Defensins constitute a family of cationic antimicrobial peptides that act against different bacteria; however, global information regarding their antibacterial mechanisms from omics-based analyses is highly limited. In this study, transcriptomics and proteomics were used to explore the antibacterial mechanisms of defensin (BmKDfsin4) originally isolated from a scorpion on a common Gram-positive bacterium. Staphylococcus aureus (AB94004) was treated with BmKDfsin4 for 15, 30, or 45 min based on its ability to moderately inhibit bacterial growth for one hour. Compared with those in the control group, more than 1000 genes and nearly 500 proteins in S. aureus were significantly differentially expressed after BmKDfsin4 treatment. In-depth analysis revealed that BmKDfsin4 significantly upregulated bacterial ribosome-related pathways and ribosomal components. In contrast, BmKDfsin4 also significantly downregulated the synthesis and metabolism pathways of bacterial amino acids. Moreover, BmKDfsin4 inhibited the synthesis pathways of teichoic acid and peptidoglycan, which are the key components of the cell wall in S. aureus. Furthermore, glycolysis and other metabolic processes in S. aureus were markedly reduced by BmKDfsin4. Overall, the global information detected from S. aureus revealed the multiple antibacterial mechanisms of BmKDfsin4, which could encourage the exploration of global bacterial information from the defensin family with high degrees of sequence variability and accelerate the research and development of defensins as new antibacterial agents.

Ecdysis is a well-known developmental feature among arthropods. Because the aggregate and synchronous molting of first-instar scorpions is markedly different from the common independent molting behavior of older scorpions and most arthropods, knowledge on the biological benefits of the unusual behavior of first-instar scorpions remain limited. Before the molting of newborn scorpions, their mothers exhibited a remarkable ability to efficiently locate the fallen offspring and help them climb onto their back, which was supported by strong maternal behavior because they climbed more swiftly than the 7-day postpartum scorpions. Most newborn scorpions molted and survived on the mother’s back, with a survival rate of approximately 100%, and most newborn scorpions survived via aggregate molting behavior on sand in the absence of mothers (89.83% ± 1.91%). The important role of the mother scorpion was further highlighted in mothers with one to five first-instar scorpions. While all first-instar scorpions individually or reciprocally molted and survived on the mother’s back, only 52.00% ± 7.14% to 79.20% ± 4.24% of newborn scorpions isolated from the mother could individually or reciprocally molt and survive on the sand, and the aggregated states of first-instar scorpions strengthened as their numbers on sand increased before molting. These results highlight collaborative molting as an evolutionary driving force for newborn scorpions. Taken together, both maternal care and collaborative aggregate molting behavior enhanced the survival of first-instar scorpions before and after molting, and these benefits for first-instar scorpions play essential and evolutionary roles in scorpion survival.