Point-of-care testing (POCT) plays an increasingly important role in biomedical research and health care. Quantitative phase microscopes (QPMs) with good contrast, no invasion, no labeling, high speed and automation could be effectively applied for POCT. However, most QPMs are fixed on the optical platform with bulky size, lack of timeliness, which remained challenging in POCT solutions. In this paper, we proposed a plug-and-play QPM with multimode imaging based on the quantitative differential phase contrast (qDPC) method. The system employs a programmable LED array as the light source and uses the GPU to accelerate the calculation, which can realize multi-contrast imaging with six modes. Accurate phase measurement and real-time phase imaging are implemented by the proposed qDPC algorithms for quantitative phase targets and biomedical samples. A 3D electric control platform is designed for mechanical control of field of view and focusing without manual operations. The experimental results verify the robustness and high performance of the setup. Even a rookie could finish the POCT scheme for biomedical applications at the scene using the QPM with a compact size of 140 × 165 × 250 mm³.

The hyperspectral image (HSI) distinguishes itself in material identification through its exceptional spectral resolution. However, its spatial resolution is constrained by hardware limitations, prompting the evolution of HSI super-resolution (SR) techniques. Single HSI SR endeavors to reconstruct high-spatial-resolution HSI from low-spatial-resolution inputs, and recent progress in deep learning-based algorithms has significantly advanced the quality of reconstructed images. However, convolutional methods struggle to extract comprehensive spatial and spectral features. Transformer-based models have yet to harness long-range dependencies across both dimensions fully, thus inadequately integrating spatial and spectral data. To solve the above problem, in this paper, we propose a new HSI SR method, SSAformer, which merges the strengths of CNNs and Transformers. It introduces specially designed attention mechanisms for HSI, including spatial and spectral attention modules, and overcomes the previous challenges in extracting and amalgamating spatial and spectral information. Evaluations on benchmark datasets show that SSAformer surpasses contemporary methods in enhancing spatial details and preserving spectral accuracy, underscoring its potential to expand HSI’s utility in various domains, such as environmental monitoring and remote sensing.

Quantitative differential phase contrast (qDPC) imaging has become an important method of optical measurement and life science research in microscopy because of its high reconstruction resolution and non-invasive, high-contrast and quantitative imaging of biological samples. Despite the continuous development of the principle and algorithm, the frame rate of the existing qDPC algorithm is still much lower than that of camera acquisition, so it is hardly applied to real-time image the fast-moving biological samples. In this paper, based on color-coded multiplexing strategy, a compact real-time quantitative phase imaging system is designed to realize multi-mode imaging. The system employs a programmable LED array to illuminate directly, and the phase reconstruction algorithm is deployed in the graphics processing unit (GPU) of the laptop to accelerate the calculation. The system can achieve high-speed quantitative phase imaging of non-stained biological samples, and the frame rate can reach 60fps. The device has the advantages of compact structure, low cost and portability. Thus, it is suitable for mobile medical applications.