Cryopreservation plays a critical role in animal breeding and the conservation of endangered species, but it often compromises sperm characteristics such as morphology, motility, and viability due to oxidative stress. This study explores the antioxidative effect of Mito-Tempo (MT) and Berberine (BER) to enhance post-thaw sperm quality in rabbits. Pooled rabbit sperm samples were supplemented with different concentrations (0.0, 0.5, 5, 10, 50 µmol/L) of MT and BER. Sperm motility was evaluated using computer-assisted semen analysis, while viability, apoptosis, reactive oxygen species (ROS) levels, acrosome integrity, and mitochondrial function were assessed through flow cytometry. The results revealed that MT at 5 and 10 µmol/L and BER at 10 µmol/L significantly improved total and progressive motility, mitochondrial activity, and sperm viability compared to the control group. Furthermore, 10 µmol/L BER enhanced acrosome integrity, while both 5 µmol/L MT and 10 µmol/L BER effectively reduced ROS levels and apoptosis. This study is the first to demonstrate the protective effects of MT and BER on rabbit sperm during cryopreservation. By mitigating oxidative stress and reducing apoptosis, these antioxidants markedly improved post-thaw sperm quality, positioning MT and BER as promising agents for improving sperm cryosurvival.

The closing of schools due to COVID-19 was a critical incident that should have caused a rethinking of education in our country. Among the many changes that this crisis has brought, one is fully remote teaching. Our research focuses on a comparison of the changes between on-site and remote forms of biology teaching, the opinions and feelings of teaching staff across all the institutional levels, and their opinions regarding the usage of online teaching tools in the future. The research shows that teachers have used both time-tested teaching aids and modern technology to generate an environment that would be as close to on-site teaching as possible. Similarly, the teachers with longer teaching experience had felt a greater degree of stress during the remote teaching period. Teachers of primary and tertiary schools agree that they can imagine having a combined form of education in the future but that the practical classes of biology must be completed on-site. On the other hand, most secondary school teachers want to preserve only the on-site form of teaching. Our study provides information on the current state of coping with the pandemic situation in Slovakia from teachers’ perspectives.

The policy of the European Union on land management promotes sustainable agriculture with an emphasis on the protection of biodiversity and the environment. Organic agriculture is the most appropriate alternative to ensure this common goal. The aim of this study was to determine the influence of factors such as pH, moisture, nitrogen potassium, phosphorus and grass herbaceous vegetation on the spatial structure of epigeic arthropods during the spring and summer seasons under organic farming conditions. Research took place between 2020 and 2022, and we recorded 14,988 individuals belonging to 16 taxa using pitfall traps. Between the years 2020 and 2022, we confirmed a decrease in the number of individuals and taxa of epigeic arthropods from the grass herbaceous vegetation to the interior of the field during the summer seasons. This decline was not confirmed in the spring seasons. Phosphorus, potassium, nitrogen, moisture and pH factors also had a significant influence on the spatial structure of epigeic arthropods. Our results show that the higher number of individuals and taxa at the grass herbaceous vegetation occurred only during the summer period. This fact contributes to an increase in biomass and, consequently, the yield of crops.

The floodplain forests of the Danube Delta are among the important European biotopes and are protected in Slovakia under Natura 2000. In order to preserve these biotopes, their restoration is underway, which also restores the original fauna. These biotopes are sensitive to environmental and ecological changes, which is also reflected in the spatial distribution of epigeic arthropods. Between the years 2020 and 2023, we investigated the impact of floodplain restoration on the population structure of epigeic arthropods in eight study areas (two control study areas and six study areas with ongoing biotope restoration). We placed five pitfall traps in a transect for each biotope. In total, we recorded 66,771 individuals belonging to 15 arthropod taxa. We found differences in the taxonomic structures between forest stands with management and forest stands without management (larger number of taxa) using spatial modelling. We also confirmed interannual changes in the taxa composition of epigeic arthropods and their abundance. Over the years of restoration, the number of individual epigeic arthropods decreased. In the years following revitalization, when succession took place, it subsequently increased. Overall, the restoration management of floodplain forests had a positive effect on epigeic arthropods, as well as on their number of individuals, which is important for the preservation of these important habitats in Europe.

The aim of this research was to record changes in the population structure of epigeic spider assemblages in the Central European Danube Delta (Slovakia) as a result of habitat management measures and the impact of human intervention. During this research (2020–2023), we assessed the impact of management measures on newly planted forest stands and the effect of grazing in semi-natural conditions, and carried out diversity monitoring in flooded meadows. A total of 6344 individuals belonging to 89 spider species were collected by pitfall traps and identified. Using spatial modelling, we observed the following: (i) there are differences between the structures of managed and unmanaged forest stands (larger number of taxa); (ii) the differences in the number of individuals between study plots and years were statistically significant; (iii) the trend analysis of spider communities showed that study plots that underwent management intervention are expected to see an increase in the number of individuals in the future; and (iv) in the areas that did not undergo management, the number of species was stable. Using spiders as bioindicators could therefore answer the question of whether anthropogenic disturbance disrupts ecological stability. This approach utilizes spiders to assess the sustainability of the landscape.

Soil fauna represent an important component of terrestrial ecosystems and serve as sensitive bioindicators of environmental changes and the intensity of anthropogenic disturbances. Our research was conducted between 2020 and 2023 across 15 habitats differing in management intensity. Soil fauna were sampled using pitfall traps. A total of 88,112 individuals belonging to 18 taxa were recorded. Community variability was assessed using redundancy analysis (RDA), which revealed a clear differentiation between managed and unmanaged habitats. The results indicated that more soil fauna preferred more stable and less disturbed environments, whereas managed sites exhibited greater heterogeneity of soil fauna communities. Statistical analyses confirmed significant differences in individual abundance among study areas (1–15), years (2020–2023), and seasons (Friedman test, p = 0.016; Kruskal–Wallis test, p < 0.001), highlighting the substantial influence of habitat characteristics and seasonal dynamics on the structure of soil fauna. The predictive models suggested the persistence of natural population fluctuations without a pronounced long-term decline in abundance. The findings provide valuable information for optimizing habitat management, evaluating the effectiveness of restoration measures, and supporting biodiversity conservation in ecosystems of European importance.