Fusarium root rot, caused by Fusarium spp. in alfalfa (Medicago sativa L.), adversely impacts alfalfa by diminishing plant quality and yield, resulting in substantial losses within the industry. The most effective strategy for controlling alfalfa Fusarium root rot is planting disease-resistant varieties. Therefore, gaining a comprehensive understanding of the mechanisms underlying alfalfa’s resistance to Fusarium root rot is imperative. In this study, we observed the infection process on alfalfa seedling roots infected by Fusarium acuminatum strain HM29-05, which is labeled with green fluorescent protein (GFP). Two alfalfa varieties, namely, the resistant ‘Kangsai’ and the susceptible ‘Zhongmu No. 1’, were examined to assess various physiological and biochemical activities at 0, 2, and 3 days post inoculation (dpi). Transcriptome sequencing of the inoculated resistant and susceptible alfalfa varieties were conducted, and the potential functions and signaling pathways of differentially expressed genes (DEGs) were analyzed through gene ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Meanwhile, a DEG co-expression network was constructed though the weighted gene correlation network analysis (WGCNA) algorithm. Our results revealed significant alterations in soluble sugar, soluble protein, and malondialdehyde (MDA) contents in both the ‘Kangsai’ and ‘Zhongmu No. 1’ varieties following the inoculation of F. acuminatum. WGCNA analysis showed the involvement of various enzyme and transcription factor families related to plant growth and disease resistance, including cytochrome P450, MYB, ERF, NAC, and bZIP. These findings not only provided valuable data for further verification of gene functions but also served as a reference for the deeper explorations between plants and pathogens.

Oedaleus decorus asiaticus (O. decorus) is a significant pest in the grasslands of Inner Mongolia, posing considerable challenges to the development of animal husbandry. To understand the key factors influencing the population distribution of O. decorus, field surveys were conducted from 2018 to 2020, during which the population count, growth stage, and location information of O. decorus were recorded. Daily soil moisture (SM) data and daily land surface temperature (LST) data were obtained from the National Tibetan Plateau Data Center, and a Generalized Additive Model (GAM) was constructed. Our findings indicate that the SM (S8) in August of the previous year is the most critical factor, with an F-value of 27.422, followed by the LST (L10) in October of the previous year, the LST (L6) in June of the survey year, the SM (S9) in September of the previous year, the LST (L3) in March of the survey year, and the LST (L5) in May of the survey year, with F-values of 7.848, 7.223, 5.823, 4.919, and 3.547, respectively. S8 and S9 can be regarded as vital indicators for predicting and monitoring the occurrence of O. decorus. However, the contributions of S8 and S9 to O. decorus density differ considerably. S8 is negatively correlated with O. decorus density, while S9 values below 0.29 m³/m³ can promote the growth of O. decorus. A higher LST during early overwintering correlates with increased O. decorus density. During the survey year, LST emerged as the primary factor affecting grasshopper density. Additionally, it plays a more complex role during incubation periods. This study clearly identifies SM and LST as the major factors influencing the occurrence of O. decorus, which will aid in predicting and monitoring its density.