Rumen development in calves is affected by many factors, including dietary composition. MicroRNAs (miRNAs) are known to function in the development of the rumen in cattle, what is not known is how these miRNAs function in rumen development of calves fed with high and low ratios of non-fibrous carbohydrate (NFC)/neutral detergent fiber (NDF). A total of six healthy Charolais hybrids bull calves of similar weight were divided into two groups; three calves were fed a mixed diet with NFC/NDF = 1.35 (H group), and three were fed a mixed diet with NFC/NDF = 0.80 (L group). After 105 days on the diet, calves were sacrificed and rumen tissues were collected. Tissues were subjected to histological observation and miRNA expression analysis. Functional enrichment analysis was conducted on the target genes of the miRNAs. Targeting and regulatory relationships were verified by luciferase reporter assay and quantitative PCR (qPCR). We found that the length of rumen papilla in the L group was significantly greater than that in the H group, while the width of rumen papilla in H group was significantly greater than that that in L group. We identified 896 miRNAs; 540 known miRNAs, and 356 novel predicted miRNAs. After statistical testing, we identified 24 differentially expressed miRNAs (DEmiRNAs). miRNA-mRNA-cluster network analysis and literature reviews revealed that cell proliferation, differentiation, physical and nutrient stimuli processes participate in rumen development under different NFC/NDF levels. The regulatory relationships between three DEmiRNAs and five target genes were verified by examining the levels of expression. The binding sites on bta-miR-128 for the peroxisome proliferator activated receptor gamma (PPARG) and solute carrier family 16 member 1 (SLC16A1) genes were investigated using a dual luciferase assay. The results of this study provide insight into the role of miRNAs in rumen development in calves under different NFC/NDF levels.

In order to investigate the effects of fasting caecotrophy on hepatic lipid metabolism in rabbits, 12 weaned female New Zealand white rabbits were randomly divided into (n = 6/group) a control and fasting caecotrophy group. Rabbits in the experimental group were treated with an Elizabeth circle to prevent them from eating their own soft feces for a 60-day period. Growth and blood biochemical indices, transcriptome sequencing and histology analysis of the liver were performed. Compared with the control group, final weight, weight gain, liver weight, growth rate and feed conversion ratio, all decreased in the experimental group (p < 0.05). RNA sequencing (RNA-seq) analysis revealed a total of 301.2 million raw reads (approximately 45.06 Gb of high-quality clean data) that were mapped to the rabbit genome. After a five-step filtering process, 14,964 genes were identified, including 444 differentially expressed genes (p < 0.05, foldchange ≥ 1). A number of differently expressed genes linked to lipid metabolism were further analyzed including CYP7A1, SREBP, ABCA1, GPAM, CYP3A1, RBP4 and RDH5. The KEGG (Kyoto Encyclopedia of Genes and Genomes) annotation of the differentially expressed genes indicated that main pathways affected were pentose and glucuronide interactions, starch and sucrose metabolism, retinol metabolism and PPAR signaling. Overall, the present study revealed that preventing caecotrophy reduced growth and altered lipid metabolism, both of which will help guide the development of new approaches for rabbits’ feeding and production. These data also provide a reference for studying the effects of soft feces in other small herbivores.

Probiotics play an important role in animal production, providing health benefits to the host by improving intestinal microbial balance. In this study, we added three different probiotics, Saccharomyces cerevisiae (SC), Bacillus licheniformis (BL), and lactic acid bacteria (LAB), and compared them with the control group (CON), to investigate the effects of probiotic supplementation on growth performance, gut microbiology, and gut flora of S. trutta. Our results showed that feeding probiotics improved the survival, growth, development, and fattening of S. trutta. Additionally, probiotic treatment causes changes in the gut probiotic community, and the gut flora microorganisms that cause significant changes vary among the probiotic treatments. However, in all three groups, the abundance of Pseudomonas, Acinetobacter, and Rhizophagus bacterial genera was similar to that in the top three comparative controls. Furthermore, differences in the composition of intestinal microbiota among feed types were directly associated with significant changes in the metabolomic landscape, including lipids and lipid-like molecules, organic acids and derivatives, and organoheterocyclic compounds. The probiotic treatment altered the gut microbiome, gut metabolome, and growth performance of S. trutta. Using a multi-omics approach, we discovered that the addition of probiotics altered the composition of gut microbiota, potentially leading to modifications in gut function and host phenotype. Overall, our results highlight the importance of probiotics as a key factor in animal health and productivity, enabling us to better evaluate the functional potential of probiotics.

To investigate the spatiotemporal distribution characteristics of nitrogen and phosphorus in the water and sediment of the Danjiangkou Reservoir, the source of the Middle Route of China’s South-to-North Water Diversion Project, we designed a year-long monitoring program. The water and sediment samples were collected from 13 sampling points in the upstream and downstream areas over the year. The results revealed significant spatial heterogeneity in N and P concentrations, with higher levels of total nitrogen, nitrate nitrogen, and nitrite nitrogen in the upstream area compared to the downstream area (p < 0.01). Total phosphorus was also significantly higher in the upstream area (p < 0.05). Seasonal variations were observed, with TN and TP levels peaking in February and August, respectively. The TN:TP ratio indicated a severe P-limited state in most periods, transitioning to a co-limited state of N and P during summer. Sediment analysis showed that TN and TP concentrations were higher in the upstream area, with no significant differences between upstream and downstream on an annual basis, exhibiting strong stoichiometric internal stability. However, seasonal differences were noted, particularly in February and November. This study highlights the complex interactions between water and sediment, emphasizing the role of sediment resuspension, water flow, and seasonal changes in nutrient dynamics. These findings provide a scientific basis for the management and protection of water quality in the Danjiangkou Reservoir, ensuring its role as a critical water source for the South-to-North Water Diversion Project.

Rice–crayfish co-culture is a dominant aquaculture mode in China, while the regional heterogeneity in crayfish nutritional quality remains insufficiently clarified. This study collected rice-field-cultured crayfish samples from six major producing provinces (Henan, Hubei, Hunan, Jiangxi, Jiangsu, and Anhui) to systematically evaluate their meat yield, conventional nutritional components, amino acid and fatty acid profiles, and mineral element contents. Significant regional differences were observed in all measured indicators. Jiangsu crayfish showed the highest meat yield (22.97% in females, 20.72% in males), crude protein (18.5%), and total amino acids (16.59 g/100 g). Jiangxi samples had the highest crude fat (0.7%), ash (1.5%), and calcium (1280 mg/kg). Anhui crayfish exhibited the highest polyunsaturated fatty acid (PUFA) content (47.30%). Hubei recorded the highest total eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) (22.41%). Henan crayfish were highest in EPA (19.6%) and manganese (3.72 mg/kg), while Hunan samples had the highest iron (9.06 mg/kg). Valine was identified as the first limiting amino acid in all provinces, with secondary limiting amino acids varying regionally. Despite regional differences in specific nutritional indices, crayfish from all six provinces consistently exhibited high protein, low fat, balanced amino acid profiles, abundant unsaturated fatty acids, and rich essential minerals, with all measured nutritional mineral contents within national food safety limits. The findings provided empirical data and a scientific basis for crayfish quality evaluation, origin characteristic analysis, and targeted nutritional optimization of farming formulas, as well as for the differentiated development of regional crayfish industries.

Aeromonas salmonicida is a major pathogenic bacterium that poses a significant threat to salmonid fish. Yadong County, located in the Xizang Autonomous Region, is renowned for its characteristic industry of Salmo trutta aquaculture. In recent years, the outbreak of Bacterial Gill Disease (BGD) has led to substantial economic losses for S. trutta farmers. Our prior research identified A. salmonicida as one of the primary culprits behind BGD. To mitigate the impact of A. salmonicida on S. trutta, we conducted a comprehensive study aimed at identifying genes associated with resistance to A. salmonicida. This involved transcriptome sequencing and 16S rRNA sequencing of intestinal flora, providing valuable insights for the study of disease resistance in S. trutta. In this study, we identified 324 genera with 5171 ASVs in the susceptible group and 293 genera with 5669 ASVs in the resistant group. Notably, Methylobacterium and Sphingomonas were common bacteria present in the salmon’s gut, and their proportions remained relatively stable before and after infection. Shewanella, with its antagonistic relationship with Aeromonas, may play a crucial role in the salmon’s defense against A. salmonicida. Several related genes were identified, including angptl4, cipcb, grasp, ccr9a, sulf1, mtmr11, B3GNT3, mt2, PLXDC1, and ank1b.