Material testing, particularly in biological applications, can be an extensive endeavor leading to a significant investment of resources. This article details a simple material and coating testing assay series that provides insights into leaching, antibacterial, antifouling, and foul-release characteristics. The results of these methods can guide future research, applications, and development efforts by providing data from which to make informed decisions. A material or coating can be quickly assessed in industrial and academic settings with minimal resources by employing a set of benign, single-species direct-contact toxicity assays and simple spectroscopic and microscopic analysis methods. Herein, we demonstrate how this series of biological assays may be utilized and the potential interpretations of the results by using two-hybrid organo-silicon-based coatings.