Chemical pesticides used against insect pests of stored food have adverse effects on both health and the environment. So, the present study aims to evaluate the insect repulsive and insecticidal power of Ruta chalepensis L. essential oil (EO) from the region of Oulmes (Central plateau of Morocco); the ultimate objective is to develop a biological and ecological control strategy against pests. Thus, the EO obtained by hydrodistillation from the aerial parts of Ruta chalepensis L. was identified by GC‐MS; its repellent and fumigant toxicity effects on adults of Tribolium castaneum Herbst were, respectively, investigated by the preferential area method on a filter paper and the inhalation test. The insecticide power was estimated by determining the percentage of mortality as a function of the duration of exposure and concentration of the EO. The essential oil obtained is characterized by the dominance of 2‐undecanone (64.35%), piperonyl piperazine (11.9%), 2‐decanaone (5.12%), 2‐dodecanone (4.52%), decipidone (3.9%,) and 2‐tridecanone (2.36%). This EO is endowed with a very repulsive power belonging to class V, which is strongly due to its majority compound 2‐undecanone. The dose 0.038 μl/ml gave a repellent power of 100% after 15 min. The tests also revealed a considerable insecticidal effect, which reached 100% after 48 hours at a dose of 0.62 μl/ml. The calculation of the lethal dose causing 50% mortality (LD50) and the lethal times after which there is 50% mortality (LT50) allowed deducing that the insecticidal effect of Ruta chalepensis L. is time‐ and dose‐dependent. Hence, the effectiveness of Ruta chalepensis L. EO attests that it can constitute a healthy alternative to fight against Tribolium castaneum Herbst.
Morocco is a significant botanical reservoir that boasts a wealth of raw materials with promising applications across various industrial sectors, notably in pharmaceuticals and food. The objective of this study was to assess the effectiveness of essential oils (EOs) derived from Laurus nobilis L. leaves originating from the Tanger (EOT) and Meknes (EOM) regions in combating Callosobruchus maculatus infection. The chemical compositions of these oils were examined using Fourier transform infrared (FTIR) spectroscopy and gas chromatography–mass spectrometry (GC-MS). The biological activity of the EOs was evaluated via repulsion and fumigation tests against C. maculatus at varying concentrations. FTIR analysis revealed distinct vibrational bands indicative of various chemical compounds. GC-MS analysis was used to delineate the major chemical constituents of the EOs. The three predominant compounds in the EOT were 1,8-cineole (37.64%), linalool (16.40%), and adamantane (12.00%), whereas 1,8-cineole (47.84%), toluene (17.60%), and α-phellandrene (8.44%) were the most abundant in the EOM. Notably, the EOs exhibited significant repellent activity against C. maculatus, with repulsion percentages ranging from 51.11 to 90.00% in Tanger and 67.78 to 93.33% in Meknes. Mortality rates varied from 0 to 100% depending on the treatment. However, the mean concentrations showed mortality rates ranging from 29.44 to 65.56% for the EOT and from 21.11 to 67.78% for the EOM, with LD50 values of 11.96 μL/L and 5.22 μL/L. Docking studies revealed that 1,8-cineole had the highest binding affinity for the active site of acetylcholinesterase, thus confirming its toxic activity against C. maculatus. The findings of this study highlight the ability of EOs extracted from L. nobilis in the Moroccan regions of Tanger and Meknes to act as effective insecticides and repellents against C. maculatus, thereby highlighting avenues for further exploration of pest management and agricultural practices.
27 July 2023 in International Journal of Pest Management
Reducing reliance on synthetic herbicides is essential to protect health, preserve ecosystems and limit resistant weeds. A two-season field experiment in Meknes, Morocco, assessed the effects of plane tree leaves, oat straw and black plastic mulches on faba bean growth, weed control and diversity. Weed infestation was highest in unweeded plots. All mulches reduced weed density and biomass, with control rates of 80.37%, 69.51% and 84.50%, respectively. Yields rose by 43.46% with plane leaves, 41.53% with oat straw and 50.84% with black plastic. Mulching lowered species number and the Margalef index at flowering. The Shannon–Wiener index remained unchanged at maturity, indicating no loss of overall diversity. Although black plastic was most effective, organic mulches gave similar results. Given the risk of soil microplastic contamination, plant-based options offer a safer, more sustainable alternative. Mulching is a practical and eco-friendly method for weed control and yield improvement in faba bean cultivation.