At present, the role of agricultural practices on the dynamic of GHGs is being investigated worldwide. In this study, the effects of soil tillage practices (conventional vs. conservation techniques) and fertilization sources (inorganic vs. organic) on soil CO₂ emissions in durum wheat (Triticum durum Desf.)–potato (Solanum tuberosum L.) rotation in the Mediterranean area were evaluated. This study aimed to understand the changes in the soil carbon content and the soil CO₂ emissions under different soil tillage practices (moldboard plow (P), subsoiler (R), and spading machine (S)) and fertilization sources (mineral (M) and organic (O) with municipal organic waste). Soil CO₂ flux, soil water content, and soil temperature data were collected for both crops using a portable closed-chamber infrared gas dynamics system. Significant relationships were detected between soil CO₂ emissions and soil temperate and soil water content. However, these relationships were found only for durum wheat crops. Our findings indicate that including sustainable agricultural practices in wheat–potato rotation system could act as an appropriate alternative option to increase soil organic carbon, mitigate CO₂ emissions, and reduce the dependence on chemical inputs and energy.

Several strategies have been proposed recently to reduce the accumulated harmful circumstances of intensive conventional farming practices. The adoption of conservation soil tillage and organic fertilization techniques could contribute to achieving this goal. The study was conducted over a three-year trial to assess the effects of soil tillage practices (plough; subsoil; spading) and fertilization management (inorganic vs. organic) on durum wheat growth dynamics and productivity in a Mediterranean environment. The chlorophyll concentration (CC) was evaluated using an MC-100 chlorophyll meter, and Green Area (GA) was extracted from red-green-blue (RGB) digital images acquired with a commercially available digital camera. These non-destructive low-cost methods were used to examine crop status and its reactions to climate conditions over three consecutive growing seasons in the field. A positive strong linear relationship between both CC and GA against grain yield in each growing season was identified. The study highlights that CC and GA methods are valuable tools to assess crop development under Mediterranean conditions. The results indicated that the applied conservation tillage systems, such as subsoil tillage and using the spading machine, along with organic fertilization with compost, are valuable choices in the Mediterranean basin for sustainable and higher crop production (13%) compared to the conventional traditional system.

Within the European Union (EU), new plant varieties to be included in the Common catalog of a member state have to be registered on the national list after plant variety testing processes to establish whether the candidate variety is distinguishable, uniform, and stable (DUS) and meets the cultivation or use value requirement (VCU). Technical development, climate change, and changing consumer needs, including the detection of GMOs, necessitate the innovation of plant variety testing methods. In our study, we assessed new characters, testing methods, and inclusion of additional data for the potential to benefit the DUS and VCU protocols. To achieve our goal, we asked experts to fill in questionnaires for the DUS and VCU methods currently used for a selection of common crops, including potato, maize, lentil, oilseed rape, and perennial grass. Within the EU-funded “InnoVar” project, partners sent out questionnaires to 19 European Countries and to 3 countries outside Europe. Surveys were aimed at analyzing the strengths, weaknesses, opportunities, and threats (SWOT) of the current methods. With their help, it is possible to look for a new direction, opportunity, and strategy to incorporate, together with the innovative new techniques, into the development of the new methods. Our study demonstrated that the SWOT analysis could be used to achieve the set goals. Results obtained after evaluation of surveys confirmed that introduction of new characters such as cold tolerance, nitrogen and water efficiency, etc. has become necessary, as has the inclusion of new test methods (molecular markers, precision techniques, organic farming). The development of high-yielding, disease and/or pest-resistant plant varieties with good adaptability and the accurate evaluation of genotypes play a crucial role in ensuring that farmers can access high-performing plant varieties and contribute to sustainable food production.

Within the European Union (EU), new plant varieties, to be included at the Common catalog of a member state, has to be registered at the national list, after plant variety testing processes to establish whether the candidate variety is distinguishable, uniform and stable (DUS) and meets the requirement of the cultivation or use value requirement (VCU). Technical development, climate change and changing consumer needs, including the detection of GMOs, necessitate the innovation of plant variety testing methods. Within the EU-funded "InnoVar" project, it has been sent out questionnaires to several countries, which were aimed at analyzing strengths, weaknesses, opportunities and threats (SWOT) of the current methods and with their help it is possible to have look for a new direction, opportunity and strategy, which can incorporate together with the innovation results into the development of the new methods. Our study confirmed that the SWOT analysis could be used to achieve the set goals; according to the answers to the questionnaires, it became necessary to introduce new characters (e.g. cold tolerance) and new test methods (molecular markers, precision techniques, organic farming) into the variety testing procedures. The development of high-yielding, disease and/or pest-resistant plant varieties with good adaptability and the accurate evaluation of genotypes play a crucial role in ensuring that farmers can access high-performing plant varieties and contribute to sustainable food production.

Nowadays, arsenic (As) accumulation in agricultural soils and its transfer in crop yields is representing a growing concern that threatens food safety and security in the Mediterranean environment. Soil tillage and fertilization may increase the accumulation of As in plant tissues; therefore, there is a need to develop sustainable agronomical practices capable of supporting crop yield while mitigating As accumulation. The current study was carried out through a 7-year experiment with the aim of evaluating the As uptake by different parts of the durum wheat plant. The experimental treatments include the following: (i) three soil tillage practices (plowing, subsoiling, and spading) and (ii) two fertilization methods (mineral and organic). A factorial randomized complete block design with three replications was adopted. The experimental period refers to the 2018/2019, 2019/2020, and 2020/2021 growing seasons. The results suggest that the maximum level of As was found in plant roots and the minimum in wheat kernels. The chemical fertilization as 2020 × Mineral (1.522 mg As kg⁻¹ d.m.) and 2020 × Plowing (1.855 mg As kg⁻¹ d.m.) had the maximum As content in the roots. Conversely, the content of As was at a minimum in the wheat kernels for organic fertilization as 2021 × Organic (0.012 mg As kg⁻¹ d.m.) and subsoiling tillage as 2021 × Subsoiling (0.008 mg As kg⁻¹ d.m.). Moreover, the application of an organic fertilization source as a tool for enhancing the soil organic matter content also significantly decreased the As content. The results suggest that reduced tillage practices and the adoption of organic amendment could be classified as sustainable agronomic practices in agri-food systems, which are able to improve plant quality and assure a safe consumption of wheat kernels.

Nowadays, Arsenic accumulation in agricultural soils and its transfer in crop yields is representing a growing concern that threat to food safety and security in Mediterranean environment. Soil tillage and fertilization may increase the accumulation of Arsenic in plant tissues, therefore, there is the need to develop sustainable agronomical practices able to support crop yield while mitigate As accumulation. The current study carried out in a 7-year long-term experiment with the aim to evaluate As uptake by different plant parts of durum wheat. The experimental treatments are: (i) three soil tillage practices (plowing, subsoiling and spading) and (ii) two fertilization methods (mineral and organic). A factorial randomized complete block design with three replications was adopted. The experimental period refers to the 2018/2019, 2019/2020 and 2020/2021 growing seasons. The results suggested that the maximum level of Arsenic was found in plant roots and minimum was found in wheat kernels. The chemical fertilization as 2020×Mineral (1.522 mg As kg-1 d.m) and 2020×Plowing (1.855 mg As kg-1 d.m) had the maximum Arsenic content in roots. Conversely, the level of Arsenic content was minimum in wheat kernels for organic fertilization as 2021×Organic (0.012 mg As kg-1 d.m) and subsoiling tillage as 2021×Subsoiling (0.008 mg As kg-1 d.m.). Moreover, the application of organic fertilization source, as a tool for enhancing the soil organic matter content also significantly decreased the Arsenic level. The results suggests that reduced tillage practices and adoption of organic amendment could be identified as sustainable agronomic practices in agri-food systems able to improve the quality and assure a safe consumption of wheat kernels. Further studies will be required to evaluate the effectiveness of these agronomical practices also in other crops.