The genus Trichoderma is widely recognized for its ability to secrete lignocellulosic enzymes, establishing it as a crucial technological resource for the bioconversion of agro-industrial waste biomass via fermentation. This review explores the potential of utilizing lignocellulosic waste from diverse industries as a sustainable nutrient source for producing Trichoderma spp. and various desirable metabolites via fermentation. Significant emphasis is placed on the enzymatic activities of Trichoderma species in two critical stages of second-generation biofuel production. Firstly, in the pre-treatment stage to break down complex polysaccharides of lignocellulosic biomass, thereby enhancing production efficiency and yield, and, secondly, during the hydrolysis process to produce fermentable sugars essential for biofuel production. Additionally, this review discusses other applications of Trichoderma fermentation, such as enhancing animal feedstock nutrition and employing its spores as biocontrol agents. Ongoing research efforts are directed at optimizing fermentation protocols, identifying suitable waste substrates, and genetic manipulation of strains to enhance the economic viability of Trichoderma’s biotechnological applications. This manuscript contributes to the field of circular biotechnology by offering a detailed review of recent progress on the integration of agro-industrial waste materials in Trichoderma-based bioconversion technologies, highlighting both current achievements and future research directions necessary to enhance the economic and environmental sustainability of waste biomass utilization.

The global demand for local and high-quality food sources has increased in recent decades, driven by consumer preferences and a growing population. RAS, hydroponics and a ‘hybrid’ version, aquaponics, are food production techniques that could support such growing demand. The current study evaluated coupled aquaponics (Jade perch and lettuce) with and without mineral supplementation in comparison to standard cultivation methods, i.e., RAS and hydroponics. The mineral supplementation in aquaponics was carried out using a hydroponics commercial blend fertilizer (10% of the dosage utilised in the hydroponics treatment). Fish/plant performance, nutrient dynamics and water quality were evaluated for 9 weeks using twelve experimental units (~720 L in total; fish tank/reservoir + filtration systems/sump + floating raft-based plant tank). After two crops of plants, the results showcased the positive impact of mineral supplementation in aquaponics; however, the hydroponics treatment outperformed the two aquaponics treatments, e.g., with higher total plant wet weight and total plant length (p < 0.05). Fish in aquaponics with and without mineral supplementation presented similar growth performance as compared to RAS (p > 0.05). By comparing these different farming approaches, this study sought to provide insights into optimising aquaponics systems and advancing the integration of Jade perch into aquaculture practices.

Amoebic gill disease (AGD), caused by Neoparamoeba perurans, is a major health challenge for Atlantic salmon aquaculture globally. While freshwater bathing for 2 hr is effective in reducing infection severity, there is need for more rapid and lower cost alternatives. To this end, a combination of sodium percarbonate (SPC) in freshwater was examined for its treatment efficacy. Initial in vitro studies showed a reduction in amoeba viability when exposed for 30 min to freshwater containing >500 mg/L SPC. Subsequently, AGD‐affected salmon were bathed for 30 min in 16°C freshwater containing 100, 500 or 1,000 mg/L SPC, or for 2 hr in 16°C freshwater to mimic industry practice. Treatment at the highest SPC concentration caused extensive gill damage and substantial mortality. Neither occurred to a significant extent at lower SPC concentrations. Gill pathology of surviving fish 10 days post‐treatment (dpt) was comparable to or more severe than pre‐treatment, and significantly (p < .001) more severe than in 2 hr freshwater bathed fish. N. perurans DNA was confirmed by qPCR in all treatment groups at 10 dpt. The data indicate that a 30‐min exposure to SPC in freshwater is not a suitable alternative to existing freshwater treatment of AGD.