 and 2070. The results highlighted that a total of 30.84% of the world’s land mass is currently climatically suitable including parts of the western coast and southeast of the USA, most of Latin America, parts of Mediterranean coastal European regions, northern and coastal Australia, and the north island of New Zealand. Under future climate conditions, the risk area of B. dorsalis in the northern hemisphere was projected to expand northward, while in the southern hemisphere, it would be southward, especially by 2070 under RCP85 with very high greenhouse gas emissions. Future management of this pest should consider the impacts of the global climate change on its potential geographical distribution.
Yujia Qin; Cong Wang; Zihua Zhao; Xubin Pan; Zhihong Li. Climate change impacts on the global potential geographical distribution of the agricultural invasive pest, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Climatic Change 2019, 155, 145 -156.
AMA StyleYujia Qin, Cong Wang, Zihua Zhao, Xubin Pan, Zhihong Li. Climate change impacts on the global potential geographical distribution of the agricultural invasive pest, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Climatic Change. 2019; 155 (2):145-156.
Chicago/Turabian StyleYujia Qin; Cong Wang; Zihua Zhao; Xubin Pan; Zhihong Li. 2019. "Climate change impacts on the global potential geographical distribution of the agricultural invasive pest, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)." Climatic Change 155, no. 2: 145-156.
Low temperatures play an important role in arthropods because they affect both the individual and population development of all physiological and behavioural activities. Manipulation with low temperatures is a primary nonchemical pest control method. For stored product and food industry practitioners, a knowledge of pest thermal requirements, in particular threshold temperatures at which development and other activities of a particular pest species cease, is of crucial importance. This review presents summary data regarding the lower temperature thresholds of 121 species of stored product and food industry pests from six arthropod taxa (Acari, Coleoptera, Lepidoptera, Psocoptera, Diptera, and Blattodea). In particular, this review collected and summarized information regarding the lower development thresholds, lower population thresholds, lower acoustic or respiratory thresholds, lower walking and flying thresholds and lower trap capture thresholds for flying and walking arthropods. The average lower development threshold (LDT) differed among orders: the lowest was reported for Acari (6.8 °C) and Diptera (8.1 °C), followed by Lepidoptera (11.3 °C) and Psocoptera (13.8 °C), and the highest was reported for Coleoptera (14 °C) and Blattodea (15 °C). An exclusion-function was established showing the percentage of pest species (n = 112) that were developmentally suppressed (excluded) due to temperatures reaching the LDT in the range of decreasing temperatures from 25 °C to 0 °C. We scaled various temperature thresholds from the lowest to highest temperature as follows: the walking threshold, the trap capture threshold for walking insects, the lower development threshold, lower population threshold, lower flying threshold and the lower trap capture threshold for flying pests. Important pest species were identified for which information regarding the lower temperature threshold is missing, or for which the information is too variable and should be refined in future research.
Vaclav Stejskal; Tomas Vendl; Zhihong Li; Radek Aulicky; Zhiong Li. Minimal Thermal Requirements for Development and Activity of Stored Product and Food Industry Pests (Acari, Coleoptera, Lepidoptera, Psocoptera, Diptera and Blattodea): A Review. Insects 2019, 10, 149 .
AMA StyleVaclav Stejskal, Tomas Vendl, Zhihong Li, Radek Aulicky, Zhiong Li. Minimal Thermal Requirements for Development and Activity of Stored Product and Food Industry Pests (Acari, Coleoptera, Lepidoptera, Psocoptera, Diptera and Blattodea): A Review. Insects. 2019; 10 (5):149.
Chicago/Turabian StyleVaclav Stejskal; Tomas Vendl; Zhihong Li; Radek Aulicky; Zhiong Li. 2019. "Minimal Thermal Requirements for Development and Activity of Stored Product and Food Industry Pests (Acari, Coleoptera, Lepidoptera, Psocoptera, Diptera and Blattodea): A Review." Insects 10, no. 5: 149.
Booklice in the genus Liposcelis (Psocodea: Liposcelididae) are essential storage pests worldwide. Fragmented mt genomes have been identified in the Liposcelis species together with the typical mitochondrial (mt) genome, which is a single circular chromosome with 37 genes. Gene rearrangement, pseudogenes, and repeat regions (RRs) are very common among fragmented mt genomes. We sequenced the mt genome of the booklouse L. brunnea, the type species of the genus Liposcelis. We identified 37 genes in the mt genome of L. brunnea, which was fragmented into three chromosomes. The chromosomes I, II, III were 7.3 kb, 5.5 kb, and 5.3 kb in size with 9, 19, and 15 genes, respectively. In addition, 16 pseudogenes and four repeat regions were present in three chromosomes. Gene rearrangement in the mt genome of L. brunnea was obvious compared to that in other mt genomes in the genus Liposcelis. We found a possible correlation among mt genome rearrangement, the morphological classification standard, and phylogenetic relationships. In summary, a three-chromosome mt genome in an insect was identified for the first time, which may aid in understanding mt genome fragmentation, gene rearrangement, and evolution.
Shiqian Feng; Hu Li; Fan Song; Yannan Wang; Vaclav Stejskal; Wanzhi Cai; Zhihong Li. A novel mitochondrial genome fragmentation pattern in Liposcelis brunnea, the type species of the genus Liposcelis (Psocodea: Liposcelididae). International Journal of Biological Macromolecules 2019, 132, 1296 -1303.
AMA StyleShiqian Feng, Hu Li, Fan Song, Yannan Wang, Vaclav Stejskal, Wanzhi Cai, Zhihong Li. A novel mitochondrial genome fragmentation pattern in Liposcelis brunnea, the type species of the genus Liposcelis (Psocodea: Liposcelididae). International Journal of Biological Macromolecules. 2019; 132 ():1296-1303.
Chicago/Turabian StyleShiqian Feng; Hu Li; Fan Song; Yannan Wang; Vaclav Stejskal; Wanzhi Cai; Zhihong Li. 2019. "A novel mitochondrial genome fragmentation pattern in Liposcelis brunnea, the type species of the genus Liposcelis (Psocodea: Liposcelididae)." International Journal of Biological Macromolecules 132, no. : 1296-1303.
Many insects have the capacity to increase their resistance to high temperatures by undergoing heat hardening at non‐lethal temperatures. Although this response is well established, its molecular underpinnings has only been investigated in a few species where it seems to relate at least partly to the expression of heat shock protein (Hsp) genes. Here we studied the mechanism of hardening and associated transcription responses in larvae of two invasive fruit fly species in China, Bactrocera dorsalis and Bactrocera correcta. Both species showed hardening which increased resistance to 45 °C, although the more widespread B. dorsalis hardened better at higher temperatures compared to B. correcta which hardened better at lower temperatures. Transcriptional analyses highlighted expression changes in a number of genes representing different biochemical pathways, but these changes and pathways were inconsistent between the two species. Overall B. dorsalis showed expression changes in more genes than B. correcta. Hsp genes tended to be upregulated at a hardening temperature of 38 °C in both species, while at 35°C many Hsp genes tended to be upregulated in B. correcta but not B. dorsalis. One candidate gene (the small heat shock protein gene, Hsp23) with a particularly high level of up‐regulation was investigated functionally using RNA interference (RNAi). We found that RNAi may be more efficient in B. dorsalis, in which suppression of the expression of this gene removed the hardening response, whereas in B. correcta RNAi did not decrease the hardening response. The different patterns of gene expression in these two species at the two hardening temperatures highlight the diverse mechanisms underlying hardening even in closely related species. These results may provide target genes for future control efforts against such pest species. This article is protected by copyright. All rights reserved.
Xinyue Gu; Yan Zhao; Yun Su; Jiajiao Wu; Ziya Wang; Juntao Hu; Lijun Liu; Zihua Zhao; Ary A. Hoffmann; Bing Chen; Zhihong Li. A transcriptional and functional analysis of heat hardening in two invasive fruit fly species, Bactrocera dorsalis and Bactrocera correcta. Evolutionary Applications 2019, 12, 1147 -1163.
AMA StyleXinyue Gu, Yan Zhao, Yun Su, Jiajiao Wu, Ziya Wang, Juntao Hu, Lijun Liu, Zihua Zhao, Ary A. Hoffmann, Bing Chen, Zhihong Li. A transcriptional and functional analysis of heat hardening in two invasive fruit fly species, Bactrocera dorsalis and Bactrocera correcta. Evolutionary Applications. 2019; 12 (6):1147-1163.
Chicago/Turabian StyleXinyue Gu; Yan Zhao; Yun Su; Jiajiao Wu; Ziya Wang; Juntao Hu; Lijun Liu; Zihua Zhao; Ary A. Hoffmann; Bing Chen; Zhihong Li. 2019. "A transcriptional and functional analysis of heat hardening in two invasive fruit fly species, Bactrocera dorsalis and Bactrocera correcta." Evolutionary Applications 12, no. 6: 1147-1163.