The antimicrobial activity of Allium ursinum aqueous extract prepared using high pressure extraction was evaluated. Minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) of A. ursinum extract for six bacterial pathogens were determined using the broth macrodilution method. Although the A. ursinum extract was shown to be effective toward all investigated foodborne bacteria, its antimicrobial activity depended on its concentration and bacterial strain. Listeria monocytogenes was the most sensitive to antimicrobial activity of A. ursinum extract among all tested pathogens. Accordingly, the lowest MIC and MBC of A. ursinum extract were determined for L. monocytogenes (28 and 29 mg/mL). The tested extract showed a similar antimicrobial potential to other examined bacterial strains (Salmonella Enteritidis, Proteus hauseri, Enterococcus faecalis and two strains of Escherichia coli) with MIC and MBC values at concentrations of 29 and 30 mg/mL, respectively. The dependence of the antimicrobial activity of the A. ursinum extract on the level of contamination of tested pathogens was also observed. The increase in the contamination level caused an intense reduction in antibacterial potential of the A. ursinum extract. The composition of the A. ursinum extract was analyzed and found to be a good source of polyphenols and sulfur compounds. However, considering the applied extraction method and the HPLC analysis of bioactive compounds, the antimicrobial potential may be attributed more to polyphenol content. The obtained results that the extracts have shown toward food pathogens open the possibility of using the tested extracts as natural additives in a variety of food products.

This study analyzed the phenolic compounds, organic acids, sugars, and antioxidant activity in different conventional apple cultivars (Malus domestica) from the Serbian market. Polyphenol profiles, sugars, and organic acid contents were analyzed by HPLC, and antioxidant activity was examined by DPPH and FRAP. Notable findings included variations in phenolic compound presence, with certain compounds detected only in specific cultivars. ‘Red Jonaprince’ exhibited the highest arbutin (0.86 mg/kg FW) and quercetin-3-rhamnoside content (22.90 mg/kg FW), while ‘Idared’ stood out for its gallic acid content (0.22 mg/kg FW) and ‘Granny Smith’ for its catechin levels (21.19 mg/kg FW). Additionally, malic acid dominated among organic acids, with ‘Granny Smith’ showing the highest content (6958.48 mg/kg FW). Fructose was the predominant sugar across all cultivars. Chemometric analysis revealed distinct groupings based on phenolic and organic acid profiles, with ‘Granny Smith’ and ‘Golden Delicious’ exhibiting unique characteristics. Artificial neural network modeling effectively predicted antioxidant activity based on the input parameters. Global sensitivity analysis highlighted the significant influence of certain phenolic compounds and organic acids on antioxidant activity.

Plant growth regulators (PGRs), such as cytokinins (6-benzyladenine; BA) and gibberellins (GAs), are widely used in fruit production. This study focused on the plum cultivar “Čačanska rodna” (P. domestica L.) grafted on vegetative rootstock “WaVit” and generative rootstock Prunus cerasifera, with interstock Prunus spinosa. PGR treatments included 50 mg L⁻¹ and 100 mg L⁻¹ of BA and 200 mg L⁻¹ of gibberellin A3 (GA₃) and non-treated control. Pomological characteristics of plum fruits were examined at harvest, while physicochemical properties were analyzed at harvest and after 28 days of cold storage and 4 days of shelf life. GA₃ and BA application changed the fruit morphological traits, improved skin strength, and increased carotenoid, anthocyanin and sucrose content while decreasing the titratable acidity at harvest. The beneficial effects of higher sucrose, anthocyanin and carotenoid levels persisted in all PGR-treated fruits after cold storage and shelf life. GA₃-treated fruits had firmer flesh, stronger skin and higher total soluble solids (TSS) content, while in BA-treated plums, these effects were rootstock-dependent. The physical properties and chemical composition of plum fruit in the postharvest period suggest beneficial effects of the applied PGR treatments. Moreover, these chemical treatments might have prolonged the beneficial impact on fruit storability, nutritional profile and sensory properties. Based on our results, GA₃ preharvest treatment can be included in standard cultivation practices within contemporary production systems of European plums not only to improve fruit quality at harvest but also to improve the storage potential and nutritional value, regardless of the rootstock used.

Interaction of grafting and shading on tomato physical properties and chemical composition after 15 days of storage at 10 °C and 90% relative humidity was investigated in ungrafted and grafted tomato cultivars ‘Optima F₁’ and ‘Big beef F₁’ grown under shading nets (red and pearl net) and nonshaded conditions. For grafted plants ‘Maxifort’ rootstock was used. The effects of two weeks of storage was statistically significant when taking into account the effects of grafting, shading and variety for all tomato fruit composition parameters, except total phenols. A principal component analysis demonstrated that the changes in tomato fruit traits during the studied storage period were the main source of differentiation in tomato fruit quality. Beside a slight loss of firmness, tomato fruits were generally expected to have lower lycopene, sugar, malic and citric acid contents, higher succinic acid content, more elastic fruit skin and higher ascorbic acid content. Additionally, after storage, fruits from grafted plants had lower total phenol, higher ascorbic acid and higher succinic acid contents compared to fruits from ungrafted plants. Storage diminishes the differences in quality achieved through convenient grafting and shading combinations.