Design and synthesis of a novel series of 4a,5,6,11-tetrahydro-4H-thieno[3′,2′:5,6]pyrido[2,3-a]carbazol-4-one derivatives (3a–3g), and evaluation of their anticancer activity (HeLa, MCF-7 and HEp-2 cell lines by MTT assay method) and antioxidant (DPPH and ˙OH radical scavenging) activity. The designed compounds (3a–3g) were synthesized using microwave irradiation in the presence of p-TsOH, with yields up to 91%, in a short time (11 min). Halogenated compounds (3e and 3f) strongly bind with the calf thymus (CT)-DNA helix via intercalation and show more hypochromicity (84% and 81% respectively) with a red shift (12 nm and 10 nm, respectively). Compounds (3e and 3f) completely cleaved pBR322 DNA by oxidative cleavage. In general, it was observed that chloro and bromo substituted compounds 3e and 3f show excellent anticancer activity and strongly bind to CT-DNA, which may be due to the stronger electron withdrawing tendency of the chloro and bromo groups than that of the electron donating and unsubstituted carbazole derivatives. The most potent compounds 3e (2.05, 3.35, and 10.24 fold) and 3f (3.62, 6.28 and 8.09 fold) displayed stronger cytotoxicity than the positive control cisplatin, and also compared with ellipticine. Compound 3g with –OCH₃ substitution and 3e with chloro substitution exhibited greater antioxidant activity than standard ascorbic acid. All the designed compounds were well accommodated in the ATP-binding site of human CK2 protein (Asn118, Ile174, Val53, Val116). The adopted design strategy provided two potent halogenated compounds (3e and 3f), which may require further in vivo investigations before subjecting to clinical trials, and also some useful insights about the future design of more potent inhibitors for the treatment of cancer.

A new charge transfer (CT) complex benzimidazolium-4-methylbenzenesulfonate formed between benzimidazole and 4-methylbenzenesulfonic acid has been studied spectrophotometrically in methanol at room temperature. The binding of the novel CT complex with calf thymus DNA has been investigated by adsorption spectrum, the establish the ability of the CT complex for its interaction with the chosen DNA. The elemental stiochiometry and the X-ray crystallographic structure of the complex were studied, showing the significance of sulfonate anion in holding stability. Powder XRD confirms the crystalline nature of the compound. NMR signals explicitly confirmed the molecular structure crystal. Antioxidant activity of the CT complex was found to be potent. TG-DTA studies also proves the stability of the synthesized complex.