Grain weight is one of the most important components of cereal yield and quality. A clearer understanding of the physiological and molecular determinants of this complex trait would provide an insight into the potential benefits for plant breeding. In the present study, the dynamics of dry matter accumulation, water uptake, and grain size in parallel with the expression of expansins during grain growth in wheat were analysed. The stabilized water content of grains showed a strong association with final grain weight (r²=0.88, P <0.01). Grain length was found to be the trait that best correlated with final grain weight (r²=0.98, P <0.01) and volume (r²=0.94, P <0.01). The main events that defined final grain weight occurred during the first third of grain-filling when maternal tissues (the pericarp of grains) undergo considerable expansion. Eight expansin coding sequences were isolated from pericarp RNA and the temporal profiles of accumulation of these transcripts were monitored. Sequences showing high homology with TaExpA6 were notably abundant during early grain expansion and declined as maturity was reached. RNA in situ hybridization studies revealed that the transcript for TaExpA6 was principally found in the pericarp during early growth in grain development and, subsequently, in both the endosperm and pericarp. The signal in these images is likely to be the sum of the transcript levels of all three sequences with high similarity to the TaExpA6 gene. The early part of the expression profile of this putative expansin gene correlates well with the critical periods of early grain expansion, suggesting it as a possible factor in the final determination of grain size.

The yield of 24 commercial varieties and accessions of common bean (Phaseolus vulgaris) has been determined at different sites in Chile and Bolivia. Statistical analysis was performed in order to characterize whether a particular variety was more or less stable in yield under different environmental conditions. Amongst these, two varieties have been identified for more detailed study: one variety has a higher than average yield under unstressed conditions but is strongly affected by stress, and another has a reduced yield under unstressed conditions but is less affected by stress. The contrasting rate of abscission of the reproductive organs under drought stress was clearly consistent with these differences. The more tolerant genotype shows a great deal of plasticity at the biochemical and cellular level when exposed to drought stress, in terms of stomatal conductance, photosynthetic rate, abscisic acid synthesis, and resistance to photoinhibition. By contrast, the former lacks such plasticity, but shows an enhanced tendency for a morphological response, the movement of leaves, which appears to be its principal response to drought stress.

SUMMARY: Agricultural systems are challenged by global climatic change in a scenario of increasing food demand by a growing population. The increase in average temperature will be the main environmental factor affecting the crop development and productivity worldwide, although changes in carbon dioxide (CO₂) concentration and rainfall are also expected. Global warming in the range of moderately high temperatures (15–32°C) is projected for temperate environments such as that of central-southern Chile, where grain crops such as wheat are widely grown. The present study assessed the impact of moderately high temperatures on both yield and quality traits of wheat during key stages for grain number and grain weight determination. Two cultivars of spring wheat (Pandora INIA and Huayún INIA) were grown under field conditions during two cropping seasons (2006/07 and 2007/08) under different thermal regimes, consisting of a combination of three temperatures (a control at ambient temperature and two increased temperature treatments, ranging from 2·6 to 11·7°C above the control) and two (3–15 and 20–32 days after anthesis) or three (booting to anthesis (Bo-At), 3–15 and 20–32 days after anthesis) timing regimes. The data recorded showed that the extent of yield reduction was strongly dependent on the timing of the heat treatments. Increased temperature at pre- (Bo-At) or early post-anthesis (3–15 days after anthesis) affected grain yield the most (reducing it by 8–30%). In light of these results, yield reductions of up to 18% can be expected when the crop undergoes average temperature increase of 2·8°C at Bo-At. In this study, the negative effect of increasing temperature on grain yield was associated with both grain number and grain weight reductions; however, different sensitivities to higher temperatures were found between cultivars. Although protein concentration of grains was not affected by higher temperatures, other negative effects on industrial quality traits are important, considering the impact of thermal treatments on grain size of both cultivars.

The effects of salt on the quality of fruits were investigated in order to compare the impact of salt on key fruit properties of the cultivated domesticated tomato species (Solanum lycopersicum) and its wild halophyte relative Solanum chilense. To this end, cherry tomato plants (S. lycopersicum var. cerasiforme) and from accession LA4107 (S. chilense) were maintained for 112 days in the absence or presence of NaCl (40 and 80 mM) in nutrient solution. Among others, salinity decreased fruit weight and increased total soluble solid (TSS) in S. lycopersicum but not in S. chilense. The fruit antioxidant capacity estimated by ferric reducing antioxidant power (FRAP) analysis was higher in S. chilense than in S. lycopersicum and increased in the former while it decreased in the latter in response to NaCl. Salinity increased the lycopene (LYC) content but decreased ß-carotene (b-CAR) concentration in the fruits of S. lycopersicum, while these compounds were not detected in the wild halophyte S. chilense. The oxidative status of salt-treated fruits was more tightly regulated in S. chilense than in S. lycopersicum. The two considered species, however, possess complementary properties and interspecific crosses may therefore be considered as a promising option for the improvement of salt-stress resistance in tomatoes.