Introduction: Ovarian cancer is the third most common gynaecological cancer and has a very high mortality rate. The cornerstone of treatment is complete debulking surgery plus chemotherapy. Even with treatment, 80% of patients have a recurrence. Circulating tumour DNA (ctDNA) has been shown to be useful in the control and follow-up of some tumours. It could be an option to define complete cytoreduction and for the early diagnosis of recurrence. Objective: We aimed to demonstrate the usefulness of ctDNA and cell-free DNA (cfDNA) as a marker of complete cytoreduction and during follow-up in patients with advanced ovarian cancer. Material and Methods: We selected 22 women diagnosed with advanced high-grade serous ovarian cancer, of which only 4 had complete records. We detected cfDNA by polymerase chain reaction (PCR), presented as ng/mL, and detected ctDNA with droplet digital PCR (ddPCR). We calculated Pearson correlation coefficients to evaluate correlations among cfDNA, ctDNA, and cancer antigen 125 (CA125), a biomarker. Results: The results obtained in the evaluation of cfDNA and ctDNA and their correlation with tumour markers and the radiology of patients with complete follow-up show disease progression during the disease, stable disease, or signs of recurrence. cfDNA and ctDNA correlated significantly with CA125. Following cfDNA and ctDNA over time indicated a recurrence several months earlier than computed tomography and CA125 changes. Conclusion: An analysis of cfDNA and ctDNA offers a non-invasive clinical tool for monitoring the primary tumour to establish a complete cytoreduction and to diagnose recurrence early.

Aquatic organisms are exposed to multiple stressors, including microplastic pollution and rising temperatures. Bioplastics like Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are considered sustainable alternatives to conventional plastics, although their biological effects remain poorly understood. This study evaluated the effects of PHBV microplastics on Artemia franciscana under different temperature and exposure conditions. Organisms were exposed to 25 and 100 mg·L⁻¹ PHBV for 7, 14, and 21 days at 25 °C and for 14 days at 29 °C. Growth, development, antioxidant enzyme (CAT, GST) and esterase activities (ChE, CbE), lipid peroxidation (LPO), gut histology, fatty acid profiles and polymer particle length distributions were assessed. Growth and development increased with PHBV concentration, exposure time, and temperature. Enzymatic activities and LPO were significantly affected by these factors, although no evidence of oxidative damage was detected. Marked gut lesions were observed at 100 mg·L⁻¹ PHBV at 29 °C after 14 days. Fatty acid profiles were mainly influenced by time and temperature, while high PHBV levels were associated with additional, more subtle changes in long-chain polyunsaturated fatty acids. PHBV particle length distributions also varied depending on exposure conditions. These findings suggest that PHBV induces physiological responses distinct from those typically reported for conventional microplastics and highlight the importance of considering multiple stressors in ecotoxicological studies.