Robert M Blumenthal

Dr. Robert M Blumenthal

orcid

Research Focus & Metrics

Academic Career Length

51Years (1976-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 June 2003 in Trends in Biochemical Sciences
Verified
Journal Article
Closed access
HSHeidi L Schubert
Heidi L Schubert
RBRobert M Blumenthal
Robert M Blumenthal
Xiaodong ChengXiaodong Cheng
Xiaodong Cheng

1 November 1995 in Journal of Molecular Biology
Verified
Journal Article
Closed access
TMThomas Malone
Thomas Malone
RBRobert M Blumenthal
Robert M Blumenthal
XCXiaodong Cheng
Xiaodong Cheng

1 March 2008 in Structure
Verified
Journal Article
Closed access
Xiaodong ChengXiaodong Cheng
Xiaodong Cheng
RBRobert M Blumenthal
Robert M Blumenthal

1 January 1988 in Nucleic Acids Research
Verified
Journal Article
Closed access
ERE.A. Raleigh
E.A. Raleigh
NMN.E. Murray
N.E. Murray
HRH. Revel
H. Revel
RBRobert M Blumenthal
Robert M Blumenthal
DWD. Westaway
D. Westaway
DHD. Hanahan
D. Hanahan
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected to be sensitive, and this could interfere with cloning experiments. The McrA and B phenotypes of a few strains have been reported previously (1-4). The Mcr phenotypes of 94 strains, primarily derived from E. coli K12, are tabulated here. We briefly review some evidence suggesting that McrB restriction of mouse-modified DNA does occur in vivo and does in fact interfere with cloning of specific mouse sequences.

8 October 2002 in Proceedings of the National Academy of Sciences
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Journal Article
Closed access
TTTravis H. Tani
Travis H. Tani
AKArkady Khodursky
Arkady Khodursky
RBRobert M Blumenthal
Robert M Blumenthal
PBPatrick O. Brown
Patrick O. Brown
RMRowena G. Matthews
Rowena G. Matthews
Bacterial adaptation to nutrient limitation and increased population densities is central to survival and virulence. Surprisingly, 98% of E. coli genes to more comprehensively identify those controlled by Lrp. The primary analysis compared isogenic Lrp + and Lrp − strains in cells growing in steady state in glucose minimal medium, either in the presence or absence of leucine. More than 400 genes were significantly Lrp-responsive under the conditions used. Transcription of 147 genes was lower in Lrp + than in Lrp − cells whether or not leucine was present; most of these genes were tightly coregulated under several conditions, including a burst of synthesis on transition to stationary phase. This cluster includes 56 of 115 genes already known to play roles in stationary phase. Our results suggest that the actual number of genes induced on entrance into stationary phase is closer to 200 and that Lrp affects nearly three-quarters of them, including genes involved in response to nutrient limitation, high concentrations of organic acids, and osmotic stress.

1 October 2002 in Journal of Molecular Evolution
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Journal Article
Closed access
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JBJanusz Bujnicki
Janusz Bujnicki
MFMarcin Feder
Marcin Feder
MRMonika Radlinska
Monika Radlinska
RBRobert M Blumenthal
Robert M Blumenthal