Eucalyptus is an important plantation tree with a high economic value in China. The tree contributes significantly to China’s timber production. The stable and efficient Eucalyptus regeneration system and genetic transformation system are of great significance for exploring the regulatory function and possible genetic breeding capacity of important genes in the species. However, as a woody plant, Eucalyptus has problems, such as a long generation cycle, strong specificity of the regeneration system, and a low genetic conversion rate, which seriously limit the rapid development of Eucalyptus genetics and breeding programs. The present review summarizes the status of research on Eucalyptus regeneration and genetic transformation, with a focus on the effects of explants, media, plant growth regulators (PGRs), and concentrations in the Eucalyptus regeneration process. In addition, the effects of genotype, Agrobacterium, antibiotics, preculture, and co-culture on the genetic transformation efficiency of Eucalyptus are discussed. Furthermore, the study also summarizes the problems encountered in Eucalyptus regeneration and genetic transformation, with reference to previous studies, and it outlines future developments and prospects. The aim was to provide a reference for solving the problems of genetic instability and the low transformation efficiency of eucalyptus, and to establish an efficient and stable eucalyptus regeneration and transformation system to accelerate the process of its genetic improvement.

GATA transcription factors play an important role in plant growth and devel- opment, nutrient metabolism and environmental stress responses. Currently, there are few studies on the gene families of mangrove plants. In this study, bioinformatics methods were used to identify GATA family genes in Acanthus ilicifolius, and their subfamily classification, gene structure and phylogenetic characteristics were analyzed. The structure showed that a total of 95 GATA family genes were identified and divided into 4 subfamilies, and they had GATA conserved functional domains. Analysis of physical and chemical properties showed that its ORF was 231-1653 bp, the number of encoded amino acids was 77-551 aa, and the theoretical isoelectric point was 4.9-10.8. Gene structure and motif analysis showed that GATA genes in the same subfamily maintained consistent gene structure and conserved motifs, and amino acid homology alignment showed that there was a GATA conserved zinc finger domain. Chromosomal localization GATA transcription factors are distributed on 42 chromosomes of Acanthus ilicifolius, and a pair of biosynthetic cluster genes were found in the chromosome 34. Transcript data and qRT-PCR results showed that GATA expression was tissue-specific, GATA expression was strongly induced under high salinity stress, and there was expression was significantly up-regulated in roots at 12 h and significantly expressed in stems at 48 h. These results provide a theoretical and scientific basis for exploring the regulatory functions of GATA gene in the growth and development of Acanthus ilicifolius.