Antibiotic resistance is a growing public health challenge. Antimicrobial peptides (AMPs) effectively target microorganisms through non-specific mechanisms, limiting their ability to develop resistance. Therefore, the prediction and design of new AMPs is crucial. Recently, deep learning has spurred interest in computational approaches to peptide drug discovery. This study presents a novel deep learning framework for AMP classification, function prediction, and generation. We developed discoverAMP (dsAMP), a robust AMP predictor using CNN Attention BiLSTM and transfer learning, which outperforms existing classifiers. In addition, dsAMPGAN, a Generative Adversarial Network (GAN)-based model, generates new AMP candidates. Our results demonstrate the superior performance of dsAMP in terms of sensitivity, specificity, Matthew correlation coefficient, accuracy, precision, F1 score, and area under the ROC curve, achieving >95% classification accuracy with transfer learning on a small dataset. Furthermore, dsAMPGAN successfully synthesizes AMPs similar to natural ones, as confirmed by comparisons of physical and chemical properties. This model serves as a reliable tool for the identification of novel AMPs in clinical settings and supports the development of AMPs to effectively combat antibiotic resistance.

In response to the demand for the evaluation of the actual navigation performance (ANP) of rotorcraft logistics uncrewed aerial vehicle (UAV) navigation systems in urban scenarios, this paper proposes a method for evaluating the ANP of rotorcraft logistics UAVs based on the Improved Sparrow Search Algorithm (ISSA). Taking ANP as the optimization objective, an optimization model for the ANP of rotorcraft logistics UAVs is constructed. Based on the probability of the UAV’s actual position falling within the error circle, an initial population strategy based on probabilistic decision-making is designed, and an adaptive dynamic step size strategy and dynamic compression search strategy are proposed to improve the traditional Sparrow Search Algorithm (SSA), enhancing the algorithm’s ability of optimization and to escape local extremum. The contribution of this paper mainly includes constructing the ANP optimization model and designing the ISSA method. Experimental results show that the proposed method can effectively estimate ANP, achieve onboard performance monitoring and warning, and ensure the required navigation performance (RNP) and flight safety of UAVs.

This work presents a minimum separation calculation for the integrated operation of manned and unmanned aerial vehicles in an uncertain airspace environment. Different from traditional path-planning-based research, this study investigated the minimum safe separation distance from a novel perspective of reachability analysis. The proposed computational method made use of the Hamilton–Jacobi partial differential equation (HJPDE) to obtain the backward reachable tube. Firstly, this work modeled the integrated operation in the UAS traffic management scenario, particularly focusing on the uncertainties. Then, a probabilistic reachability tube computation method was derived. Next, this work calculated the safe separation distances based on reachability analysis for three scenarios: a deterministic environment, an environment with relative position uncertainty, and an environment with relative heading angle uncertainty. By calculating the reachable tubes for a given response time, the worst-case minimum safe distances from the UAV’s perspective were determined, and the quantitative patterns were summarized. The results in this work indicate that, with an increase in the risk level and under the premise of a 1 s response time, the minimum safe separation increases from 26.7 m to 30.0 m. Finally, the paper discusses the results, explaining their rationality from both mathematical and physical perspectives.