Background. The search for ways to use logging waste is a distinct scientific direction, due to the large number of their permanent formation. The importance of such studies is associated with the need to reduce the loss of valuable wood raw materials in the form of logging waste. One of the possible ways of using waste from logging industries is the production of wood chips using a mobile complex of machines with its further recycling into a wood-fiber semi-finished product. Wood-fiber semi-finished product is, as it is known nowadays, one of the environmentally friendly types of insulation and finishing materials. A significant limitation in the use of wood fiber in various types of production is low fire and bio-resistance. As a consequence, imparting specific properties to a wood-fiber semi-finished product in the form of fire and bio-resistance is an acute problem of the modern wood processing industry. Purpose. Synthesis of 4-azofunctionalized pyrazoles with perfluoroalkyl substituent by cyclocondensation of polyfluorinated beta-diketones to confer special properties to the wood-fiber semi-finished product. Materials and methods. The 1H, 13C NMR and 1H-13C HSQC spectra were registered at the Krasnoyarsk Regional Center for Collective Use of the FITC KNC SB RAS on a Bruker Avance III Fourier spectrometer with a frequency of 600 MHz equipped with sensitive helium probes with a diameter of 5 mm and 1.7 mm. TMS is used as an internal standard. Chromato-mass spectra were obtained using ISQ 7610 Single Quadrupole GC-MS and Shimadzu LC/MS-2020 instruments. Methyl alcohol was used as the mobile phase. IR spectra were recorded on the FT-801 (FT-801) SIMEX IR Fourier spectrometer with a spectral range from 450 to 5700 cm-1. UV spectra were recorded on a HELIOS OMEGA spectrophotometer equipped with a tungsten-halogen lamp in quartz cuvettes with a diameter of 1 cm at a concentration of 1·10-4 mol/l for 200-400 nm and a concentration of 1·10-2 mol/l for 400-800 nm in ethanol. Melting temperatures were determined in open capillaries on a PTP device (TU 25-11-1144-76). Results. 3 new compounds were synthesized: 3(5)-(thiophene-2-yl)-4-nitroso-5(3)-(trifluoromethyl)-1H-pyrazole, 3(5)-phenyl-4-nitroso-5(3)-(trifluoromethyl)-1H-pyrazole and 3(5)-(naphthalene-2-yl)-4-nitroso-5(3)-(trifluoromethyl)-1H-pyrazole. Conclusion. Thus, 3(5)-(thiophene-2-yl)-4-nitroso-5(3)-(trifluoromethyl)-1H-pyrazole, 3(5)-phenyl-4-nitroso-5(3)-(trifluoromethyl)-1H-pyrazole and 3(5)-(naphthalene-2-yl)-4-nitroso-5(3)-(trifluoromethyl)-1H-pyrazole. Complex separable mixtures of substances containing pyrazoline, β-diketone and target nitrosopyrazoles were formed by a single-reactor method. The two-stage method of production proposed by us increases the purity and yield of the target products.

A number of hitherto unknown trifluorinated beta-diketone oxymes were obtained by direct nitrosation with sodium nitrite in an acidic medium. 4,4,4-Trifluoro-2-(hydroxyimino)-1-(thiophene-2-yl)butane-1,3-dione and 4,4,4-trifluoro-2-(hydroxyimino)-1-phenylbutane-1,3-dione have been characterized by various physicochemical methods. The structure of the formed compounds was confirmed using FTIR, UV-visible spectroscopy, NMR and GC-MS. The spectral characteristics of the obtained compounds were modeled using DFT and TD-DFT calculations.

Bovine serum albumin nanoparticles (BSA-NPs) are widely used as model protein nanocarriers, and their physicochemical properties are strongly influenced by synthesis conditions. This study evaluated the effect of four desolvating agents (DAs), namely acetone, ethanol, isopropanol, and acetonitrile, differing in polarity and hydrogen-bonding capacity, combined with glutaraldehyde (GA) concentration, stirring speed, and DA addition rate, across 32 formulations characterized by dynamic light scattering (DLS) and scanning transmission electron microscopy (STEM). Ethanol-derived BSA-NPs were most reproducible, with hydrodynamic diameter (D_h) of 99.2 to 124.8 nm, polydispersity index (PDI) of 0.076 to 0.193, and ζ-potential of −20.78 to −23.06 mV, independent of other parameters. Acetone-derived particles were smaller but more sensitive to stirring speed, with PDI up to 0.642. Acetonitrile-derived particles showed pronounced aggregation, with PDI never below 0.404, while isopropanol-derived particles showed the strongest glutaraldehyde-dependent aggregation, with PDI up to 0.964, despite a primary size similar to ethanol. DA identity was the dominant factor governing NP properties, and its selection should precede optimization of the other parameters. DA identity further affected both resistance to trypsin digestion and drug-loading capacity. Particles produced with acetonitrile were the most resistant to proteolysis. In addition, hydrophilic Rhodamine B was better loaded into ethanol-derived carriers, and hydrophobic Nile Red was preferentially loaded into particles made via acetone desolvation.

Unique physicochemical properties of ferritin nanoparticles, such as high biocompatibility, precise structural assembly, and modular surface chemistry make them a promising biomimetic platform for the delivery of therapeutic and diagnostic agents, including radioactive isotopes. However, the translation of ferritin from laboratory-scale synthesis to clinical applications remains hindered by the lack of standardized analytical workflows and requires precise qualitative and quantitative analysis of core loading, structural integrity, and long-term stability. This review systematically examines current characterization techniques ranging from morphological evaluation and quantitative elemental analysis to advanced spectroscopic and functional performance assays for evaluating metal and non-metal encapsulation efficiency, release kinetics, stability in complex biological media, and functional performance of ferritin-based nanocarriers. We highlight the correlation between the analytical metrics and biomedical outcomes, addressing the critical challenges in standardization necessary for clinical translation. The discussed methodologies establish a methodological roadmap for the rational design and quality control of ferritin-based theranostic systems, facilitating their path toward clinical implementation.

Sepsis remains a major global health challenge due to its complex pathophysiology and limited therapeutic options. Nanomedicine offers innovative strategies to address these limitations by enabling diverse nanoparticle designs and mechanisms that modulate the septic response. This review examines the dynamic interactions between nanoparticles and the immune system, with a focus on how protein corona formation shapes nanoparticle behavior, biodistribution, and therapeutic efficacy. Disease-specific protein corona profiles can serve as pathology “fingerprints” for diagnosis and targeted delivery, and their controlled formation is now emerging as a therapeutic tool rather than only a diagnostic readout. While the protein corona is a spontaneous biomolecular layer, its composition can be rationally steered to support defined therapeutic goals. In this context, decoy nanoparticles are engineered to sequester pathogens or inflammatory mediators, such as cytokines, histones, and neutrophil extracellular traps, thereby mitigating inflammation and tissue damage. This review discusses how protein corona engineering can potentiate decoy strategies in sepsis diagnosis and therapy, highlighting key platforms including macrophage‑like nanoparticles that neutralize endotoxins and cytokines, histone‑binding hydrogels, and mesoporous silica nanoparticles that scavenge cell‑free DNA and inhibit Toll‑like receptor activation. We also address how Artificial Intelligence can improve prediction of protein corona dynamics and identification of disease‑specific protein signatures, enabling more personalized nanodecoy design. Given the highly dynamic and heterogeneous nature of sepsis, characterized by evolving circulating mediators and protein profiles, integrating protein corona control with decoy mechanisms offers a multifaceted route to limit immune dysregulation, enhance drug delivery, and reduce organ damage, paving the way toward precision nanomedicine in sepsis.