Lavandula dentata, popularly known as lavender, is commonly used in traditional medicine for the treatment of digestive and inflammatory disorders. The objective of this study was to analyzed the chemical oil composition, antioxidant and antimicrobial activities of the essential oil and anatomical markers of the leaf and stem of L. dentata cultivated in South Brazil. Essential oil showed an antioxidant activity similar to rutin and gallic acid when analyzed by phosphomolybdenum method. However, by the free radical DPPH and ABTS methods, it showed a slight potential antioxidant. Essential oil presented 1,8-cineol (63%) as major component, antimicrobial activity against Gram-positive, Gram-negative bacteria strains and Candida albicans, by broth microdilution. The anatomical profile provided the following main microscopic markers: hypostomatic leaves; diacytic stomata, thin and striate cuticle; multicellular and branched non-glandular trichomes; capitate glandular trichomes; peltate glandular trichomes; dorsiventral mesophyll; flat-convex shape midrib, truncated on the abaxial side; one collateral vascular bundle in the midrib; square stem shape, angular collenchyma alternated with cortical parenchyma; sclerenchymatic fibers well-developed on the four edges.

Keywords: green synthesis; metallic nanoparticles; myelogenous leukemia line; cytotoxic potential; Lamiaceae

Introduction: Third generation of LED light curing units might be used in short exposure periods for orthodontic brackets bonding. Objective: This study evaluated the effect of the different radiant exposure (RE) values: Manufacturers’ instructions (MI), ½ MI, 1/4 MI and Turbo mode. Two third-generation LED curing units were used: VALO® and Bluephase 20i® . The degree of conversion (DC) and Vickers hardness (VHN) of an orthodontic composite (OC) (Transbond XT) under metallic (MB) or ceramic brackets (CB) were measured. Methods: OC was applied to the bracket base, which was then placed over an attenuated total reflectance (ATR) table coupled to an infrared light spectroscope, or to a glass surface for the VHN analysis. The specimens were light-cured and DC values were calculated. The VHN was obtained in a microhardness tester. The data were analyzed with 2-way ANOVA followed by Tukey’s post-hoc test (pre-set α=0.05). Linear regression analysis evaluated the relationship between RE values and dependent variables. Results: CB allowed higher DC and VHN values than MB (p< 0.001). No significant difference was noted among groups when CB were used. For MB, MI groups showed the highest DC and VHN values. A significant, but weak relationship was found between delivered RE values and dependent variables. Conclusions: The decrease in RE values from third generation LED CU did not jeopardize the DC values when CB were used, but can compromise DC and VHN values when MB are used.

Curcuminoids (CURs) are frequently co-administered with piperine (PIP) to enhance their pharmacological potential. Curcumin (CUR), the principal phenolic compound in the rhizome of Curcuma longa L. (turmeric), has limited oral bioavailability, while PIP can act as a bioenhancer. In this context, reliable simultaneous quantification of CURs-PIP is essential for routine quality control in herbal medicine industry. This study aimed to develop and validate a simple, precise, and robust RP-HPLC/DAD method for the simultaneous determination of CURs and PIP in spray-dried microparticles. Microparticles were produced by spray-drying using Eudragit® S100, and the analytical method was validated according to ICH Q2(R1) and ANVISA RDC 166/2017. Specificity, linearity, precision, accuracy, robustness, and encapsulation efficiency were assessed. Linearity was demonstrated within 200-500 μg·mL⁻1 (CURs expressed as curcumin, CUReq) and 8-20 μg·mL⁻1 (PIP) with r>0.996. Recoveries were ~100% (100.18% for CUReq; 99.56% for PIP) with CV<5%. Encapsulation efficiency was ≥99% for both analytes. The method proved accurate, precise, robust, and suitable for routine quality control of microparticulate formulations containing both CURs and PIP.