Accurately estimating aboveground biomass (AGB) is crucial for assessing carbon storage in forest ecosystems. However, traditional field survey methods are time-consuming, and vegetation indices based on optical remote sensing are prone to saturation effects, potentially underestimating AGB in subtropical forests. To overcome these limitations, we propose an improved approach that combines three-dimensional (3D) forest structure data collected using unmanned aerial vehicle light detection and ranging (UAV LiDAR) technology with ground measurements to apply a binary allometric growth equation for estimating and mapping the spatial distribution of AGB in subtropical forests of China. Additionally, we analyze the influence of terrain factors such as elevation and slope on the distribution of forest biomass. Our results demonstrate a high accuracy in estimating tree height and diameter at breast height (DBH) using LiDAR data, with an R² of 0.89 for tree height and 0.92 for DBH. In the study area, AGB ranges from 0.22 to 755.19 t/ha, with an average of 121.28 t/ha. High AGB values are mainly distributed in the western and central-southern parts of the study area, while low AGB values are concentrated in the northern and northeastern regions. Furthermore, we observe that AGB in the study area exhibits an increasing trend with altitude, reaching its peak at approximately 1650 m, followed by a gradual decline with further increase in altitude. Forest AGB gradually increases with slope, reaching its peak near 30°. However, AGB decreases within the 30–80° range as the slope increases. This study confirms the effectiveness of using UAV LiDAR for estimating and mapping the spatial distribution of AGB in complex terrains. This method can be widely applied in productivity, carbon sequestration, and biodiversity studies of subtropical forests.

The composition and spatial distribution of tree species are pivotal for biodiversity conservation, ecosystem productivity, and carbon sequestration. However, the accurate classification of tree species in subtropical forests remains a formidable challenge due to their complex canopy structures and dense vegetation. This study addresses these challenges within the Jiangxi Lushan National Nature Reserve by leveraging high-resolution GF-2 remote sensing imagery and UAV multispectral images collected in 2018 and 2022. We extracted spectral, texture, vegetation indices, geometric, and topographic features to devise 12 classification schemes. Utilizing an object-oriented approach, we employed three machine learning algorithms—Random Forest (RF), k-Nearest Neighbor (KNN), and Classification and Regression Tree (CART)—to identify 12 forest types in these regions. Our findings indicate that all three algorithms were effective in identifying forest type in subtropical forests, and the optimal overall accuracy (OA) was more than 72%; RF outperformed KNN and CART; S12 based on feature selection was the optimal feature combination scheme; and the combination of RF and Scheme S12 (S12) yielded the highest classification accuracy, with OA and Kappa coefficients for 2018-RF-S12 of 90.33% and 0.82 and OA and Kappa coefficients for 2022-RF-S12 of 89.59% and 0.81. This study underscores the utility of combining multiple feature types and feature selection for enhanced forest type recognition, noting that topographic features significantly improved accuracy, whereas geometric features detracted from it. Altitude emerged as the most influential characteristic, alongside significant variables such as the Normalized Difference Greenness Index (NDVI) and the mean value of reflectance in the blue band of the GF-2 image (Mean_B). Species such as Masson pine, shrub, and moso bamboo were accurately classified, with the optimal F1-Scores surpassing 89.50%. Notably, a shift from single-species to mixed-species stands was observed over the study period, enhancing ecological diversity and stability. These results highlight the effectiveness of GF-2 imagery for refined, large-scale forest-type identification and dynamic diversity monitoring in complex subtropical forests.