Although phytohormones are known to be important signal molecules involved in wood formation, their roles are still largely unclear. Here, Populus simonii × P. nigra seedlings were treated with different concentrations of exogenous phytohormones, indole-3-acetic acid (IAA), gibberellin (GA₃), and brassinosteroid (BR), and the effects of phytohormones on growth were investigated. Next, 27 genes with known roles in wood formation were selected for qPCR analysis to determine tissue-specificity and timing of responses to phytohormone treatments. Compared to the control, most IAA, GA₃, and BR concentrations significantly increased seedling height. Meanwhile, IAA induced significant seedling stem diameter and cellulose content increases that peaked at 3 and 30 mg·L⁻¹, respectively. Significant increase in cellulose content was also observed in seedlings treated with 100 mg·L⁻¹ GA₃. Neither stem diameter nor cellulose content of seedlings were affected by BR treatment significantly, although slight effects were observed. Anatomical measurements demonstrated improved xylem, but not phloem, development in IAA- and BR-treated seedlings. Most gene expression patterns induced by IAA, GA₃, and BR differed among tissues. Many IAA response genes were also regulated by GA₃, while BR-induced transcription was weaker and slower in Populus than for IAA and GA₃. These results reveal the roles played by phytohormones in plant growth and lay the foundation for exploring molecular regulatory mechanisms of wood formation in Populus.

Flowering regulation in hemp is critical for determining fiber yield, seed production, and the accumulation of medicinal components. This paper, based on bibliometric analysis, highlights the current gap in basic research on cannabis floral organs. The latest advancements in flowering regulation are then systematically reviewed. The morphological and physiological foundations of flowering are examined, including the flowering phenotype, timing, and flower differentiation. Furthermore, the direct regulatory mechanisms of key environmental and cultivation factors—such as photoperiod (type, light quality, duration) and plant nutrition (fertilization, hormones)—on flowering are discussed. Potential pathways through which biotic and abiotic stresses indirectly affect flowering by disrupting metabolic processes are also explored. In addition, the genetic basis of flowering regulation, including key gene loci such as Autoflower1, Early1, and CsPRR37, as well as molecular networks like the FT-mediated photoperiod pathway and the miR156-SPL age pathway, is examined in detail. Finally, the industrial significance of flowering regulation is summarized, and future research directions are proposed to provide a theoretical foundation for the precise breeding and cultivation management of high-quality hemp varieties.

This study aims to elucidate the molecular mechanisms underlying cyanogenic glycoside accumulation in flax. As an important oil and fiber crop, the nutritional value of flax is compromised by the toxicity of cyanogenic glycoside. To clarify the key genetic regulators and temporal patterns of cyanogenic glycoside biosynthesis, transcriptomic sequencing was performed on seeds from high- and low-cyanogenic glycoside flax varieties (‘MONTANA16’ and ‘Xilibai’) at three developmental stages: bud stage, full flowering stage, and capsule-setting stage. A total of 127.25 Gb of high-quality data was obtained, with an alignment rate exceeding 87.80%. We identified 31,623 differentially expressed genes (DEGs), which exhibited distinct variety- and stage-specific expression patterns. Principal component analysis (PCA) and hierarchical clustering demonstrated strong reproducibility among biological replicates and revealed the seed pod formation stage as the period with the most significant varietal differences, suggesting it may represent a critical regulatory window for cyanogenic glycoside synthesis. GO and KEGG enrichment analyses indicated that DEGs were primarily involved in metabolic processes (including secondary metabolism and carbohydrate metabolism), oxidoreductase activity, and transmembrane transport functions. Of these, the cytochrome P450 pathway was most significantly enriched at the full bloom stage (H2 vs. L2). A total of 15 LuCYP450 and 13 LuUGT85 family genes were identified, and their expression patterns were closely associated with cyanogenic glycoside accumulation: In high-cyanogenic varieties, LuCYP450-8 was continuously upregulated, and LuUGT85-12 was significantly activated during later stages. Conversely, in low-cyanogenic varieties, high expression of LuCYP450-2/14 may inhibit synthesis. These findings systematically reveal the genetic basis and temporal dynamics of cyanogenic glycoside biosynthesis in flax and highlight the seed pod formation stage as a decisive regulatory window for cyanogenic glycoside synthesis. This study provides new insights into the coordinated regulation of cyanogenic pathways and establishes a molecular foundation for breeding flax varieties with low CNG content without compromising agronomic traits.