Site specific control of the crystallographic orientation of grains within metal components has been unachievable before the advent of metals additive manufacturing (AM) technologies. To demonstrate the capability, the growth of highly misoriented micron scale grains outlining the letters D, O and E, through the thickness of a 25·4 mm tall bulk block comprised of primarily columnar [001] oriented grains made of the nickel base superalloy Inconel 718 was promoted. To accomplish this, electron beam scan strategies were developed based on principles of columnar to equiaxed transitions during solidification. Through changes in scan strategy, the electron beam heat source can rapidly change between point and line heat source modes to promote steady state and/or transient thermal gradients and liquid/solid interface velocity. With this approach, an equiaxed solidification in the regions bounding the letters D, O and E was achieved. The through thickness existence of the equiaxed grain structure outlining the letters within a highly columnar [001] oriented bulk was confirmed through characterizing the bulk specimen with energy selective neutron radiography and confirming with an electron backscatter detection. Ultimately, this demonstration promotes the ability to build metal components with site specific control on crystallographic orientation of grains using the electron beam melting process.

Two optical configurations are commonly used in single-molecule fluorescence microscopy: point-like excitation and detection to study freely diffusing molecules, and wide field illumination and detection to study surface immobilized or slowly diffusing molecules. Both approaches have common features, but also differ in significant aspects. In particular, they use different detectors, which share some requirements but also have major technical differences. Currently, two types of detectors best fulfil the needs of each approach: single-photon-counting avalanche diodes (SPADs) for point-like detection, and electron-multiplying charge-coupled devices (EMCCDs) for wide field detection. However, there is room for improvements in both cases. The first configuration suffers from low throughput owing to the analysis of data from a single location. The second, on the other hand, is limited to relatively low frame rates and loses the benefit of single-photon-counting approaches. During the past few years, new developments in point-like and wide field detectors have started addressing some of these issues. Here, we describe our recent progresses towards increasing the throughput of single-molecule fluorescence spectroscopy in solution using parallel arrays of SPADs. We also discuss our development of large area photon-counting cameras achieving subnanosecond resolution for fluorescence lifetime imaging applications at the single-molecule level.

The unique capability of microchannel plates (MCPs) to convert a single photon/ electron/ ion/ neutron into a charge of 10⁴ -10⁷ electrons localized within 4-12 μm from the event position is widely used in event counting imaging detectors. The high spatial and timing resolution of MCP detectors have been demonstrated with different readout techniques. A compromise between the spatial and temporal resolution, the global/local counting rate and active area must always be made for each detector application. In this paper we present a 28 × 28 mm² MCP detector with 2 × 2 Timepix ASICs for readout, capable of ~ 10 μm spatial resolution at event rates up to ~ 3 MHz, and in excess of 200 MHz with ~ 55 μm pixels. This detector has a unique capability to detect multiple simultaneous events, up to several thousand with ~ 10 μm resolution and > 25000 for the 55 μm mode. The latter is enabled by the new fast readout electronics capable of readout speeds of ~ 1200 frames/sec. Despite its limitations (relatively small active area, readout dead time of 300 μs) the MCP-Timepix detector can be very attractive for applications where high spatial resolution needs to be preserved for nearly simultaneous events, e.g., time of flight measurements with pulsed sources. The low noise of the Timepix readout enables operation at gains as low as 10⁴ -10⁵, which should extend the lifetime of the MCP detectors operating at high counting rates.