Fungi are among the microorganisms able to generate electricity as a result of their metabolic processes. Throughout the last several years, a large number of papers on various microorganisms for current production in microbial fuel cells (MFCs) have been published; however, fungi still lack sufficient evaluation in this regard. In this review, we focus on fungi, paying special attention to their potential applicability to MFCs. Fungi used as anodic or cathodic catalysts, in different reactor configurations, with or without the addition of an exogenous mediator, are described. Contrary to bacteria, in which the mechanism of electron transfer is pretty well known, the mechanism of electron transfer in fungi-based MFCs has not been studied intensively. Thus, here we describe the main findings, which can be used as the starting point for future investigations. We show that fungi have the potential to act as electrogens or cathode catalysts, but MFCs based on bacteria–fungus interactions are especially interesting. The review presents the current state-of-the-art in the field of MFC systems exploiting fungi.

The abundance of cellulosic wastes make them attractive source of energy for producing electricity in microbial fuel cells (MFCs). However, electricity production from cellulose requires obligate anaerobes that can degrade cellulose and transfer electrons to the electrode (exoelectrogens), and thus most previous MFC studies have been conducted using two-chamber systems to avoid oxygen contamination of the anode. Single-chamber, air-cathode MFCs typically produce higher power densities than aqueous catholyte MFCs and avoid energy input for the cathodic reaction. To better understand the bacterial communities that evolve in single-chamber air-cathode MFCs fed cellulose, we examined the changes in the bacterial consortium in an MFC fed cellulose over time. The most predominant bacteria shown to be capable electron generation was Firmicutes, with the fermenters decomposing cellulose Bacteroidetes. The main genera developed after extended operation of the cellulose-fed MFC were cellulolytic strains, fermenters and electrogens that included: Parabacteroides, Proteiniphilum, Catonella and Clostridium. These results demonstrate that different communities evolve in air-cathode MFCs fed cellulose than the previous two-chamber reactors.

Microbial fuel cell (MFC) has the potential to become a promising sustainable technology of wastewater treatment. Usually, the investigations on MFCs are aimed at maximized power production in the system. In this article, we focused on the optimization of wood industry wastewater treatment in MFC, in combination with municipal wastewater as a source of microorganisms. We investigated the influence of different external resistance (2000 Ω, 1000 Ω, 500 Ω, and 100 Ω) on power density and wastewater treatment efficiency (chemical oxygen demand (COD) removal) in 1-month MFC operation time. We found that the highest COD removal was for MFCs under R = 1000 Ω after 22 days of MFC operation, while the highest current density was obtained for the lowest applied resistance. The results imply that wastewater treatment parameters such as resistance and time of MFC operation should be a subject of optimization for each specific type of wastewater used, in order to maximize either wastewater treatment efficiency or power production in MFC. Thus, optimization of power production and COD removal efficiency in MFCs need to be run separately as different resistances are required for maximizing these two parameters. When COD removal efficiency is a subject of optimization, there is no universal value of external resistance, but it should be set to the specific wastewater characteristics.