Despite great advances in the treatment of oncological diseases, the development of medical technologies to prevent or reduce complications of therapy, in particular, those associated with surgery and the introduction of antibiotics, remains relevant. The aim of this study is to evaluate the effectiveness of the use of autoprobiotics based on indigenous non-pathogenic strains of Enterococcus faecium and Enterococcus hirae as a personalized functional food product (PFFP) in the complex therapy of colorectal cancer (CRC) in the early postoperative period. A total of 36 patients diagnosed with CRC were enrolled in the study. Study group A comprised 24 CRC patients who received autoprobiotic therapy in the early postoperative period, while the control group C included 12 CRC patients without autoprobiotic therapy. Prior to surgery and between days 14 and 16 post-surgery, comprehensive evaluations were conducted on all patients, encompassing the following: stool and gastroenterological complaints analysis, examination of the gut microbiota (bacteriological study, quantitative polymerase chain reaction, metagenome analysis), and analysis of interleukins in the serum. Results: The use of autoprobiotics led to a decrease in dyspeptic complaints after surgery. It was also associated with the absence of postoperative complications, did not cause any side effects, and led to a decrease in the level of pro-inflammatory cytokines (IL-6 and IL-18) in the blood serum. The use of autoprobiotics led to positive changes in the structure of escherichia and enterococci populations, the elimination of Parvomonas micra and Fusobacterium nucleatum, and a decrease in the quantitative content of Clostridium perfringens and Akkermansia muciniphila. Metagenomic analysis (16S rRNA) revealed an increase in alpha diversity. Conclusion: The introduction of autoprobiotics in the postoperative period is a highly effective and safe approach in the complex treatment of CRC. Future studies will allow the discovery of additional fine mechanisms of autoprobiotic therapy and its impact on the digestive, immune, endocrine, and neural systems.

Introduction: Despite great advances in the treatment of oncological diseases, the development of medical technologies to prevent or reduce complications of therapy, in particular, those associated with surgery and the introduction of antibiotics remains relevant. The aim of the study: to evaluate the effectiveness of the use of autoprobiotics based on indigenous non-pathogenic strains of Enterococcus faecium and Enterococcus hirae in the complex therapy of colorectal cancer (CRC) in the early postoperative period. Materials and methods: A total of 36 patients diagnosed with CRC were enrolled in the study. The study group (Group A) comprised 24 CRC patients who received autoprobiotic therapy in the early postoperative period, while the control group (Group C) included 12 CRC patients without autoprobiotic therapy.Prior to surgery and between days 14-16 post-surgery, comprehensive evaluations were conducted on all patients, encompassing the following: stool and gastroenterological complains analysis. examination of the gut microbiota (bacteriological study, quantitative polymerase chain reaction, metagenome analysis), analysis of interleukins in serum. Results: The use of autoprobiotics led to decrease dyspeptic complaints after surgery, was associated with absence of postoperative complications, did not cause side effects, led to a decrease in the level of pro-inflammatory cytokines (IL-6 and IL-18) in the blood serum. The use of autoprobiotics led to positive changes in the structure of escherichia and enterococci populations, the elimination of Parvomonas micra, Fusobacterium nucleatum, decrease in the quantitative content of Clostridium perfringens, Akkermansia muciniphila. Metagenomic analysis (16S rRNA) revealed an increase in alpha diversity. Conclusion: The administration of autoprobiotics in the postoperative period is highly effective and safe in the comprehensive treatment of colorectal cancer patients. Future studies will allow the discovery of additional fine mechanisms of autoprobiotic therapy and its impact on the digestive, immune, endocrine and neural systems.

The aim of the study was to identify the features of the influence of autoprobiotic and probiotic E. faecium strains on clinical and laboratory parameters in children with functional gastroenterological pathology.Patients and methods. In the period 2020–2021. 35 children over 3 years of age with functional gastrointestinal disorders (FGID) were observed on an outpatient basis, who were prescribed an autoprobiotic or probiotic strain E. faecium for therapeutic purposes and recommended a diet.The study protocol provided for clinical and anamnestic screening, FGID diagnostics; analysis of coprograms and assessment of the composition of the intestinal microbiota in children before and after a course of probiotics. The study of intestinal microbiota was carried out by RT-PCR in feces using a set of primers “Colonoflor”. One patient was excluded from the study due to refusal to take the autoprobiotic.The rest of the patients formed two groups: group 1 (n = 16)—children who received the autoprobiotic strain and group 2 (n = 18)—children who received the conditionally “reference” strain E. faecium L3. Probiotics were used in the form of a liquid form (which is a starter culture based on soy protein isolates containing 10⁹ CFU in 1 ml) at a dose of 25 ml 2 times a day for 10 days.The results of using probiotic strains were evaluated on the basis of data from a survey of parents about the state of children and the dynamics of clinical manifestations, as well as changes in the values of indicators of coprograms and the composition of the intestinal microbiota.Statistical processing of the results was carried out using Student’s t-test, Mann-Whitney U-test; Wilcoxon test. The results were considered reliable at a significance level of p <0.05.Results. Studies of the effect of prescribing E. faecium L3 strain and an autoprobiotic strain to children with FROP revealed a positive effect on the clinical symptoms of the disease (in 29.4% and 25%, respectively) in the absence of undesirable side effects. Analysis of the dynamics of coprograms revealed an equivalent positive dynamics in the form of improvement in most of the studied stool characteristics. The study of the composition of the intestinal microbiota in the observed children revealed a low level of Lactobacilli and Enterococcus before and after the administration of probiotics. After the course of the E. faecium L3 strain, a significant increase in the content of F. prausnitzii was noted. The use of an autoprobiotic strain contributed to a significant decrease in the frequency of isolation of conditionally pathogenic bacteria in large numbersConclusion. The course appointment of autoprobiotic and probiotic E. faecium strains in children with FGID has a positive effect on clinical symptoms, improvement of most coprogram indices and normalization of the intestinal microbiota composition.

Objective. To evaluate the experience of using a probiotic strain of enterococcus in long-term nutritional support of premature infants.Characteristics of children and research methods. The children were selected in 2014–2015. The study included 11 premature babies of the first year of life, treated with antibiotics for a long time. Inclusion criteria: prematurity of any severity, signed informed consent from parents to conduct an outpatient rehabilitation program for children, including long-term nutritional support with a probiotic strain of enterococcus. Exclusion criteria: gross congenital malformations; hereditary metabolic diseases, severe forms of organic pathology of the central nervous system. Exclusion criteria: severe forms of somatic and infectious pathology, use of other probiotics. The probiotic strain of enterococcus – E. faecium L3 (No. RU. 77.99.26.009.Е.002272.02.11) in the liquid form (biomass grown on a dairy-free basis) with a titer of at least 10⁸ CFU/ml was orally administered to children 1 ml 2 times a day while eating. The authors evaluated safety and clinical efficacy of long-term use of the probiotic strain of enterococcus.Results. The authors noted the effectiveness of long-term use of a probiotic strain of enterococcus as nutritional support for premature infants for adequate psychomotor and physical development. They revealed the disappearance of the manifestations of atopic dermatitis while taking a probiotic strain of enterococcus. In the course of the study, the safety of long-term intake of a probiotic strain of enterococcus was established as an integral part of the rehabilitation program for premature babies, who completed their nursing in a hospital.Conclusion. The continuation of studies to assess the clinical efficacy of long-term intake of probiotics in children of the first year of life who were born prematurely and received antibiotics for a long time can be considered very promising.

Although the government has been increasing its investment in sports in public services in recent years, the matching between these investments and people's actual needs for fitness is not high, and people's satisfaction is not high. At present, the national fitness plan for all is still relatively macro. It does not combine the people's fitness needs with their physical health needs, and does not form a systematic sports training index system, nor does it have a clear special plan. The lack of a set of systematic sports training indicators and a set of clear monitoring methods has caused certain constraints on the effective promotion of security measures such as organization construction, human resources construction, site and facilities construction, which has led to the imperfection of China's public service system and the single operation mechanism, and there is a big gap between the growing needs of the people for fitness and health. Therefore, this paper proposes analytic hierarchy process (AHP) to evaluate the automatic monitoring of sports training indicators. The experimental results showed that in sample 1, when the monitoring object was 250, the monitoring accuracy of platform 1 and platform 2 were 56.9% and 78.8%respectively. In sample 2, when the monitoring object was 250, the monitoring accuracy of platform 1 and platform 2 were 64.5% and 90.1% respectively. It can be seen that the monitoring accuracy of platform 2 was higher than that of platform 1 no matter which sample.