The restriction on the use of antibiotics in poultry has led to an increase in the use of natural products that could serve as alternatives to antibiotics. Mushrooms contain bioactive compounds that exhibit antifungal, antiparasitic, antibacterial, antioxidant, antiviral, anti-inflammatory, and cytotoxic properties. Hence, they are being tested, revealing as performance-enhancing natural feed additives for livestock. This review focused on the role of different species of mushrooms commonly used in poultry on the performance, immunomodulatory actions, cholesterolemic properties, and meat quality of poultry birds. Different studies reviewed show that mushrooms could positively impact poultry production, improve growth performance, modulate immune response, exert tissue antioxidant activity, influence intestinal morphology, enhance gut microbiome, and improve lipid profile. The variations in their efficacy could be attributed to the variations in physicochemical properties of different species and dosage levels applied in the experiments. However, the use of mushrooms as a natural product supplement is in its infancy, and more basic, pilot and large-scale research is required to make it a viable approach for improving immune responses in the poultry industry.

This study evaluated the effects of varying levels of dietary green tea extract (GTE) on growth performance, Clostridium perfringens (CP) colonization, and inflammatory responses in broiler chickens during experimental subclinical necrotic enteritis (SNE). In a 21-day experiment, 480 one-day-old male broiler (Ross 708) chicks were equally allotted into four dietary treatment groups. From days 1 to 10, all groups received a corn–soy basal diet, switching to a wheat-fish diet on day 11. Treatments included CON (no GTE), GTX, GTY, and GTZ (250, 500, and 1000 mg/kg GTE, respectively). On day 11, each group split into ACON, AGTX, AGTY, and AGTZ, totaling eight treatments. From days 17 to 20, ACON, AGTX, AGTY, and AGTZ chicks were orally challenged twice daily with 3 mL CP26 (2.5 × 10⁸ CFU/mL). On d 16 (pre-challenge) and d 1 post-challenge, ileo-jejunal contents were collected for CP enumeration, and jejunum tissue was analyzed via qRT-PCR to determine the expression levels of IL-1β, IFNγ, TNF-α, TGFβ, IL-10, and MUC2 genes. Challenged chicks showed poorer (p ≤ 0.05) growth and higher intestinal CP, with a potential improvement in GTE-supplemented diets. Findings suggests that dietary GTE supplementation mitigated the characteristic growth depression during SNE, reduced intestinal CP infection, and modulated inflammatory response in broiler chicks.

Dietary polyunsaturated fatty acids (PUFAs) are vital for brain health and cognitive function. The dorsal raphe nucleus (DRN) regulates mood via serotonin, while the hypothalamus (HYP) controls energy homeostasis. Flaxseed oil (FLAX) is rich in omega-3 PUFAs like α-linolenic acid (ALA), and has been reported to influence serotonergic signaling in mammals, but data in poultry are scarce. This study investigated the effects of FLAX on metabolites crossing the blood-brain barrier (BBB) to serotonergic brain regions and on growth performance in broiler chickens. Day-old chicks (n = 160) were assigned to two diets (5 replicates/treatment): control (CON; poultry fat-based diet) or FLAX (3% inclusion level). Growth performance was recorded, and DRN, HYP, and plasma were analyzed using HPLC-MS metabolomics. Serotonin and its metabolite 5-HIAA were quantified using LC-MS/MS. FLAX-fed birds had higher body weight gain (p < 0.0055) and better feed conversion ratio (p < 0.0049) than CON. Metabolomics identified 2271 features, of which 650 were annotated as metabolites. Of 35 differentially abundant plasma metabolites, eight were also differentially abundant in brain tissues. In the DRN, tryptophan (serotonin precursor) and corydaline (neuroprotective) were upregulated. Serotonin levels were significantly higher in both the DRN and HYP of FLAX-fed birds compared to CON. This suggest that dietary flaxseed oil may modulate stress responses, behavior, and welfare in broilers. In the HYP, dethiobiotin (energy), galanthamine (neuroprotective), and gambogic acid (antioxidative) were upregulated, while xanthoxyletin (anti-inflammatory) was downregulated. In conclusion, flaxseed oil improved growth and elevated serotonin in the DRN and HYP via enhanced tryptophan availability, suggesting potential benefits for stress resilience and welfare.

Onion peel extract (OPE) is rich in polyphenolic compounds with antimicrobial potential. Salmonella Enteritidis (SE) infection in young broiler chicks causes morbidity, reduced growth, and contributes to human gastroenteritis through contaminated poultry products. The spleen is a key secondary lymphoid organ coordinating systemic responses to pathogens in chicken. This study evaluated how dietary OPE influences spleen metabolic profiles during SE infection. Day-old Ross 708 male chicks (n = 128) were assigned to four treatments: CON, CON-SE, OPE (6 g/kg), and OPE-SE. Chicks in CON and OPE received sterile broth, whereas CON-SE and OPE-SE received 2.25 × 10⁸ CFU/mL SE at 2 d of age. At 5 and 12 dpi, spleens from six chicks per treatment were collected for untargeted HPLC-MS metabolomics. A total of 857 metabolites were identified and analyzed using MetaboAnalyst 6.0 (p < 0.05; fold change ≥ 2.0; VIP score > 1.0). In CON-SE chicks, energy generating metabolites (6-phosphogluconic acid, methylmalonic acid, propionic acid) increased, while 13,14-dihydro-15-keto-prostaglandin D2 and kynurenic acid decreased. Dietary OPE elevated several dipeptides (L-Val-Gly, L-Leu-Gly, Gly-Gly-Leu, L-Val-L-Met) and reduced ATP linked metabolites (3,6-di-O-methyl-beta-D-glucose and 3-O-beta-D-galactosyl-sn-glycerol). Enrichment analysis showed that SE infection altered valine, leucine, and isoleucine degradation and aromatic amino acid biosynthesis, whereas OPE enriched galactose and biotin metabolism in uninfected chicks, but enriched tryptophan, taurine and hypotaurine metabolism in SE-infected chicks. Overall, dietary OPE optimized response of metabolic pathways associated with immune activation, unlike corresponding pathways in CON-SE birds.

Background Undernutrition immensely contribute to the death of almost half of the children under 5 years, translating into the loss of about 3 million young lives a year. In developing countries, penultimate to hunger that constitute to the death of most children, Protein Energy Malnutrition (PEM) amongst other factors account for the death of 49% of children below the age of five. Due to incessant increase in poverty level in Uganda, Jinja District five-year development plan of 2011 was designed to curb menace posed by malnutrition not only on children but also adults in this district. The objective of this study is to assess the prevalence and factors associated with PEM among under-fives admitted in the paediatric wards of Jinja Regional Referral Hospital (JRRH). Methods To carry out this study, a descriptive questionnaire-based and facility-based cross-sectional study design was used which will apply both qualitative and quantitative approaches. The study involved a sample size of 364 with the study population being under-fives admitted in the paediatric wards of JRRH between March and August 2019.Results Results of this study shows that 52 out of 364 admissions were due to PEM giving a prevalence of 14.29%, males being affected more than females, with suboptimal immunization history. Children aged between 12 – 24 months contributed the highest (34.62%) cases of PEM and most are living in rural areas with low family socio-economic status and poor breastfeeding and feeding practices. The under-five PEM case-fatality rate was 2% (1 out of 52). Conclusion In conclusion, both the prevalence and case fatality rates of under-five PEM were high, calling for appropriate interventions based on the associated factors.