Oncidium hybridum is one of the important cut-flowers in the world. However, the lack of aroma in its cut-flower varieties greatly limits the sustainable development of the Oncidium hybridum cut-flowers industry. This paper is an integral investigation of the diel pattern and influencing factors of the aroma release of Oncidium Twinkle ‘Red Fantasy’. GC-MS analysis revealed that the release of 3-Carene peaked at 10:00, while Butyl tiglate and Prenyl senecioate did so at 14:00, with a diel rhythm. By analyzing the correlation network between aroma component synthesis and differentially expressed genes, 15 key structural genes were detected and regulated by multiple circadian rhythm-related transcription factors. Cluster-17371.18_TPS, Cluster-65495.1_TPS, Cluster-46699.0_TPS, Cluster-60935.10_DXS, Cluster-47205.4_IDI, and Cluster-65313.7_LOX were key genes in the terpenoid and fatty acid derivative biosynthetic pathway, which were co-expressed with aroma release. Constant light/dark treatments revealed that the diurnal release of 3-Carene may be influenced by light and the circadian clock, and Butyl tiglate and Prenyl senecioate may be mainly determined by endogenous circadian clock. Under constant light treatment, the TPS, DXS, IDI, and LOX genes seem to lose their regulatory role in the release of aroma compounds from Oncidium Twinkle ‘Red Fantasy’. Under constant dark treatment, the TPS genes were consistent with the release pattern of 3-Carene, which may be a key factor in regulating the diel rhythm of 3-Carene biosynthesis. These results laid a theoretical foundation for the study of floral transcriptional regulation and genetic engineering technology breeding of Oncidium hybridum.

Urban secondary wilderness (USWs) is a near-natural place in cities and is an important link to reconnect humans with the natural world. Assessing the perceptual elements of USWs not only provides insight into public perceptions and preferences, but also helps to further explore its relevance to experiential values such as environmental restoration benefits. In this study, we selected three USWs cases located in Fuzhou, the capital city of Fujian Province, China, and constructed an evaluation system containing two types of dimensions and seventeen perceptual elements. Based on the public’s evaluation of the importance of and satisfaction with the perceived elements, we extracted the key perceived elements and analyzed their relationship with environmental restoration benefits. The results indicated that all three USWs cases scored over 4.9 on the Perceived Restoration Scale (PRS). Plants, Landscape color, Water, Landform, Climate, Freedom, and Naturalness are the key perceptual elements of the USWs, and the correlation coefficients between the satisfaction with the above seven perceptual elements and the scores of the PRS were all positive. In summary, USWs is an ideal place for residents to carry out restoration activities, and its key perceptual elements play a driving role.