Graphene oxide nanoparticles (GONPs) have attracted a lot of attention due to their many applications. These applications include batteries, super capacitors, drug delivery and biosensing. However, few studies have investigated the effects of these nanoparticles on the immune system. In this study, the in vitro effects of GONPs on the immune system was evaluated by exposing murine macrophages, RAW 264.7 cells and human whole blood cell cultures (to GONPs. The effects of GONPs on RAW cells were monitored under basal conditions. The whole blood cell cultures were exposed to GONPs in the presence or absence of the mitogens lipopolysaccharide (LPS) and phytohaemmagglutinin (PHA). A number of parameters were monitored for both RAW and whole blood cell cultures, these included cytotoxicity, inflammatory biomarkers, cytokines of the acquired immune system and a proteome profile analysis. The GONPs were cytotoxic to both RAW and whole blood cell cultures at 500 μg/mL. In the absence of LPS, GONPs elicited an inflammatory response from the murine macrophage, RAW and whole blood cell cultures at 15.6 and 5 μg/mL respectively. This activation was further corroborated by proteome profile analysis of both experimental cultures. GONPs inhibited LPS induced interleukin 6 (IL-6) synthesis and PHA induced interferon gamma (IFNγ) synthesis by whole blood cell cultures in a dose dependent manner. In the absence of mitogens, GONPs stimulated IL-10 synthesis by whole blood cell cultures. The current study shows that GONPs modulate immune system biomarkers and that these may pose a health risk to individuals exposed to this type of nanoparticle.

Carbon dots (CDs) are engineered nanoparticles that are used in a number of bioapplications such as bioimaging, drug delivery and theranostics. The effects of CDs on the immune system have not been evaluated. The effects of CDs on the immune system were assessed by using RAW 264.7 cells and whole blood cell cultures. RAW cells were exposed to CD concentrations under basal conditions. Whole blood cell cultures were exposed to CD concentrations under basal conditions or in the presence of the mitogens, lipopolysaccharide (LPS) or phytohaemmagglutinin (PHA). After exposure, a number of parameters were assessed, such as cell viability, biomarkers of inflammation, cytokine biomarkers of the acquired immune system and a proteome profile analysis. CDs were cytotoxic to RAW and whole blood cell cultures at 62.5, 250 and 500 μg/mL, respectively. Biomarkers associated with inflammation were induced by CD concentrations ≥250 and 500 μg/mL under basal conditions for both RAW and whole blood cell cultures, respectively. The humoral immune cytokine interleukin (IL)-10 was increased at 500 μg/mL CD under both basal and PHA activated whole blood cell culture conditions. Proteome analysis supported the inflammatory data as upregulated proteins identified are associated with inflammation. The upregulated proteins provide potential biomarkers of risk that can be assessed upon CD exposure.

Phenolic compounds are the main phytochemical constituents of many higher plants. They play an important role in synthesizing metal nanoparticles using green technology due to their ability to reduce metal salts and stabilize them through physical interaction/conjugation to the metal surface. Six pure phenolic compounds were isolated from licorice (Glycyrrhiza glabra) and employed in synthesizing gold nanoparticles (AuNPs). The isolated compounds were identified as liquiritin (1), isoliquiritin (2), neoisoliquiritin (3), isoliquiritin apioside (4), liquiritin apioside (5), and glabridin (6). The synthesized AuNPs were characterized using UV, zeta sizer, HRTEM, and IR and tested for their stability in different biological media. The phenolic isolates and their corresponding synthesized NP conjugates were tested for their potential in vitro cytotoxicity. The anti-inflammatory effects were investigated in both normal and inflammation-induced settings, where inflammatory biomarkers were stimulated using lipopolysaccharides (LPSs) in the RAW 264.7 macrophage cell line. LPS, functioning as a mitogen, promotes cell growth by reducing apoptosis, potentially contributing to observed outcomes. Results indicated that all six pure phenolic isolates inhibited cell proliferation. The AuNP conjugates of all the phenolic isolates, except liquiritin apioside (5), inhibited cell viability. LPS initiates inflammatory markers by binding to cell receptors and setting off a cascade of events leading to inflammation. All the pure phenolic isolates, except isoliquiritin, neoisoliquiritin, and isoliquiritin apioside inhibited the inflammatory activity of RAW cells in vitro.

Titanium dioxide nanoparticles (TiO₂NPs) are found in a wide range of products such as sunscreen, paints, toothpaste and cosmetics due to their white pigment and high refractive index. These wide-ranging applications could result in direct or indirect exposure of these NPs to humans and the environment. Accordingly, conflicting levels of toxicity has been associated with these NPs. Therefore, the risk associated with these reports and for TiO₂NPs produced using varying methodologies should be measured. This study aimed to investigate the effects of various media on TiO₂NP properties (hydrodynamic size and zeta potential) and the effects of TiO₂NP exposure on human colorectal adenocarcinoma (Caco-2) epithelial cell viability, inflammatory and cell stress biomarkers and angiogenesis proteome profiles. The NPs increased in size over time in the various media, while zeta potentials were stable. TiO₂NPs also induced cell stress biomarkers, which could be attributed to the NPs not being cytotoxic. Consequently, TiO₂NP exposure had no effects on the level of inflammatory biomarkers produced by Caco-2. TiO₂NPs expressed some anti-angiogenic properties when exposed to the no-observed-adverse-effect level and requires further in-depth investigation.

The increased production and utilization of nanoparticles (NPs) in consumer products have raised concerns about their potential impact on the environment at national and international levels as they move through different trophic levels. Previous research has shown that they possess the ability to infiltrate cellular and subcellular structures, potentially interfering with important physiological processes and leading to toxicity. Studies have also indicated that nanoparticle toxicity varies significantly with changes in physicochemical properties, even among nanoparticles with identical chemical compositions. Because of this variable toxicity potential, it has become imperative to study the toxicity of these materials on vital physiological systems on a case-by-case basis, particularly before their widespread utilization in consumer products. This study evaluated the cytotoxic effects of selected nanoparticles (i.e. AgNPs, TiO₂NPs, CD NPs, CD-Amine), with a particular emphasis on their toxicity to macrophages. The study involved exposing the immune-representative RAW 264.7 cell line to various concentrations of NPs, both with and without the presence of lipopolysaccharide (LPS). Results showed that silver nanoparticles (AgNPs) and titanium dioxide nanoparticles (TiO₂NPs) had an adverse impact on cell viability under both conditions. The unmodified carbon dot nanoparticles only had a moderate impact on viability. However, toxicity increased significantly when carbon dot NPs were modified with amine groups, surpassing that of metal-based NPs (i.e., AgNPs and TiO₂NPs), highlighting the critical role of surface charge in influencing cytotoxicity. Concurrently, the study also comprehensively assessed the potential neurotoxicity of these nanoparticles by measuring their modulatory potential on acetylcholinesterase (AChE) activity using Ellman's reagent. Findings indicated that both AgNPs and amine-modified carbon dots (CD-Amine) significantly inhibited AChE activity, while TiO₂NPs and CDNPs had no impact on AChE activity. Interestingly, this inhibition was not dependent on whether the nanoparticles were metal- or carbon-based, or the size of the nanoparticles, suggesting that the interaction between nanoparticles and enzymes is likely influenced by the chemistry of the enzyme and the nanoparticles themselves. This study seeks to contribute valuable insights into the diverse biological interactions of nanoparticles, informing risk assessments and the development of safer nanomaterials for various applications.