Chemokines and their receptors have been reported to drive immune cells into tumours or to be directly involved in the promotion or inhibition of the development of tumours. However, their expression in regional lymph node (LN) tissues in melanoma patients remains unknown. The present study investigated the relationship between the expression of mRNA of chemokines and their receptors and clinicopathology of the regional LN tissues of skin cutaneous melanoma (SKCM) patients available in The Cancer Genome Atlas. The relationship between chemokines and their receptors and the composition of immune cells within the tumour was analysed. In SKCM regional LN tissues, the high expression of 32 types of chemokines and receptors, namely CCL2, 4‐5, 7‐8, 13, 22‐25, CCR1‐9, CXCL9‐13, 16, CXCR3, 5, 6, XCL1‐2 and XCR1 in LN was associated with favourable patient prognosis. Conversely, high expression of CXCL17 was an indicator of poor prognosis. The expression of mRNA for CXCL9‐11, 13, CXCR3, 6, CCL2, 4, 5, 7, 8, 25, CCR1, 2, 5, and XCL1, 2 in regional LN tissues was positively correlated with the fraction of CD8‐positive T cells and M1 macrophages, and was negatively correlated with M0 macrophages. CCR4, 6‐9, CCL13, 22, 23 and XCR1 were positively correlated with the fraction of memory B cells and naive T cells, and negatively correlated with M0 macrophages and resting mast cells, suggesting that chemokines and their receptors may affect the prognosis of patients by guiding immune cells into the tumour microenvironment to eliminate tumour cells.

Background: Because the structure of eyebrows is complicated, students need a lot of practice to become familiar with the distribution pattern of eyebrow hair. Objectives: The current study was designed to investigate training methods for eyebrow design and planting practice before eyebrow hair transplantation to improve planting quality. Methods: Twelve surgical students were selected. The 6 students in the experimental group had never performed eyebrow transplantation surgery, whereas the 6 in the control group had experience of the procedure. Students in both groups received theoretical and simulated eyebrow hair transplantation instruction. Students were required to master standard eyebrow transplantation. The results of the experimental group and the control group were compared before and after training. Results: The results revealed that the overall results of the experimental and control groups significantly improved compared with before training (P < .05). After theoretical training, 2-dimensional simulation training, and 3-dimensional simulation training, eyebrow shape, symmetry, hair angle, and density after the 3-dimensional simulation training were statistically significantly improved (P < .05). There was no statistically significant difference, and the students in the control group did not demonstrate any improvement in their capacity to arrange the eyebrows or shape them symmetrically. After training, however, the angle and density of the hair substantially improved. Conclusions: The experimental group's technical ability in simulated eyebrow planting significantly improved. The present study demonstrated that the students could develop their skills and get closer to the level of surgeons with experience in eyebrow transplant surgery by integrating theoretical lectures with practiced procedures.