Objectives: To investigate whether glioma isocitrate dehydrogenase (IDH) 1 mutation and vascular endothelial growth factor (VEGF) expression can be estimated by histogram analysis of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI).Methods: Chinese Glioma Genome Atlas (CGGA) database was wined for differential expression of VEGF in gliomas with different IDH genotypes. The VEGF expression and IDH1 genotypes of 56 glioma samples in our hospital were assessed by immunohistochemistry. Preoperative DCE-MRI data of glioma samples were reviewed. Regions of interest (ROIs) covering tumor parenchyma were delineated. Histogram parameters of volume transfer constant (K^trans) and volume of extravascular extracellular space per unit volume of tissue (V_e) derived from DCE-MRI were obtained. Histogram parameters of K^trans, V_e and VEGF expression of IDH1 mutant type (IDH1^mut) gliomas were compared with the IDH1 wildtype (IDH1^wt) gliomas. Receiver operating characteristic (ROC) curve analysis was performed to differentiate IDH1^mut from IDH1^wt gliomas. The correlation coefficients were determined between histogram parameters of K^trans, V_e and VEGF expression in gliomas.Results: In CGGA database, VEGF expression in IDH^mut gliomas was lower as compared to wildtype counterpart. The immunohistochemistry of glioma samples in our hospital also confirmed the results. Comparisons demonstrated statistically significant differences in histogram parameters of K^transand V_e [mean, standard deviation (SD), 50th, 75th, 90th. and 95th percentile] between IDH1^mutand IDH1^wtgliomas (P < 0.05, respectively). ROC curve analysis revealed that 50th percentile of K^trans (0.019 min⁻¹) and V_e (0.039) provided the perfect combination of sensitivity and specificity in differentiating gliomas with IDH1^mutfrom IDH1^wt. Irrespective of IDH1 mutation, histogram parameters of K^transand V_e were correlated with VEGF expression in gliomas (P < 0.05, respectively).Conclusions: VEGF expression is significantly lower in IDH1^mut gliomas as compared to the wildtype counterpart, and it is non-invasively predictable with histogram analysis of DCE-MRI.